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WorksheetsPRELIM BB
Total questions: 100
Worksheet time: 1hrs 6mins
1. The minimum hemoglobin concentration in a fingerstick from a male donor:
12.0 g/dL
12.5 g/dL
13.5 g/dL
15.0 g/dL
A cause of permanent deferral on blood donation is:
History of therapeutic rabies vaccine
Residence in an endemic malaria region
Diabetes
History of jaundice of uncertain cause
Which of the following prospective donors would be accepted for donation?
32-year old woman who received a transfusion in a complicated delivery 5 months previously
19-year old sailor who has been stateside for 9 months and stopped taking his medication 9 months previously
45-year old woman who has just recovered from a bladder infection and still taking antibiotics.
22-year old college student who has a temperature of 99.2F (37.3C) and state that he feels well, but is nervous about donating
Which one of the following constitute permanent rejection status of a donor?
2 units of blood transfused 4 months previously
Recent close contact with a patient with viral hepatitis
A tattoo 5 moths previously
Confirmed positive test for HBsAg 10 years previously
According to AABB standards, which of the following donors may be accepted as a blood donor?
Received a blood transfusion 22 weeks previously
Traveled to an endemic area for malaria 9 moths previously
Spontaneous abortion 2 months of pregnancy, 3 months previously
Resides with a known hepatitis B patient
Prior to blood donation the intended venipuncture site must be cleaned with a solution containing
Sodium Hypochlorite
10% Bleach
Isopropyl alcohol
PVP iodine complex
All donor blood testing must include
Serological test for syphilis
Direct antiglobulin test
Anti-CMV testing
Complete Rh phenotyping
Rejuvenation of a unit red blood cells is a method used to:
Remove antibody attached to RBCs
Inactivate viruses and bacteria
Restore 2, 3 DPG and ATP normal levels
Filter blood clots and other debris
The loss of ATP leads to:
An increase in phosphorylation of spectrin and a loss of membrane deformability
An increase in phosphorylation of spectrin and an increase of membrane deformability
A decrease in phosphorylation of spectrin and a loss of membrane deformability
A decrease in phosphorylation of spectrin and an increase of membrane deformability
The majority of normal adult hemoglobin consists of:
Two alpha chains and two beta chains
Two alpha chains and two gamma chains
Two alpha chains and two delta chains
Four beta chains
When RBCs are stored, there is a “shift to the left.” This means:
Hemoglobin oxygen affinity increases owing to anincrease in 2,3-DPG
Hemoglobin oxygen affinity increases owing to a decrease in 2,3-DPG
Hemoglobin oxygen affinity decreases owing to adecrease in 2,3-DPG
Hemoglobin oxygen affinity decreases owing to anincrease in 2,3-DPG
Which of the following anticoagulant-preservatives provide a storage time of 21 days at 1–6C for units of whole blood and prepared RBCs if an additive solution is not added?
ACD
CP2D
CPD
All of the above
Additive solutions are approved for blood storage for how many days?
21
42
35
7
A unit of whole blood or packed RBCs in an adult should increase the hematocrit level by _____or the hemoglobin level by _________.
3%, 10-15 g/L
3%, 1.0-1.5 g/L
10-15 g/L, 3%
1.0-1.5 g/L, 3%
It binds ionized calcium and serves as an anticoagulant
Adenine
Glucose
Citrate
Oxalate
How frequent does FDA inspects blood banks?
once a year
twice a year
quarterly
None of the above
This process involve testing if the blood drawn from donor could potentially transmit a disease to recipient
Donor history questionnaire
Physical examination
Donor Screening
Compatibility testing
It Involves withdrawal, processing and reinfusing of blood to the individual simultaneously and does not involve cycles or passes.
Continuous flow centrifugation
Intermittent Flow Centrifugation
Filtration
Filtration and centrifugation
This is used to treat patients who have abnormally elevated platelet counts such as in the case of polycythemia vera.
