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BIOTECH- Protein structure & engg.

Total questions: 15

Worksheet time: 15mins

Name
Class
Date
1.

Active form of chymotrypsinogen contains___________ number of amino acids.

a)

244 amino acids interrupted into three peptides.

b)

244 amino acids interrupted into two peptides.

c)

245 amino acids interrupted into three peptides.

d)

246 amino acids interrupted into three peptides.

2.

The main substrate of chymotrypsin includes

a)

F,W,Y

b)

F,W,L

c)

F,W,M

d)

F,R,Y

3.

Few examples of organophosphate derivates are

a)

DTT and parathion

b)

Diazinon and phorate

c)

marathon and brufen

d)

Anacin and diazinon

4.

How many fragments would result if the following peptide is digested with enzyme trypsin.


LLYRTWYQBSDKLY

(a)  

5.

The solvent system used in electrophoretic technique in peptide mapping is

a)

Ethanol, water, acetic acid

b)

Butanol, water, ascorbic acid

c)

Butanol, water, acetic acid

d)

Butanol, ethanol, acetic acid,

6.

2D gel electrophoresis techniques was proposed by

a)

Linus pauling

b)

Max perutz

c)

V.M.Ingram

d)

O'Farrel

7.

A single substitution of a nucleotide (SNP) on coding strand of beta globin gene of hemoglobin results in sickle cell anemia. which of the following is TRUE with regard to the above .

 

a)

Codon GTG  transcribed to  GAG  translated to  GUG

b)

Codon GAG  transcribed to  GTG  translated to  GAG

c)

Codon GAG  transcribed to  GTG  translated to  GUG

d)

Codon GAG  transcribed to  GTG  translated to  GTG

8.

A protein with molecular weight 12,000 Dalton contains 4 charge. The MS detects the protein ions at

a)

1204

b)

3001

c)

301

d)

12004

9.

A proteolytic enzyme that digest blocks in arteries following myocardial infarction is

a)

CD3

b)

OK-T3

c)

tPA

d)

IL2

10.

Which one of the following has been approved for diabetics who develop skin ulcers.

a)

human growth factor

b)

humulin

c)

platelet derived growth factor

d)

EPO

11.

Regulatory factors like INF,IL,TNF,CSF were initially known as

a)

macrophages

b)

immunoglobulins

c)

cytokines

d)

flavonoids

12.

A component involved in micelle stabilization of milk protein is

a)

beta casein

b)

beta lactam

c)

Kappa casein

d)

kappa lactam

13.

On heating the amino group of proteins reacts with aldehyde group of sugars that gives browned steak its distinctive flavor is by the reaction

a)

Backing reaction

b)

Maillard reaction

c)

Caramelization

d)

Emulsification

14.

Which type of bacteria are extensively used in probiotic drinks.

a)

gram -ve

b)

gram +ve

15.

Gamma glutamyl cysteinyl glycine helps in

a)

detoxification of xenobiotics

b)

protection of cellular components from effect of free radicals.

c)

probiotics

d)

all the above