WorksheetsBiotechnology : Principles and Processes, Quiz 3
Total questions: 10
Worksheet time: 4mins
Isolation of DNA from a fungal cell involves the use of enzyme
Chitinase
Lysozyme
Eco R1
Hind-II
Most commonly used matrix in gel electrophoresis is
ethidium bromide
agarose
polyacrylamide
sulphate bromide.
The process of separation and purification of expressed protein before marketing is called :
Downstream processing
Bioprocessing
Postproduction processing
Upstream processing
What is the criterion for DN A fragments movement on agarose gel during gel electrophoresis ?
The smaller the fragment size, the farther it moves
Negatively charged fragments do not move
The larger the fragment size, the farther it moves
Positively charged fragments move to farther end
For a DNA to function as a cloning vector the most essential requirement is -
multiple restriction sites
several selectable markers
circular nature
'ori' sequence
In PCR, it is _________ that creates single-stranded template molecules
heat
high salt concentration
DNA polymerase
exonuclease
Gene cloning is:
Gene is isolated and inserted in same organism
Gene is isolated and inserted in different organism
Gene is isolated and inserted in plasmid of other organism
Gene is isolated and inserted in chromosomal DNA
The two antibiotic resistance genes on vector pBR322 are for
Tetracycline and Kanamycin
Ampicillin and Tetracycline
Ampicillin and Chloramphenicol
Chloramphenicol and Tetracycline
DNA precipitation out of a mixture of biomolecules can be achieved by treatment with
Isopropanol
Methanol at room temperature
Chilled ethanol
Chilled chloroform
A selectable marker is used to :
Mark a gene on a chromosome for isolation using restriction enzyme
Identify the gene for a desired trait in an alien organism
Help in eliminating the non-transformants, so that the transformants can be regenerated
Select a suitable vector for transformation in a specific crop
