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WorksheetsJournal Club
Total questions: 26
Worksheet time: 18mins
How many papers are the subject of today's JC?
0
1
2
3
Which one did you prefer?
Lomakin
Venturini
I don't know
What's the common topic of those two papers?
Endothelial cells
Mechanosensing
Migration
Nucleus
What does the cell do in response to excessive compression?
(a)
Do the two papers find a similar mechanism?
Yes
No
Don't know
When the nucleus is compressed, what happens to it?
Its membrane unfolds
It changes volume
It gets stiffer
Its membrane stretches
What does cPLA2 do?
Interact with C3PO
Requires Ca2+ to activate
Inserts itself between lipids in stretched membranes
Increases the production of arachidonic acid
What does the arachidonic acid do?
Scares people
Increases actin polymerization
Increases myosin 2 activity through ATP
Send people on acid trips
Myosin 2 activity is linked the contractility of the cortical actin network
Yes
No
Don't know
Can you repeat the question please?
The increased cortical tension leads to
Blebbing
Increased force on the AFM
Increased cytosolic pressure
Switch to amoeboid migration
The increased migration enables
Cells to escape confined spaces
Embryonic cells to place themselves
Cancer cells to migrate in dense tumor environment
Immune cells to be more efficient
Are the two papers using the same methods?
Yes
No
I don't know
Which one used human epithelial cells?
Lomakin
Venturini
Neither
Both
What was the compression threshold for triggering blebbing in Lomakin?
(a)
Which one used AFM as the cell compression method?
Lomakin
Venturini
Neither
Both
Does each paper have experiments that only they did?
Yes
No
I don't know
La réponse D
Who investigated if compression of the cell cytoplasm lead to the same effect?
Lomakin
Venturini
Neither, how can you not compress the highest organelle?
Both
Who investigated the role of the nuclear enveloppe state?
Lomakin
Venturini
Neither
Both
Who investigated the role of cell size vs nucleus size?
Lomakin
Venturini
Neither
Both
Who investigated the impact of nucleus size variability?
Lomakin
Venturini
Neither
Both
Who investigated compression of cells in a native matrix?
Lomakin
Venturini
Neither
Both
Who investigated another method of nuclear membrane stretching (through hypotonic swelling) ?
Lomakin
Venturini
Neither
Both
Who investigated the role of endoplasmic reticulum to plasma membrane distance?
Lomakin
Venturini
Neither
Both
Do you think it is a good idea to publish similar papers in the same journal edition?
Yes
No
No opinion
Debatable
Do the two papers differ in their "branding", i.e. what field their result impacts?
Yes
No
I don't know
Did you notice anything special in the "writing style" of these papers?
