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WorksheetsSterilization
Total questions: 12
Worksheet time: 11mins
Name
Reg. No.
What do you mean by sterilization?
Formulation of media
Elimination of contamination
Recovery of products
Purification of products
3. Which of the following instrument works on the principle of batch sterilization?
LAF
Centrifuge
Autoclave
Incubator
The highest feasible temperature for batch sterilization is ________
121°C
122°C
120°C
124°C
Which one of the following is the most appropriate disinfectant for cleaning the work area (Laminar Flow Cabinet)?
saline
water
70%ethanol
90%ethanol
A student is creating fungal isolates by transferring them from an agar plate with multiple colonies to a fresh plates with single colonies. How should she dispose of the old petri dishes and her gloves that she was wearing?
Place them in a biohazard bag, autoclave them, and put the labeled bag in the general waste bin.
Check MSDS for correct disposal, put the waste in a correctly labeled container and dispose of it following SDS guidelines.
Pour any liquids down the drain and throw any trash in the garbage can.
Send the items to the school trash incinerator.
When cleaning lab spill you should NEVER
Pick up materials with hands
Wear jewelry
Start a sentence with a proposition
Breathe
What is the main danger associated with using an autoclave?
Burns associated with touching the interior and hot equipment while unloading the autoclave.
Explosion of the autoclave due to all safety valves failing and overheating of the unit.
Workers being crushed by the autoclave when it falls out of wall/off of shelves.
Workers accidentally becoming trapped inside of the autoclave and suffocating.
