WorksheetsSpecial Collections and Point-of-care Testing
Total questions: 52
Worksheet time: 26mins
Non-routine laboratory tests that may not only involve additional preparation and procedure but may require other specimens such as urine or feces. These can be applied for special cases such as blood donation.
Special Palamig
Special Halo-halo
Special Collection Procedures
Cross-match Test
Important in determining which blood product can be safely used for blood transfusion.
Blood Bank Specimen
Blood Culture Testing
Coagulation Specimen
Glucose Test
These are all blood bank identification system, EXCEPT:
ID bracelet
Blood ID band
Siemens patient identification check-blood administration
School ID
Tube color/additive for used for the collection of blood bank specimen.
Lavender Top or EDTA tube
Light Blue Top or Sodium Citrate tube
Yellow Top or SPS tube
Gray Top or Potassium Oxalate and Sodium Fluoride tube
Blood Donors should not be/have:
17 to 66 years of age
Minimum weight of 110 lbs or 50 kg
Failed physical examination
A test that checks the blood for pathogens for patients who have a fever of unknown origin (FUO). The test determines the presence of bacteria in the blood that causes bacteremia or presence of microorganism and toxins in the blood that causes septicemia.
Blood Bank Specimen
Blood Culture Testing
Coagulation Specimen
Glucose Test
How many blood culture sets should we collect?
5-6 blood culture sets
1-2 blood culture sets
4-6 blood culture sets
2-4 blood culture sets
Crucial in the blood collection procedure because it prevents the contamination by microbial flora in the skin which can be introduced in blood culture bottles and affect the results.
Skin Antisepsis
Skin Testing
Hemostasis
Vasoconstriction
Also called Normal Flora, are the microorganisms that live on other living organisms or inanimate objects without causing disease.
Microbacterial Flora
Microbial Flora
Microbium Flora
Microtainer Flora
Antiseptics used in blood culture: choose two (2)
Benzalkonium Chloride
Chlorhexidine Gluconate
Bleach
Hydrochloric Acid
How many seconds should you perform friction rub at a puncture site?
10-20 seconds
15-30 seconds
20-30 seconds
30-60 seconds
True or False: In blood culture testing, to mix the blood with the medium, shake the bottle a couple of times.
True
False
3 ways to inoculate the medium, EXCEPT:
Direct Inoculation
Syringe Method
Intermediate Collection Tube
Adhesion
A butterfly and a specially designed holder are used. The holder is connected to the Luer connector of the butterfly collection set.
Direct Inoculation
Syringe Method
Intermediate Collection Tube
Blood is transferred to the culture bottles after completing the draw using a safety transfer device, which is activated as soon as the needle is removed from the site.
Direct Inoculation
Syringe Method
Intermediate Collection Tube
Performed in the laboratory rather than the patient’s bedside. Nonetheless, this is not recommended because the sodium polyanethole sulfonate (SPS) in collection tubes increases its concentration when added to the blood culture bottles.
Direct Inoculation
Syringe Method
Intermediate Collection Tube
A physician requests this to assess the blood clotting functions especially if the patient has unexplained bleeding.
Blood Banking
Blood Culture Testing
Coagulation Tests
Hemostasis
All are coagulation tests, EXCEPT:
Prothrombin Time (PT)
Activated Partial Thromboplastin Time (APTT)
Thrombin Time (TT)
Glucose Tolerance Test (GTT)
A complex process in which multiple components of the blood clotting system are activated in response to vessel injury to control bleeding.
Hemostasis
Homeostasis
Vasoconstriction and platelet plug formation. The key component of primary hemostasis is the platelet. Primary Hemostasis is triggered by injury to the vessel wall, exposing subendothelial collagen.
Primary Hemostasis
Secondary Hemostasis
Fibrin Clot formation and stabilization
Inhibition of Coagulation
Activation of coagulation factors and the generation of thrombin.
Primary Hemostasis
Secondary Hemostasis
Fibrin Clot formation and stabilization
Inhibition of Coagulation
Thrombin converts fibrinogen to fibrin monomers which polymerize to form a soluble clot. Thrombin will activate factor XIII which cross-links to fibrin monomers and will stabilize the clot.
Primary Hemostasis
Secondary Hemostasis
Fibrin clot formation and stabilization
Inhibition of Coagulation
Inhibition of thrombin generation and fibrin clot breakdown (fibrinolysis). Once the vessel is repaired the clot must be dissolved to restore the blood flow.
Primary Hemostasis
Secondary Hemostasis
Fibrin clot formation and stabilization
Inhibition of Coagulation
Factors III, VII
Extrinsic Pathway
Intrinsic Pathway
Common Pathway
Factors X, V, II, Fibrinogen
Extrinsic Pathway
Intrinsic Pathway
Common Pathway
Factors XII, XI, IX, VIII
Extrinsic Pathway
Intrinsic Pathway
Common Pathway
Used to assess deficiencies or inhibitors of the Extrinsic pathway factors (factor III, VII) and Common pathway factors (X, V, II, fibrinogen). Measures how long it takes for a clot to form in a blood sample.
Prothrombin Time (PT)
Activated Partial Thromboplastin Time (APTT)
Thrombin Time (TT)
Used to assess deficiencies or inhibitors of the Intrinsic pathway factors (XII, XI, IX, VIII) and Common pathway factors (X, V, II, fibrinogen). Measuring a person’s ability to form blood clots.
