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Chromatography

Total questions: 100

Worksheet time: 58mins

Name
Class
Date
1.
The physical separation of mixtures into individual components is..
a)
chromatography
b)
opacity 
c)
latent
d)
trace evidence 
2.
Two or more substances that are mixed together but not chemically combined is a ___
a)
compound
b)
mixture
c)
keratin
d)
chromatography
3.
Two or more substances that are chemically combined is a ___
a)
compound
b)
mixture
c)
solvent
d)
solute
4.
chromatography separates the mixture of dyes on the basis of their ----------------------------
a)
density
b)
solubility
c)
gravity
d)
boiling point
5.
On the chromatogram given -------------- is mixture.
a)
red
b)
orange
c)
green
d)
black
6.
A pure substance shows ------------------ spot on chromat gram
a)
0
b)
1
c)
2
d)
3
7.
The diagram below shows a chromatogram obtained when a sample X was analysed together with four other known dyes P, Q, R and S. Dye Q was known to cause cancer. Which of the following dye(s) is/are safe for use?
a)
P only
b)
S only
c)
P and S
d)
P and X
8.
In Chromatography the retardation factor Rf are used for identification of components. A high  Rf and a low Rt (retention time) mean the component has a 
a)
greater affinity to the stationary phase in both TLC and HPLC.
b)
greater affinity to the stationary phase in TLC but a greater affinity for the mobile phase in HPLC
c)
greater affinity to the mobile phase in TLC but a greater affininty for the stationary phase in HPLC
d)
greater affinity to the mobile phase in  both TLC  and HPLC
9.
In Chromatography the retardation factor Rf are used for identification of components. A low  Rf and a high Rt (retention time) mean the component has a 
a)
greater affinity to the stationary phase in both TLC and HPLC.
b)
greater affinity to the stationary phase in TLC but a greater affinity for the mobile phase in HPLC
c)
greater affinity to the mobile phase in TLC but a greater affininty for the stationary phase in HPLC
d)
greater affinity to the mobile phase in  both TLC  and HPLC
10.
A high Rf value indicates strong
a)
strong affinity to the stationary phase
b)
strong affinity to the mobile phase
c)
no affinity to the stationary phase
d)
 no affinity to the stationary phase
11.
A chromatography experiment uses water as a mobile phase. Which of the following would have the highest Rf value
a)
ethanol
b)
propanol
c)
butanol
d)
pentanol
12.
A low retention time indicates
a)
a high affinity to the mobile phase
b)
a low affinity to the stationary phase
c)
a high solubility in the mobile phase
d)
all of the above
13.

A substance is soluble if it can be dissolved.


In paper chromatography, the most soluble solute _____________________ .

a)

does not move from the start point

b)

stays closest to the start point

c)

travels furthest away from the start point

d)

can be found in the middle of the chromatogram

14.

Which of the following mixture/s is/are separated by chromatography?

a)

Pigments from natural colours

b)

Colours in a dye

c)

Colours of flowers extracts

d)

All the above

15.

Which of the following is correct about chromatography?

a)

It separates those solutes that dissolve in the same solvent.

b)

It separates those solutes that dissolve in different solvents.

c)

It separates a solute that dissolves in a solid solvent.

d)

It separates gaseous solutes from liquid solvents.

16.
Why is Chromatography useful?
a)
It can be used as medicine.
b)
It can be used for finding clues at a crime scene
c)
It can be used to make bombs.
d)
It can reduce the lack of food and water.
17.

If a substance is not soluble in the solvent, what happens to the spot placed on the start line?

a)

a) Dot moves to the top of the paper

b)

b) Dot does not move at all

c)

c) Dot moves to the left or right

d)

d) Dot moves down to the bottom of the paper

18.

In size exclusion chromatography, the higher the degree of cross-linking of the gel polymer, ………..

a)

the lower the mechanical strength and the larger the molecular sieving range.

b)

the higher the mechanical strength and the larger the molecular sieving range.

c)

the lower the mechanical strength and the smaller the molecular sieving range.

d)

the higher the mechanical strength and the smaller the molecular sieving range.

19.

In gel permeation chromatography, a type of size exclusion C, which of the following is correct?

a)

Compressible gels are commonly used.

b)

Spherical bead shape is preferred.

c)

Mechanical strength increases with large particle size.

d)

Gels with large bead size are preferred.

20.

Q. Chromatography is generally used for the separation of mixtures based on the differences in their

a)

shape

b)

size

c)

structure

d)

all of the above

21.

Ion-exchange chromatography is used for the separation of

a)

polar molecules

b)

nonpolar molecules

c)

none of the above

d)

all of the above

22.