Neocytopheresis
Leukapheresis
Erythrocytapheresis
Plateletpheresis
Color label which indicates biohazard:
Red
Purple
Gray
Green
Color which may indicate that the blood is not for transfusion.
Gray
Green
Orange
Purple
For apheresis donors who donate platelets more frequently than every 4 weeks, a platelet count must be performed prior to the procedure and be atleast:
150, 000
200, 000
250, 000
300, 000
The most common cause of post-transfusion hepatitis can be detected in donors by testing for:
anti-HBc
anti-HCV
Anti-HAV
HBsAg
The test that is currently used to detect donors who are infected with the AIDS virus is:
anti-HBc
Anti-HTLV1 and II
ALT
Anti-HIV 1 and II
A common screening method for HIV detection is:
Latex agglutination
Radioimmunoassay
Thin-layer chromatography
enzyme linked immunosorbent assay
The optimum storage temperature for Red Blood cells is:
-80 C
-20 C
-12C
4C
What is the minimum pre-transfusion testing requirements for autologous donations collected and transfused by the same facility?
ABO and Rh typing
ABO, Rh type, antidoy screen
ABO/Rh type, antibody screen, crossmatch
No pre-transfusion is required for autologous donations
These individual defined the fourth group AB.
Landsteiner and Wiener
Descatello and Sturli
Landsteiner and Blundell
Jean Baptiste Dennis and Emmerez
SITUATION: A 53-year-old woman donates blood at her place of employment. She weighs 150 lb and has a hemoglobin of 13 g/dL. She is currently on warfarin and vitamin B12. Is she an acceptable donor?
Yes
No, she is on warfarin
Yes, for red cells only
No, her hemoglobin is too low
Which of the following donors could be accepted for whole-blood donation?
A construction worker who was incarcerated for
opiate abuse
A triathlete with a pulse of 45
A man who is currently taking finasteride (Propecia)
A woman in her 14th week of pregnancy
Type of deferral: People who are taking insulin from cows
3 years
1 year
No deferral
Permanent Deferral
A woman begins to breathe rapidly while donating blood. Choose the correct course of action.
Continue the donation; rapid breathing is not a reason to discontinue a donation
Withdraw the needle, raise her feet, and administer ammonia
Discontinue the donation and provide a paper bag
Tell her to sit upright and apply a cold compress to her forehead
Recipient of anthrax vaccine.
No deferral
Permanent deferral
1 month
2 weeks
How much anticoagulant would have to be removed from the collection bag given a donor who weighs 90 lb?
12 mL
15 mL
20 mL
23 mL
Age requirements for autologous donor.
16 years old at least
No age requirements
17 years old to 65 years old atleast
18 years old above
Donors who have ingested/takingAccutane proscar is deferred for ______ starting from the last dose.
Permanently deferred
Not deferred
1 month deferred
1 year deferred
It serves as source of 2,3 diphosphoglycerate (2,3 DPG) which promotes oxygen release to tissues.
Mannitol
Glucose
Saline
Phosphate
He suggested in 1941 that blood groups shall be made the basis for selection of donors for blood transfusion.
Blundell
Denisse
Hektoen
Landsteiner
Whole blood/RBCs can be stored in CPDA-1 at 1C–6C for how many days?
42 days
35 days
21 days
2 days
Those recipient of rubeola vaccine are deferred for:
1 month
2 weeks
No deferral
1 year
It was considered the principal obstacle for blood transfusion during the early times.
Anti-coagulant
Clotting
Lack of transfusion apparatus
Lack of component separation machine
His work on ABO blood grouping won a Nobel Prize in the 20th Century.
Landsteiner
Wiener
Sturli
Susumu Tonegawa
The pre-donation process consists of three (3) steps or processes which includes all of the following except?
Abbreviated Physical Examination
Donor History Questionnaire
Donor Reaction Assessment
Educational Materials
The 10% of ATP needed by the RBC is provided by the ?
