Genera of Flaviviridae

→ Hepatitis C

→ Has no arthropod vector (therefore, hindi siya Arbovirus)

Genera of Flaviviridae

→ No known human pathogens

→ Includes animal pathogens (e.g., bovine viral diarrhea and hog cholera viruses) that are of considerable economic importance

Pathogenic Flavivirus

The ff. is the classification of diseases based on their clinical manifestation. Identify the symptom/syndrome based on the ff. diseases

• St. Louis Encephalitis (SLE)

• Japanese Encephalitis (JE)

• Murray Valley Encephalitis/ Kunjin Rocio (MVE)

• Tick-borne Encephalitis (TBE)

Pathogenic Flavivirus

The ff. is the classification of diseases based on their clinical manifestation. Identify the symptom/syndrome based on the ff. diseases

• West Nile (WN)

• Chikungunya (KUN)

• Dengue Virus (DEN)

Pathogenic Flavivirus

The ff. is the classification of diseases based on their clinical manifestation. Identify the symptom/syndrome based on the ff. diseases

• Omsk Hemorrhagic Fever

• Kyasanur Forest Disease Virus

• Yellow Fever (YF)

• Dengue Virus (DEN)

Properties & Structures

Have __________ genome, icosahedral capsid, and an envelope. It is also inactivated by acid pH, bleach, heat, phenol, lipid solvents, 70% alcohol, detergents and formaldehyde

Properties & Structures

Most pathogenic flaviviruses are antigenically related (antibodies to one virus may neutralize another virus) and they share same antigenic sites

Properties & Structures

_______ (E) protein exhibits hemagglutinating activity & contains group-, serocomplex-, and type-specific determinants

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Genome & Replication (Genome part)

A large precursor protein is produced from genome-length _____ during viral replication and upon translation, several enzymes cut the single polypeptide into functional protein units

Genome & Replication (Genome part)

Structural proteins are encoded at the 5’ end of the genome

Genome & Replication (Genome part)

Structural proteins that is highly basic component of nucleocapsid

Genome & Replication (Genome part)

Structural proteins that is precursor of M protein

Genome & Replication (Genome part)

Structural proteins that is membrane-associated and serve a matrix function, linking capsid and envelope

Genome & Replication (Genome part)

Structural proteins that is major envelope protein; virion assembly, receptor binding and membrane fusion

Genome & Replication (Genome part)

Non-structural protein that is a membrane soluble hemagglutinin

Genome & Replication (Genome part)

Non-structural protein that is a protease or polymerase

Genome & Replication (Genome part)

Non-structural protein that is a polymerase

Genome & Replication (Genome part)

Non-structural protein that works in tandem with NS3 as a protease

Genome & Replication (Replication part)

Replicate in the ______ (20–30 hours), and particle assembly occurs in _______ vesicles (in association with Golgi or smooth membranes)

Genome & Replication (Replication part)

The viruses acquire their envelope by budding into the _______

Genome & Replication (Replication part)

Characteristic of infected cells is proliferation of extracellular membranes

Genome & Replication (Replication part)

Genomic RNA is capped (not polyadenylated) and serves as mRNA for all proteins and release through cell lysis

Genome & Replication (Replication part)

This is a viral molecule involved in the replication that is:

• Made from genomic RNA by NS proteins

• Serves as a template for progeny genomic RNA

Genome & Replication (Replication part)

This is a viral molecule involved in the replication that is:

• Encoded at the 5’ end of the genome → Synthesized first and with greatest efficiency and also allows production of more structural proteins, but decreases the efficiency of nonstructural protein synthesis and initiation of viral replication

• Contribute to the lag before detection of their replication

Genome & Replication (Replication part)

This is a viral molecule involved in the replication that is:

• Encoded in the 3' two-thirds

• E.g. NS-1 and RNA-dependent RNA polymerase

Pathogenesis

Primary viral multiplication occurs in either:

Pathogenesis

Ultimate nature of disease is determined by the following EXCEPT:

Pathogenesis

Process of human infection:

1. Virus replicates in local tissues & regional lymph nodes

2. Initial viremia

3. Deposition of virus through the skin via the saliva of an

infected female arthropod

4. Infection resolves and lasting immunity is produced

5. Secondary viremia

Pathogenesis

Initial viremia starts within 3-7 days of infection and produces systemic symptoms (fever, chills, headaches, etc.)

What is the primary target cells of flaviviruses?

Clinical Manifestations

Most infections with St. Louis encephalitis (SLE) and JE viruses have either no apparent disease or a nonspecific febrile illness with headache

Clinical Manifestations

CNS invasion may develop and may result to aseptic meningitis or encephalitis

→ Incubation period of the encephalitides: bet. 4 and 21 days

→ Sudden onset with severe headache, chills and fever, nausea and vomiting, generalized pains, malaise

→ Within 24-48 hours, there is marked drowsiness and the patient may become stuporous.

→ Mental confusion, tremors, convulsions, and coma develop in severe cases.

→ Fever lasts 4-10 days

→ Sequelae may be mild to severe and include mental

deterioration, personality changes, paralysis, aphasia, and cerebellar sign.

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