Which chromatographic technique depends on the highly specific interactions between pairs of biological materials such as enzyme-substrate?

a)

ion exchange chromatography

b)

affinity chromatography

c)

adsorption chromatography

d)

igel permeation chromatography

23.

In gel filtration (size exclusions) chromatography, separation of proteins are based on their

a)

size and net charge

b)

size and shape

c)

size and specific affinity

d)

shape and net charge

24.

Which polymer is often used for matrix materials in Affinity chromatography

a)

Agarose

b)

cellulose

c)

dextrose

d)

all of the above

25.

In anion exchange chromatography,

a)

the column contains positively charged beads where negatively charged proteins bind

b)

the column contains negatively charged beads where positively charged proteins bind

c)

the column contains negatively & positively charged beads where proteins bind depending on their net charge

d)

all of the above

26.

Chromatography is used to separate the components of a mixture and can be carried out in a range of different ways.

a) A suitable example of a ‘carrier gas’ in gas chromatography is

a)

Chlorine

b)

Nitrogen

c)

Steam

d)

Not possible to state, as there should be a vacuum

27.

(b) Separation is achieved in gas chromatography due to the components in the mixture having different

a)

Interactions with the stationary phase.

b)

Interactions with the mobile phase.

c)

Colours

d)

Solubility in the moving solvent.

28.

Which gas is the least suitable as a carrier gas in Gas-Liquid Chromatography?

a)

Argon

b)

Carbon Dioxide

c)

Oxygen

d)

Nitrogen

29.

Chromatography may be used to separate mixtures of amino acids. This is best explained by the fact that different amino acids have different interactions with

a)

the mobile phase only

b)

the stationary phase only

c)

the mobile phase and the stationary phase

d)

ninhydrin

30.

In one type of HPLC, the stationary phase is non-polar and a polar solvent is used as the eluent. Which of the following would travel through the chromatography column most quickly?

a)

Tetrachloromethane

b)

Chloromethane

c)

Iodomethane

d)

Hexane

31.

In HPLC (High Performance Liquid Chromatography) which of these factors does not affect the time taken for a component to pass through the column?

a)

Type of detector

b)

Material of stationary phase

c)

Particle size of stationary phase

d)

Temperature of column

32.

In gas chromatography, the time taken for a substance to travel through the column is called

a)

Reference time

b)

Retention time

c)

Separation time

d)

Travelling time

33.

Why is GC-MS an important analytical technique in science?

a)

Allows unknown substances to be identified

b)

Quantifies the amount of known chemicals

c)

Characterizes the boiling point of a compound

d)

Determines the volatility of a compound

34.

Fill in the blank : The more soluble the colour in water __________ the colour is carried

a)

The further up the paper

b)

the more water will soak up the paper

c)

more spots of mixture are placed on pencil line

d)

The lower down the paper

35.

Which of the following columns are not used in HPLC

a)

Analytical column

b)

Separation column

c)

Guard column

d)

Capillary column

36.

Which of the following will improve the efficiency of separation process in liquid chromatography?

a)

Increase in sample size, increase in column diameter

b)

Reduction in sample size, increase in column diameter

c)

Increase in sample size, reduction in column diameter

d)

Reduction in sample size, reduction in column diameter

37.

The retention factor is used

a)

to zero the chromatography scale

b)

to identify unknown compounds in a sample

c)

to retain solvent

d)

as a starting tool for chromatography

38.

Thin layer chromatography is

a)

partition chromatography

b)

electrical mobility of ionic species

c)

adsorption chromatography

d)

none of the above

39.

Ion-Exchange chromatography:

a)

separate and analyze compounds that can be vaporized without decomposition

b)

Also known as Liquid-Solid Chromatography

c)

Separation based on partition coefficient between 2 immiscible liquids

d)

Materials used are either cation or anion resins

40.

The presence of more than one spot on the TLC plate shows that____

a)

the Rf value is higher than it should be

b)

the solvent used was too dilute

c)

impurities are present

d)

too much solvent was used

41.

The Rf value for the red component is

a)

0.3

b)

0.7

c)

10

d)

7

42.

In size exclusion chromatography, the higher the degree of cross-linking of the gel polymer, ………..

a)

the lower the mechanical strength and the larger the molecular sieving range.

b)

the higher the mechanical strength and the larger the molecular sieving range.

c)

the lower the mechanical strength and the smaller the molecular sieving range.

d)

the higher the mechanical strength and the smaller the molecular sieving range.

43.

The stationary phase in this experiment is...

a)

chromatography paper

b)

water

c)

beaker

d)

dye

44.

The mixture on the left contains...

a)

All three dyes

b)

None of the dyes

c)

Dye 1 and Dye 2

d)

Dye 1 and Dye 3

e)

Dye 2 and Dye 3

45.

