Wayground logo

Free Printable Worksheets

Font size

S
M
L
XL
Worksheets

practical molecular biology

Total questions: 26

Worksheet time: 22mins

Name
Class
Date
1.

Which dye is used for viewing the DNA under UV light?

a)

Ethidium bromide

b)

proflavin

c)

acridine orange

d)

methylene blue

2.

Once DNA has been extracted from the nucleus of a cell, it is exposed to ______ ______ that act like biochemical scissors to cut the DNA.

a)

restriction enzymes

b)

gel agarose

c)

clotting factors

d)

electric shock

3.
Gel electrophoresis enables scientists to
a)
separate DNA fragments.
b)
combine DNA fragments.
c)
count the genes in DNA.
d)
insert DNA in cells.
4.
During gel electrophoresis, DNA moves from the ___ end to the ___ end.
a)
positive, negative
b)
negative, positive
5.

How are buffers used in Electrophoresis?

a)

Conduct electricity

b)

Remove salts

c)

Add extension energy

d)

Dissolve excess DNA

6.

Made up of fragments of the same length

a)

Band

b)

Lane

c)

Well

d)

Tank

e)

Marker / DNA Ladder

7.

A chamber that the gel is place inside

a)

Band

b)

Lane

c)

Well

d)

Tank

e)

Marker / DNA Ladder

8.

In gel electrophoresis, the largest DNA fragment will appear

a)

closest to the starting wells

b)

farthest from the starting wells

c)

three quarters away from the starting wells

d)

it depends on how many fragments there are

9.
Which DNA fragment bands move faster and farther in a gel electrophoresis? 
a)
there is no way to tell 
b)
the largest ones
c)
the smallest ones
d)
none of these
10.

The uptake of naked DNA by bacteria is called :

a)

Conjugation

b)

Transformation

c)

Transfection

d)

Transduction

11.

Most commonly used matrix in gel electrophoresis is

(a) (b) (c) (d)

a)

ethidium bromide

b)

agarose

c)

polyacrylamide

d)

sulphate bromide.

12.

Which of the following is common in sticky ends and molecular probes?

a)

They both are parts of RNA molecules.

b)

They both involve complementary pairing.

c)

They both are important aspects of bacterial sex.

d)

They both are used as gene vectors in genetic engineering.

13.

For a DNA to function as a cloning vector the most essential requirement is -

a)

multiple restriction sites

b)

several selectable markers

c)

circular nature

d)

'ori' sequence

14.

Who discovered Restriction Enzymes?

a)

a).Watson and Crick

b)

b).Arber ,Natha, Linn

c)

c).Jacob and Monod

d)

d).Beadle and Tatum

15.

How do we name restriction Enzymes ?

a)

a).First letter in Cap for Genus, next two letter in small for species, next one letter for strain and roman numeral for order of discovery

b)

b). First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and English numeral for order of discovery

c)

c). First two letter in Cap for Genus, next one letter in capital for species, next one letter for strain and roman numeral for order of discovery

d)

d). First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and roman numeral for order of discovery

16.

The recognition sites for restriction enzymes are :

a)

a).Tautomer

b)

b). Pallindrome

c)

c).Metamere

d)

d).Acronym

17.

Restriction Enzymes were primarily used as :

a)

a).Defence by Protozoa against antibiotics

b)

b).Tool by Virus to cleave Bacterial genome

c)

c)Defence by Bacteria against bacteriophages viruses

d)

d).All of the above

18.

Restriction enzymes belong to class of enzymes:

a)

a).Peptidase

b)

b).Amylases

c)

c).Lipases

d)

d).Nucleases

19.

What is the term given to symmetrical ends of DNA after being cut by a restriction enzyme?

a)

Blunt Ends

b)

Sticky Ends

c)

Ligated Ends

d)

Restrictive Ends

20.
Some restriction enzymes produce "sticky ends". Sticky ends are . . . 
a)
single-stranded DNA "overhangs" that can bind to complementary DNA
b)
smooth cuts that are sticky due to the "goo" left behind by the enzyme
c)
The two wires (black and red) on a gel box
d)
The surface of the LB agar plates so that bacteria can grow
21.
In a molecule of double-stranded DNA, the amount of Adenine present is always equal to the amount of
a)
cytosine
b)
guanine
c)
thymine
d)
uracil
22.
What determines the code, or information, of a DNA molecule?
a)
the shape (structure) of the nitrogen bases
b)
the order (sequence) of the nitrogen bases
c)
the color of the nitrogen bases
d)
the frequency (number) of nitrogen bases
23.

Type of cut?

a)

sticky

b)

blunt

24.

What type of cut?

a)

sticky

b)

blunt

25.

What are the fragment legenths?

a)

8 , 24, 10

b)

8, 15, 19

c)

7, 37, 7

d)

7, 17, 14, 11

26.
Which is true about plasmids?
a)
Plasmids can be cut at specific sequences called restriction sites.
b)
Plasmids contain a promoter sequence that defines where transcription begins.
c)
Plasmids can contain an antibiotic resistance gene.
d)
All of the statements are true about plasmids.

Similar Resources on Wayground

DNA E RNA

23 questions

DNA E RNA

quiz

Worksheet

•

University

THYROID GLAND FUNCTION

21 questions

THYROID GLAND FUNCTION

quiz

Worksheet

•

University

ScienceQuiz

21 questions

ScienceQuiz

quiz

Worksheet

•

5th Grade - University

Silviculture

21 questions

Silviculture

quiz

Worksheet

•

University

Doctor quiz

22 questions

Doctor quiz

quiz

Worksheet

•

4th Grade - Professio...

Science Review (Photosynthesis)

22 questions

Science Review (Photosynthesis)

quiz

Worksheet

•

KG - Professional Dev...

Spine Exam Quiz

22 questions

Spine Exam Quiz

quiz

Worksheet

•

University

Translation of Amino Acids

22 questions

Translation of Amino Acids

quiz

Worksheet

•

10th Grade - University