Embden-Meyerhoff pathway
Aerobic Pathway
Anaerobic Pathway
Luebebering Pathway
Which of the following does not match:
Karl Landsteiner-ABO Blood Group
Dr. Charles Drew-RH Blood group
Jean Baptiste Denis-Animal to Animal Transfusion
Coombs-Antiglobulin Testing
He determined the minimum amount of citrate needed for anticoagulation and demonstrated its nontoxicity in small amounts.
Dr. Charles Drew
Coombs
Landois
Rous and Turner.
Lewishon
Which of the following ion is more predominant outside than inside the RBC Cell?
Calcium
Potassium
Sodium
Phosphate
A donor bag is half filled during donation when the blood bag is half filled during donation when the blood flow stops. Select the correct course of action.
Closely observe the bag for at least 3 minutes; if blood flow does not resume, withdraw the needle
Remove the needle immediately and discontinue the donation
Check and reposition the needle if necessary; if blood flow does not resume, withdraw the needle
Withdraw the needle and perform a second venipuncture in the other arm
Who is the best candidate for a predeposit autologous donation?
A 45-year-old man who is having elective surgery in 2 weeks; he has alloanti-k
A 23-year-old female leukemia patient with a hemoglobin of 10 g/dL
A 12-year-old boy who has hemophilia
A 53-year-old woman who has septicemia
Recipients of German measles vaccines are deferred for?
2 weeks
Not deferred
1 month
3 months
Autologous donor who is pregnant should be deferred for?
6 months after post-partum
1 year
3 months after post-partum
6 weeks after post-partum
No deferral unless proven risky to the donor.
The gel technology was discovered by Dr. Yves Lapiere in ______.
1980
1985
1890
1895
Which of the following is an acceptable time in which a unit of whole blood is collected?
33 minutes
25 minutes
20 minutes
13 minutes
In blood banking, the commonly used cryoprotective agent that allows freezing of blood for long-term storage is?
Rejuvesol
Citrate
Glycerol
Mannitol
The three areas of RBC biology that are crucial for normal erythrocyte survival and function are the following except?
RBC metabolism
Hemoglobin structure and function
Normal erythropoietin function
Normal chemical composition and structure of the RBC membrane
Main lipid component of the RBC membrane.
Triglycerides
Lipoproteins
Cholesterol
Phospholipids
The RBC zeta potential is maintained by?
Spectrin
Protein Band 4.1
Ankyrin
Glycophorin A
Glycophorin B
RBC viability/shelf-life?
100 days
120 days
110 days
90 days
Major extracellular anion of the RBC membrane?
Bicarbonate
Chloride
Sodium
Potassium
The aerobic/oxidative pathway of RBC metabolism provides reduced glutathione that is important to?
prevent denaturation of hemoglobin
preserve the RBC membrane
promote electrolyte exchange between the ICF and ECF
All of the above
Which hemoglobin determination method is commonly used during blood donation?
POCT
Drabskin Method
Copper Sulfate method
CBC
A specific gravity of 1.053 is equivalent to _______ of hemoglobin.
125 g/L
13.5 g/dL
120 g/L
12.0 g/dL
A 450 unit of whole blood has a standard anticoagulant content of?
70 mL
14 mL
63 mL
60 mL
All of the following apply to a double red cell unit apheresis collection except:
The hematocrit must be at least 38%
The weight for a female is at least 150 lb
The height for a male is at least 5 ft 1 in.
The deferral period following collection is 16 weeks
Serological testing for whole blood donation utilizes:
Yellow stoppered tubes
EDTA tubes
Red tube
Gray tube
What is the right thing to do if if low volume unit is withdrawn and no adjustment done on the volume of anticoagulant?
Accept the unit
Discard the unit
Use the unit as control for blood bank
Donate the unit to other laboratories to be used for creating known A and B cells to prevent wastage
Recommended storage of platelet.