Prothrombin Time (PT)
Activated Partial Thromboplastin Time (APTT)
Thrombin Time (TT)
Used to assess deficiencies or dysfunction of fibrinogen or the presence of an inhibitor of thrombin (factor IIa)
Prothrombin Time (PT)
Activated Partial Thromboplastin Time (APTT)
Thrombin Time (TT)
The process in which fibrin is dissolved. Produce plasmin, and that plasmin will cleave the fibrin and dissolves the clot.
Fibrinolysis
INR
Prothrombin
Warfarin
Measures prothrombin time. A type of calculation based on prothrombin time test results.
Fibrinolysis
INR
Prothrombin
Warfarin
It is a protein made by the liver, it is a clotting factor.
Fibrinolysis
INR
Prothrombin
Warfarin
A blood-thinning medicine that is used to prevent dangerous clots. It is an anticoagulant therapy.
Fibrinolysis
INR
Prothrombin
Warfarin
Tube color/additive for coagulation specimens
Lavender top or EDTA tube
Light blue top with 3.4% Citrate tube
Light blue top with 3.2% Citrate tube
Pink top or EDTA tube
Blood to coagulant ratio:
9:2
9:1
9:3
9:4
This blood test is done to check if the patient is suffering from diabetes and other metabolic problems. It is also used to monitor insulin therapy.
2-hour Postprandial Glucose
Glucose Tolerance Test
Lactose Tolerance Test
Paternity/Parentage Testing
Also called “Oral Glucose Tolerance Test”; This is to evaluate the ability of the body to metabolize glucose by measuring the tolerance level to high glucose level.
2-hour Postprandial Glucose
Glucose Tolerance Test
Lactose Tolerance Test
Paternity/Parentage Testing
Glucose Tolerance Test Procedure, EXCEPT:
Eat a balanced meal containing approximately 250 grams of carbohydrates for 3 days
Must fast for 12 to 16 hours before the scheduled test
Drinking water is allowed to avoid dehydration
Record the time when the drink was finished then start timing the test which is collected within 30 minutes, 1 hour, 2 hours, and so forth.
It is any degree of glucose intolerance with onset or first recognition during pregnancy.
Gestational Diabetes Mellitus
Diabetes Mellitus
Measures the ability of the body to process lactose and determines if the patient lacks mucosal lactase which is an enzyme that converts lactose into glucose or galactose.
2-hour Postprandial Glucose
Glucose Tolerance Test
Lactose Tolerance Test
Paternity/Parentage Testing
Performed to exclude the possibility of paternity of a particular child. Before the advent of DNA parenting testing, testing for parentage involves ABO and Rh typing and basic red cell antigen testing.
2-hour Postprandial Glucose
Glucose Tolerance Test
Lactose Tolerance Test
Paternity/Parentage Testing
Measures drug levels at designated intervals so that the appropriate dosage can be established and maintained for the patient, thus avoiding toxicity.
Therapeutic Drug Monitoring
Therapeutic Phlebotomy
Toxicology Test
Forensics Specimen
Performed by drawing a large volume of blood about 500 mL from the patient as part of the treatment procedure for polycythemia and hemochromatosis.
Therapeutic Drug Monitoring
Therapeutic Phlebotomy
Toxicology Test
Forensics Specimen
Tube color/additive used for Therapeutic Drug Monitoring
Lavender Top or EDTA tube
Light Blue Top or Sodium Citrate
Green Top or Heparinized tube
Gray Top or Oxalated tube
The overproduction of red blood cells which is harmful to the patient.
Polycythemia
Anemia
Aminoglycoside Drugs EXCEPT:
Amikacin
Gentamicin
Tobramycin
Alaxin
Involves the collection of blood, hair, urine, and other substances from the body for the purpose of determining the presence of toxins which could be in very small amounts.
Therapeutic Drug Monitoring
Therapeutic Phlebotomy
Toxicology Test
Forensics Specimen
The most common specimens are breath and blood for alcohol. Others request urine for drug screening, blood specimen for drugs, and DNA analysis.
Therapeutic Drug Monitoring
Therapeutic Phlebotomy
Toxicology Test
Forensics Specimen
Usually ordered for purposes related to treatment, but could also be for industrial or job-related purposes such as insurance claims or programs and employee drug screening. The law enforcement department orders Blood Alcohol Concentration (BAC) for individuals involved in traffic-related accidents.
Blood Alcohol (Ethanol) Specimens
Trace Element Testing
Drug Screening
Tube color/additive used for Blood Alcohol (ETOH) Specimens
Lavender Top or EDTA tube
Light Blue Top or Sodium Citrate
Green Top or Heparinized tube
Gray Top or Potassium Oxalate and Sodium Fluoride
The specimen used is urine instead of blood. The chain of custody protocol is strictly implemented since legal implications are involved.
Blood Alcohol (Ethanol) Specimens
Trace Element Testing
Drug Screening
Checks for the presence of aluminum, arsenic, copper, lead, iron, and zinc. They are collected in small amounts and must use special element-free tubes colored royal blue, since traces of these elements in the glass, plastic or stopper could trickle into the specimen will affect the accuracy of the result.
Blood Alcohol (Ethanol) Specimens
Trace Element Testing
Drug Screening
