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WorksheetsBiosafety and Synthetic Bio
Total questions: 12
Worksheet time: 24mins
Why was the BAX gene used in the lab?
Most readily available
Induction of apoptosis is the activity needed for treatment
Induction of non-apoptosis is the activity needed for treatment
It is most most easily manipulated in cancer patients
Why is a promoter needed?
To recruit RNA polymerase
To recruit DNA polymerase
To recruit Topoisomerase
To recruit Helicase
Which sequence is cut by the restriction enzyme BP clonase?
attB1
attP2
Both
Neither
How are donor vectors usually identified?
ppatient
pcancer
pdonr
pflk
What is Next generation sequencing used for?
To predict future outcomes of offspring
To determine functionality of a gene
To measure gene expression
To map out the human genome
What are the att sites?
Specific sequence that act like ligase
Specific sequences that are recognized and recombined by clonase
Specific sequences that removed from the new assembled sequence
Specific sequences that elongate and copy the DNA
At what temp was the clonase kept?
20 C
15 C
5 C
4 C
What techniques was used to introduce the new DNA into the bacteria
Gel electrophoresis
Denaturation
Electroporation
Exocytosis
How long did it take to incubate the single colony in the LB broth?
20 mins
1 hr
16 hrs
24 hrs
Why was it necessary to add a buffer to the spun down cells?
To dissolve the cell membrane for DNA extraction
To balance the sample back after lysis
For resuspension
All of the above
What is another name for sticky ends?
Tacky ends
Overhanging ends
Forced ends
Blunt ends
Blunt ends were created by which restriction enzymes for the lab?
EcoRI
BpIII
DpnI
HaeIII
