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WorksheetsRevision UPS3
Total questions: 62
Worksheet time: 46mins
Gene mutation usually occurs during:
DNA repair
DNA replication
Cell division
DNA transcription
A mutation in which only one nucleotide is altered is called a:
Frameshift Mutation
Deletion Mutation
Point Mutation
Insertion Mutation
When do random, or spontaneous, mutations happen?
During cell division
When cells are exposed to hazardous chemicals
When cells are exposed to certain viruses
When cells are exposed to radiation
What type of mutation is shown in the diagram?
Point mutation - missense
Frameshift Mutation - deletion
Frameshift Mutation - addition
Point mutation - nonsense
A type of mutation that affect every codon beyond the point of mutation.
Point Mutation
Silent Mutation
Nonsense Mutation
Frameshift Mutation
What type of mutation is this?
Nonsense mutation
Missense mutation
Silent mutation
Frameshift mutation
The original gene is ABCDEF. The new sequence of gene is AEDCBF. What is the type of chromosomal mutation occurred?
Insertion
Inversion
Deletion
Translocation
ATTTGAGCC- Original
ATTGAGCC - Mutated
The example above is an example of a
Insertion
Point mutation
Deletion
Substitution
Identify which of the following is a genetic mutation disease..
Common head cold
Cystic fibrosis
Heart attack
Chicken pox
Which of the following is an example of a beneficial mutation?
When an organism's lifespan becomes shorter.
When an organism becomes unable to reproduce.
When an organism's body becomes deformed.
When an organism develops immunity to a disease.
What mutation has occurred here?
T-G-A-C-C-A
T-G-A-G-C-A
Substitution
Deletion
Insertion
Frameshift
DNA molecule segment is : TTACGCAAG
The mutated DNA segment is TTACGCAAC. This is an example of ___ mutation.
Substitution
Insertion
Inversion
Translocation
ATT-TGA-GCC- Original
ATT-GAG-CC - Mutated
The example above is an example of a
Insertion- Frameshift
Deletion- Substitution
Deletion -Frameshift
All of the above
What type of gene mutation has occurred here?
- Normal -AGA-TTC-ATA-GCG
- Mutant-AGA-TTC-AAT-AGC-G
deletion frameshift
insertion frameshift
substitution
nonsense
Why are insertion and deletion mutations so harmful?
They change all of the codons from the mutation on down the line, which changes the amino acid sequence
They insert things that an organism doesn't need.
They often delete things that organisms need.
Insertion and deletions are not any more harmful than substitution mutations.
Which of these are the effect if base substitution mutation? Tick all correct answers
Missense
Silence
Nonsense
Frameshift
If a nucleotide is substituted yet the amino acid produce is the same, the effect of mutation is ___________
silence
missense
nonsense
frameshift
If a nucleotide is substituted, and it change one amino acid to to another. This effect is called
Missense
Nonsense
Silence
Frameshist
In sickle-cell anemia..
Glutamic acid is substituted with valine on Beta-polypeptide chain
Valine is substituted with glutamic acid on Beta-polypeptide chain
Glutamic acid is substituted with valine on alpha-polypeptide chain
Valine is substituted with glutamic acid on alpha-polypeptide chain
The polypeptide produced is truncated and short. The most likely type of point mutation that causes this is
Base substitution - nonsense mutation
Base substitution - missense mutation
Insertion mutation
Inversion mutation
The insertion of a base pair into the genetic code will cause frameshift mutation, unless the number of the base pairs inserted is
one
two
three
four
A nonsense mutation
Usually results in the formation of an abnormally short polypeptide
causes one amino acid to be changed to another
caused by insertion mutation
resulted from deletion of one or two bases
This individual suffers from which chromosomal genetic disorder?
Klienfelter's Syndrome
Down Syndrome
Cris-du-Chat Syndrome
Andrew's Syndrome
Nondisjunction
When a segment of a chromosome breaks off.
When a segment of a chromosome breaks off, flips, and reattaches.
When a segment of a chromosome is copied or doubles.
When a segment of a chromosome breaks and attaches to a non homologous chromosome.
When entire chromosomes don't separate evenly during meiosis.
Nondisjunction in Meiosis I is more deleterious than nondisjunction in Meiosis II
True
False
Why is nondisjunction in Meiosis I is more deleterious than nondisjunction in in Meiosis II?
All gametes formed are abnormal
75% of the gametes formed are abnormal
50% of the gametes formed are abnormal
25% of the gametes formed are normal
Polyploidy refers to
Multiple ribosomes present on a single mRNA
a chromosome which has replicated but not divided
an individual with extra sets of chromosomes
extra copies of gene adjacent to each other on a chromosome
Which one of the following is a polyploidy cell
1n
2n
4n
non of them
polyploidy is important to the field of
medicines
embryology
agriculture
physics
Human cell with 3n is
big cell
dead cell
cancer cell
skin cell
How to produce fertile individuals from sterile individuals with unusual number of chromosomes?
Double the chromosomes number
Reduce the chromosomes number
These are the reasons why sterile offspring may be produced from fusion of two different species.. EXCEPT
No homologous pair
No meiosis
No gamete
No mitosis
Which of the followings is an example of modifying enzymes?
BamH1
SMA1
DNA ligase
DNA polymerase
I ) It can replicate by itself
II ) Hybrid plasmid is introduced into bacteria by transformation
III ) Other than bacteria, plasmid can also be found in bacteriophage
Which restriction enzyme listed below was isolated from E. coli bacteria?
EcoRI
HindIII
HaeII
SmaI
Which of these is the sequence recognised by EcoR1?
5'- GAATTC -3'
3'- GAATTC- 5'
3'- CAATTG- 5'
5'- CAATTG-3'
Which of these sequences is recognised by SMA1
5'- CCCGGG -3'
5'- CCGCGG -3'
5'-GGGCCC -3'
5'-GGCGCC -3'
What type of cut is this photo?
Blunt
sticky
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells.
IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V) Use ligase to seal plasmid DNA to non-plasmid DNA
Following transformation and amplification, the bacteria with NO plasmid will...
Not forming colony
Will stain blue
Will stain white
Will form patches
Following transformation and amplification, the bacteria with ORIGINAL plasmid will...
Not forming colony
Will stain blue
Will stain white
Will form patches
Following transformation and amplification, the bacteria with RECOMBINANT plasmid will...
Not forming colony
Will stain blue
Will stain white
Will form patches
Which structure of plasmid that is important for selection of transformed bacteria with plasmid?
ampR gene
Ori
lacZ
GAATTC restriction site
Tick all correct answers.
Insertion of target genes...
Will cause the lacZ gene unable to be expressed
Will cause the lacZ gene able to be expressed
Will cause the bacteria to be stained blue
Will cause the bacteria to be stained white
Why is it critical to cut both DNA with target genes and plasmid by using the same restriction enzymes?
To produce nucleotide ends that are complementary to each other
To produce one recombinant plasmid with no nucleotide gaps
To allow screening to occur
To allow transformation to happen
Which enzyme completes the production of double-stranded cDNA?
DNA polymerase
Reverse transcriptase
DNA ligase
RNA polymerase
Which of these is used by reverse transcriptase as template to produce cDNA?
mRNA
template strands of DNA
codding strands of DNA
rRNA
Tick all correct answers on what makes cDNA is suitable to be used in genetic engineering
Represent the genes without exons
It can be used directly to express the desired protein
Derived from processed mRNA
Gene can be expressed without damaging the DNA
