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WorksheetsIB Transcription And Translation HL
Total questions: 40
Worksheet time: 24mins
The first three DNA bases of a gene are TAC.
What will be the anticodon of the tRNA molecule that corresponds to this?
TAC
UAC
ATG
AUG
Which of the following are true of a promoter?
I. it is a non-coding base sequence
II. RNA polymerase attaches to it
III. it is found on mRNA
I and II only
I and III only
II and III only
I, II and III
What is the name given to a non-coding DNA base sequence?
intron
exon
gene
repressor
When is pre-mRNA modified to form mature mRNA?
during transcription
just after transcription
during translation
just after translation
What happens to exons when pre-mRNA is modified?
they are removed
they are copied
they are spliced together
they are activated
What part of a ribosome first attaches to the mRNA in the cytoplasm?
A site
P site
large subunit
small subunit
What site on a ribosome does the first tRNA occupy?
A site
P site
E site
R site
What site on a ribosome does the second tRNA first occupy?
A site
P site
E site
R site
Which amino acid is coded for by the start codon on mRNA?
leucine
methionine
glycine
histidine
Between which sites on a ribosome does a peptide bond form between amino acids?
A and P
E and P
A and E
small subunit and large subunit
A ribosome moves one codon along the mRNA.
Which of the following is now not true?
the tRNA in the P site moves to the E site
the tRNA in the A site moves to the P site
the A site is initially empty
the E site is now empty
Which amino acid is coded for by the stop codon on mRNA?
methionine
isoleucine
phenylalanine
it does not code for an amino acid
Which site on the ribosome do release factors attach to?
A site
P site
E site
none of these
Which site on the ribosome is the protein released from?
A site
P site
E site
small subunit
Human genes can be transferred to bacteria.
The bacteria then produce and secrete human proteins.
Why is this possible?
Bacteria have ribosomes
Bacteria use amino acids
The genetic code is degenerate
The genetic code is universal
What is not true of mature mRNA in eukaryotes?
It has a poly-A tail at the 3' end
It is capped by a methyl group at the 5' end
It is made in the cytoplasm
It contains only exons
Humans can produce antibodies of many different shapes to deal with all possible pathogens that they may encounter.
What makes this possible?
Humans have a gene for each antibody molecule
Different combinations of introns are spliced together when forming mRNA
Different combinations of introns and exons are spliced together when forming mRNA
Different combinations of exons are spliced together when forming mRNA
Which of the following is/are true of introns?
One, some or all answers could be correct.
they are removed from pre-mRNA
they are non-coding base sequences
the promoter contains introns
the terminator contains introns
TRUE or FALSE:
A promoter is found on mRNA.
TRUE
FALSE
TRUE or FALSE:
The DNA sense strand has a complementary base sequence to mRNA.
TRUE
FALSE
In what direction is mRNA made?
5' --> 3'
3' --> 5'
5' --> 3' and 3' --> 5'
5' --> 5' and 3' --> 3'
"During transcription, free RNA bases attach to the DNA antisense strand by complementary base pairing.
The antisense strand acts as a template".
How many marks do you think this would be given in an exam?
0
1
2
3
TRUE or FALSE:
Prokaryotic DNA does not contain introns.
TRUE
FALSE
Arrange the following statements in the order that they occur during translation.
I. a tRNA anticodon attaches to a mRNA codon
II. tRNA-activating enzyme attaches a specific amino acid to tRNA
III. ribosome reaches a stop codon
I --> II --> III
IIII --> II --> I
II --> I --> III
II --> III --> I
Which of the following best describes a polysome?
a group of two or more ribosomes linked together
a group of two or more mRNA molecules linked together
a group of two or more ribosomes translating mRNA simultaneously
a group of two or more nucleosomes linked together
What is the role of tRNA activating enzyme?
To attach the anticodon to a tRNA molecule
To attach a tRNA molecule to the codon on mRNA
To remove the amino acid from a tRNA molecule
To attach an amino acid to a tRNA molecule
Which of the following is true of mRNA?
It is complementary to the antisense strand and made from 5' --> 3'
It has the same base sequence as the antisense strand and is made from 5' --> 3'
It is complementary to the antisense strand and made from 3' --> 5'
It has the same base sequence as the antisense strand and is made from 3' --> 5'
Which of the following is/are true of pre-mRNA?
I. it contains a 3' poly-A tail
II. it has a 5' methyl cap
III. it contains introns and exons
I and II only
II only
III only
I, II and III
Which of the following is/are true of mature mRNA?
I. it contains a 3' poly-A tail
II. it has a 5' methyl cap
III. it only contains exons
I and II only
II only
III only
I, II and III
In what order so the following events happen during translation?
I. tRNA attaches to mRNA
II. small subunit of ribosome attaches to mRNA
III. large subunit of ribosome attaches to mRNA
I --> II --> III
II --> III --> I
I ---> III --> II
II --> I --> III
RNA polymerase attaches to a (a) site at the start of a gene.
The (a) strand of DNA acts as a template in transcription.
RNA polymerase and the mRNA produced detach at the (a) site.
At the end of transcription, the two (a) DNA strands go back together. However, in DNA replication this does not happen.
A tRNA carrying a new amino acid usually attaches to the (a) site of a ribosome.
A tRNA carrying the growing polypeptide chain is usually in the (a) site of a ribosome.
A tRNA that has lost its amino acid leaves the (a) site of a ribosome.
How is transcription different to DNA replication?
One, some or all answers could be correct.
Transcription copies only part of a DNA strand
Transcription happens in the nucleus
Transcription produces a single-stranded product
Transcription uses only one template strand
Which of the following properties of mature mRNA help it to leave the nucleus?
exons
introns
poly-A tail
methyl group
Which of the following functions are carried out by RNA polymerase during transcription?
I. removal of introns by splicing
II. separation of DNA strands
III. joining RNA nucleotides to form mRNA
I and II only
I and III only
II and III only
I, II and III