The dye with the HIGHEST Rf value will be...

a)

Dye 1

b)

Dye 2

c)

Dye 3

d)

All have the same Rf value

46.
Two or more substances that are mixed together but not chemically combined is a ___
a)
compound
b)
mixture
c)
keratin
d)
chromatography
47.
Two or more substances that are chemically combined is a ___
a)
compound
b)
mixture
c)
solvent
d)
solute
48.

Chromatography separates a mixture of dyes on the basis of their

a)

density or weight

b)

gravity

c)

boiling point

49.
Which process will NOT yield a positive identity of a substance?
a)
Electrophoresis
b)
TLC
c)
Infrared Spec
d)
GC with MS
50.
Which instrument is used to measure and record the absorption spectrum of a chemical substance?
a)
Laser
b)
TLC
c)
Mass spectrograph
d)
Spectrophotometer
51.
Separates gaseous substances in a calibrated column into different components
a)
Thin Layer Chromatography
b)
HPLC
c)
Gas Chromatography
d)
Mass Specroscopy
52.
Which forms of Chromatography use Rt values?
I paper chromatoraphy
II TLC
III HPLC
IV GC
a)
I and II
b)
III and IV
c)
II only
d)
III only
53.
Which forms of Chromatography give qualitative and quantitative results?
I paper chromatoraphy
II TLC
III HPLC
IV GC
a)
I and II
b)
III and IV
c)
II only
d)
III only
54.
When using water as a solvent which of these molecules is likely  to have the highest  Rf value
a)
ethanol
b)
ethanoic acid
c)
ethane
d)
ethene
55.
In HPLC  with a polar solvent which components are eluted first
a)
large polar molecules
b)
small non polar molecules
c)
small polar molecules
d)
large non polar molecules
56.
Which of the following can be used to find the concentration of a substance in HPLC
a)
Rf value
b)
Rt value
c)
peak area
d)
peak width
57.
Which  of the following would give greater resolution in GC and HPLC
a)
increased temperature
b)
longer column
c)
fast flow rate
d)
increased pressure
58.
What two properties affect the Rt of a compound?
I Size/weight
II Polarity
III melting point
a)
I only
b)
II only
c)
III only
d)
I and II
59.

In case of chromatography the solvent rises up due to ……………………..

a)

Solute

b)

Filter paper

c)

Capillary action

d)

None of the above

60.

Which of the following cannot be separated by chromatography?

a)

Components of blood

b)

Salt from water

c)

Components of ink

d)

Both a and b

61.

Black dye separates into:

a)

Yellow, red and green colour

b)

Blue, red and yellow colour

c)

Yellow and green colour

d)

Red and yellow colour

62.

Which of the following statements is correct?

a)

The most soluble component remains near the bottom.

b)

The most soluble component rises to the top.

c)

The least soluble component remains near the bottom.

d)

Both b and c

63.

What molecules move up the paper to carry the different colors of ink?

a)

color

b)

fat

c)

water

64.
In chromatography, if a solute does not separate and remains on the start line ....
a)
the solute is insoluble in the solvent
b)
the solute is soluble in the solvent
c)
the solvent is insoluble in the solute
d)
the solvent is soluble in the solute
65.
Whose blood was found at the crime scene?
a)
John
b)
Lisa
c)
Bob
d)
Sure
66.

What type of chromatography is depicted by the illustration?

a)

Gel Filtration Chromatography

b)

Column Chromatography

c)

HPLC

d)

Thin Layer Chromatography

67.

Which of the following methods could be used to check the molecular weight of your purified protein?

a)

SDS-PAGE

b)

Mass Spectrometry

c)

Analytical SEC

d)

All of above

68.

To elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate?

a)

Low salt concentrations

b)

High salt concentrations

c)

Adding a soluble ligand which competes with the affinity tagged protein for binding to the column

d)

Just keep washing buffer through the column, isocratic elution

69.

What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?

a)

Prediction of isoelectric point (pI) from the amino acid sequence

b)

Test protein binding to an IEX matrix at a range of pHs and salt concentrations

c)

Test protein binding to a selection of anion and cation exchange matrices

d)

Pass your sample through a preparative column and elute with a salt gradient

70.

Which of these techniques is often considered a suitable "polishing" step in a protein purification strategy?

a)

Affinity chromatography (AC)

b)

Ion-exchange chromatography (IEX)

c)

Hydrophobic interaction chromatography (HIC)

d)

Size-exclusion chromatography (SEC)

71.

