NEW
Font size
WorksheetsTissue Engineering Module 1 MIT-Bio/ Cell Culture Basics
Total questions: 10
Worksheet time: 5mins
The type of culture which is prepared by inoculating directly from the tissue of an organism to culture media is
Primary cell culture
Secondary cell culture
Cell lines
Transformed cell culture
The cell culture environment
includes
media and media components
the gas composition
the plastic the cells are
attached to
all of the above
Who was awarded Nobel price for the discovery of iPSCs?
John Gurdon
Francis Collin
James Watson
Shinya Yamanaka
Primary cells have
Limited replication capacity
Unlimited replication capacity
Both of these
None of these
If the A549 (lung cancel cell line) has taken 24h to become 50% confluent from 25% confluent, how long it will take to reach full confulence?
18h
24h
36h
72h
Which of the following is NOT an advantage of animal cell culture?
Homogeneity of cells can be maintained
Cells can be preserved in liquid nitrogen for very long time.
Control of the physio-chemical environment
Cells isolated from tissues tend to go reversal in differentiation.
Primary cells stop growing after few replications, because
Cell cycle check points
Telomere shortening
Hayflick's Limit
All of the above
The growth of animal cells in vitro in a suitable culture medium is called_
Gene expression
Tranformation
Plant Cell Culture
Animal cell culture
Why is confluence important to consider when working with adherent or suspended cells?
Cells can spread out if the area is confluent.
Cells can thrive and grow well when its confluent.
Cells can adhere better to the flask when confluent.
Cells can not thrive and grow well when there's confluence.
Which of the following are non-adherent cells?
Skin cells
Muscle cells
Neurons
RBCs