Room temperature
Body temperature
Ref temperature
Any of the above as long as they follow the recommended storage days
Platelet concentrates are prepared using?
heavy spin followed by soft spin/light spin
Soft spin followed by hard spin
Either of the two
hard spin only
Soft spin only
The ABO Blood Group should be printed in?
white
black
Red
Blue
Can an autologous donor donate blood on Monday, if he is having surgery on Friday?
Yes, he or she can donate up to 72 hours before surgery
No, he or she cannot donate within 7 days of surgery
Yes, he or she can donate, but only a half a unit
No, he or she cannot donate within 5 days of surgery
Blood is collected from a drainage tube placed at the surgical site and it is re-infused with or without processing via a microaggregate filter to screen out any debris.
Intra-operative Collection
Post-operative Collection
Acute Normovolemic Hemodilution
Pre-operative Collection
This drug is administered to the donors 12- 24 hours before pheresis to increase the number of circulating granulocytes by pulling them from the marginal pool .
Sedimenting Agent
Corticosteroids
Pooling drug
All of the above
A common sedimenting agent used for granulocyte collection which causes red cells to form rouleaux thus following WBCS to be harvested more efficiently.
Glycerol
Mannitol
hydroxyethyl starch
Corticosteroids
Leukapheresis can be used as therapeutic method for all of the following conditions except?
Cutaneous T cell lymphoma
Acute Myelogeneous Leukemia
Hairy Cell leukemia
Thalassemia
Common replacement fluids used in therapeutic plasmapheresis are:
NSS
NSA
PPF
All of the above
None of the above
To remove the offending agent in the plasma causing symptoms in cases of multiple myeloma and waldenstrommacroglobulinemia, which pheresis method should be performed?
Erythrocytapheresis
Leukapheresis
Therapeutic plasmapheresis
Neocytopheresis
Platelet content of a platelet concentrate obtained via plateletpheresis?
3.0x 1011
5.5 x1010
3.0 x 1010
5.5 x1011
1 unit of platelet concentrate obtained via plateletpheresis is equivalent to how many units of platelet obtained from whole blood?
8-10 units
6-8 units
4-6 units
4-8 units
What is the method used to detect the specific malarial species found on donor's blood?
Thin smear
Thick smear
Both
Either of the two
A male cancer patient with a hemoglobin of 6 g/dL was admitted to the hospital with acute abdominal pain. Small bowel resection was indicated, but the attending physician wanted to raise the patient’s hemoglobin to 12 g/dL before surgery. How many units of RBCs would most likely be required to accomplish this?
2
3
6
8
Which of the following is not a viable method for removing leukocytes from red blood cells?
Pre-storage filtration
Bedside filtration
Post-storage filtration
Buffy coat removal
Four units of packed RBCs were brought to the nurses’ station at 10:20 a.m. Two units were transfused immediately, and 1 unit was transfused at 10:40 a.m. The remaining unit was returned to the blood bank at 11:00 a.m. The units were not refrigerated after leaving the blood bank. What problem (s) is (are) present in this situation?
The only problem is with the returned unit; the 30-minute limit has expired and the unit cannot be used.
The unit should not have been transfused at 10:40 a.m. because the time limit had expired; this unit and the remaining unit should have been returned to the blood bank.
The returned unit may be held for this patient for 48 hours but cannot be used for another patient.
No problems; all actions were performed within the allowable time limits.
Which of the following is acceptable according to AABB standards?
Rejuvenated RBCs may be made within 3 days of outdate and transfused or frozen within 24 hours of rejuvenation
Frozen RBCs must be prepared within 30 minutes of collection and may be used within 10 year
Irradiated RBCs must be treated within 8 hours of collection and transfused within 6 hours
Leukocyte-reduced RBCs must be prepared within 6 hours of collection and transfused within 6 hours of preparation
What is the component of choice for a patient with chronic granulomatous disease (CGD)?