You find that your protein sample loses activity during storage. What can you do about this?

a)

Add an additional purification step

b)

Use a protease inhibitor during purification steps

c)

Perform each step as quickly as possible, in a cold-room

d)

All of the above

72.
The diagram shows the chromatogram for three colouring dyes and that for a brown marker. What dye(s) are contained in the marker's ink?
a)
purple and green
b)
purple and yellow
c)
purple only
d)
green and yellow
73.
The Rf value  for the blue component is 
a)
0.3
b)
0.7
c)
10
d)
3
74.
If a dye is insoluble in water and cannot be separated using chromatography, how can it be tested to see if it is a mixture?
a)
Use filtration instead
b)
Repeat the chromatography test using a different solvent such as propanone
c)
Use distillation instead
d)
Repeat the chromatography using water as the solvent and see if it separates this time
75.

Investigators use ________ chromatography when identifying unknown plant pigments and other compounds.

a)

thin-layer

b)

gas

c)

paper

d)

liquid

76.

____________ Chromatography uses thin plastic or glass trays to identify the composition of pigments, chemicals, and other unknown substances.

a)

Gas

b)

Thin-layer

c)

Liquid

d)

Paper

77.

____________ Chromatography is used to determine the chemical composition of unknown substances, such as the different compounds in gasoline.

a)

Paper

b)

Liquid

c)

Gas

d)

Thin-Layer

78.

____________ Chromatography can be used to separate the components of inks, dyes, plant compounds, make-up and other substances.

a)

Paper

b)

Liquid

c)

Gas

d)

Thin-Layer

79.

__________ is a substance that does dissolve in water.

a)

Solvent

b)

Insoluble

c)

Soluble

d)

Solution

80.

Which colour is the most soluble?

a)

Blue

b)

Green

c)

Yellow

81.

How many spots will show on a chromatogram of a pure substance?

a)

0

b)

1

c)

2

d)

3

82.

What information can you obtain from a chromatogram about the composition of a coloured substance?

a)

whether it contains salt

b)

whether it is pure or a mixture

c)

whether it contains water

d)

whether it has a low boiling point

83.

What information can you obtain from a chromatogram about the composition of a coloured substance?

a)

whether it contains salt

b)

whether it is pure or a mixture

c)

whether it contains water

d)

whether it has a low boiling point

84.

Which separation technique is used to obtain pure water from seawater?

a)

Simple distillation

b)

Filtration

c)

Chromatography

d)

Evaporation

85.

What is the physical property on which fractional distillation depends?

a)

different boiling points

b)

different melting points

c)

different solubilities

d)

different densities

86.

Name the separation method that causes wet clothes on a laundry line to dry

a)

Chromatography

b)

Filtration

c)

Distillation

d)

Evaporation

87.

Hydrophobic molecules can be separated by ion exchange chromatography

a)

True

b)

False

88.

Cationic exchanger is used when molecules of interest is

a)

Positively charged

b)

Negatively charged

c)

Neutral

d)

Zwitterion

89.

Anionic exchanger is used to separate molecules having

a)

Positively charged

b)

Negatively charged

c)

Neutral

90.

Elution can be done by how many types?

a)

1

b)

2

c)

3

d)

4

91.

Elution of anionic exchanger resin can be done by treatment with

a)

Acid

b)

Buffer

c)

NaOH

d)

Ammonia

92.

HPLC stands for

a)

High Performance Layer Chromatography

b)

High Performance Liquid Chromatography

c)

Highly Placed Liquid Chromatography

d)

Hard Performance Liquid Chromatography

93.

HPLC methods include

a)

liquid/liquid (partition) chromatography

b)

liquid/solid (adsorption) chromatography

c)

ion exchange nd size exclusion chromatography

d)

all of the option correct

94.
The substance in the mixture that has the greatest affinity for the mobile phase will travel
a)
fastest
b)
slowest
95.

Based on a normal chromatography, the stationary phase is polar.

a)

TRUE

b)

FALSE

c)

Depends on the substance

96.

A wave with a long wavelength will have a ______ frequency and _____ energy

a)

high, low

b)

high, high

c)

low, high

d)

low, low

97.

A hydrophobic compound will preferentially partition into an aqueous solvent. True or false?

a)

True

b)

False

98.

Chromatography is a physical method that is used to separate and analyse _____________.

a)

Pure compounds

b)

Complex mixtures

c)

Viscous mixtures

d)

Metals

99.

In which type of chromatography, the stationary phase held in a narrow glass tube and the mobile phase is forced through it under pressure?

a)

Column chromatography

b)

Planar chromatography

c)

Liquid chromatography

d)

Gas chromatography

100.

In chromatography, which of the following can the mobile phase be made of?

a)

Solid or liquid

b)

Liquid or gas

c)

Gas only

d)

Liquid only

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