FFP
Granulocytes
Cryoprecipitate
RBCs
All of the following statements regarding FFP are true, except:
FFP must be prepared within 24 hours of collection
After thawing, FFP must be transfused within 24 hours
Storage temperature for FFP with a 1-year shelf life is ≤−18°C
When thawed, FFP must be stored between 1°C–6°C
What may be done to RBCs before transfusion to a patient with cold agglutinin disease in order to reduce the possibility of a transfusion reaction?
Irradiate to prevent graft-versus-host-disease (GVHD)
Wash with 0.9% percent saline
Warm to 37°C with a blood warmer
Transport so that temperature is maintained at 20°C–24°C
A unit of packed RBCs is split using the open system. One of the half units is used. What may be done with the second half unit?
Must be issued within 24 hours
Must be issued within 48 hours
Must be irradiated
Must retain the original expiration date
What should be done if a noticeable clot is found in an RBC unit?
Issue the unit; the blood will be filtered
Issue the unit; note the presence of a clot on the release form
Filter the unit in the blood bank before issue
Do not issue the unit
Which of the following component(s) is (are) indicated or appropriate for patients who have anti-IgA antibodies?
Whole blood
Packed RBCs
Washed or deglycerolized RBC
Granulocytes
FFP can be transfused without regard for:
ABO type
Rh type
Antibody in product
All of these options
Which of the following is true regarding granulocyte concentrates?
The product must contain a maximum of 1.0 × 1010 granulocytes
The pH must be 6.0
The product must be crossmatched
The product must be irradiated
What course of action should be taken if a medical laboratory scientist?
Issue the unit
Discard the unit
Change the expiration date; then issue the unit
Note on the irradiation sticker that the unit was irradiated twice and issue
What components(s) may be shipped together with FFP?
Frozen RBCs and cryoprecipitate
Platelets
Packed RBCs and granulocytes
Double red cell
A blood supplier ships 3 units of pooled cryoprecipitate. Each pool consists of 5 units of cryoprecipitate. If one unit is thawed at 5:00 p.m., when must it be dispersed from the blood bank?
Before 9:00 p.m.
Before 11:00 p.m.
Before 12:00 a.m.
Before 5:00 p.m. the next day
All of the following are true regarding washed RBCs, except:
RBCs are washed with 1–2 L of normal saline
Volume is 180 mL
Shelf life is extended
Leukocytes are removed
All of the following are advantages of using single donor platelets as opposed to random donor platelets, except:
Less preparation time
Less antigen exposure for patients
May be HLA matched
No pooling is required
SITUATION: A cancer patient recently developed a severe infection. The patient’s hemoglobin is 8 g/dL owing to chemotherapy with a drug known to cause bone marrow depression and immunodeficiency. Which blood products are indicated for this patient?
Liquid plasma and cryoprecipitate
Crossmatched platelets and washed RBCs
Factor IX concentrates and FFP
Irradiated RBCs, platelets, and granulocytes
Which of the following is true regarding apheresis platelets?
The minimum platelet count must be 3.0 × 1011, pH must be ≥6.0
The minimum platelet count must be 3.0 × 1010, pH must be ≤6.2
The minimum platelet count must be 3.0 × 1011, pH must be ≥6.2
The minimum platelet count must be 5.5 × 1010,
pH must be ≤6.0
A unit of whole blood is collected at 10:00 a.m. and stored at 20°C–24°C. What is the last hour platelet concentrates may be made from this unit?
4:00 p.m
6:00 p.m
7:00 p.m
8:00 p.m
How does irradiation prevent transfusion associated graft-versus-host disease (TA-GVHD)?
Gamma rays and x-rays destroy the lymphocytes’ ability to divide
X-rays cause lysis of the lymphocytes
Gamma rays enhance lymphocyte reactivity
Ultraviolet radiation induces apoptosis of lymphocytes
