WorksheetsMICRO LAB MODULE 1 PART 1 QUIZ
Total questions: 25
Worksheet time: 21mins
A used agar with pathogens should be autoclaved before disposal to a container.
TRUE
FALSE
Sterilize inoculating loop by flame sterilization until red-hot. Allow the loop to cool by blowing it dry.
TRUE
FALSE
Disinfection of the working table using 70% alcohol or Lysol will destroy endospores and kill vegetative cells.
TRUE
FALSE
The effect of ordinary refrigerator on the organisms is merely inhibition of growth.
TRUE
FALSE
An example of potential contamination of microbial cultures is:
Placing test tube caps on the tabletops
Keeping agar plates open for long periods of time
Forgetting to heat the inoculating loops
All of the above
The instrument used in the inoculation of a deep tubed media:
Inoculating loop
Inoculating needle
Sterile swab
Forceps
Arrange the following on the proper doffing of PPE:
(e.g. A,B,C,D,E)
A. Laboratory gown
B. Face mask
C. Face shield
D. Gloves
E. Shoe cover
E,D,C,A,B
A,B,D,C,E
E,D,A,C,B
A,B,C,D,E
It serves as the primary means to protect the laboratory worker and the surrounding environment from pathogens.
Biological refrigerator
PPE
HEPA Filter
Microbiological safety cabinet
Used in microbiology to detect production of gas by microorganisms.
Durham tubes
Screw capped tubes
Gas Pak Jar
UV-VIS
Sweet and salty foods frequently don't require refrigeration to prevent spoilage because:
They contain toxic alkaline chemicals
They have high solute concentration
They usually have low pH
They have insufficient nutrients to support microbial growth
A process that uses 100oC within 10 minutes. It effectively kills vegetative states like viruses and fungi.
Direct incineration
Tyndallization
Boiling
Autoclaving
The process of disinfecting places and utensils used by the public to reduce the number of pathogenic microbes to meet accepted public health standards:
Sanitation
Pasteurization
Degerming
Disinfection
Which among the following is used to prevent infection by killing or inhibiting microbial growth on inanimate objects?
Bacteriostatic agent
Sanitizer
Antiseptic
Disinfectant
It consists of an illuminated transparent plate divided into sections of known area.
Rotary shaker
Inoculating loop
Colony counter
ELISA reader
It is used to preserve cultures of bacteria, yeast, and other microbes at temperature between 2-8 °C.
Biological refrigerator
Deep freezer
Incubator
Lyophilizer
It is used to measure the growth of microbial culture in a liquid medium.
Deep freezer
Colony counter
Microtiter plates
UV-VIS spectrophotometer
Any procedure that is performed under sterile conditions and will prevent the entry of microorganisms
Antiseptic
Aseptic technique
Disinfection
Microbial growth
Infectious wastes should be disposed in the specific waste bag:
Yellow bag
Red bag
White bag
Black bag
What is the function of the following apparatus?
It is used for sterilizing culture media.
It is used in the cultivation of mammalian cells.
It serves as the primary means to protect the laboratory worker and the surrounding environment from pathogens.
It is used for the preservation of microorganism.
Broth liquid media contains 5% of Agar.
TRUE
FALSE
The main difference between nutrient agar and nutrient broth is that nutrient agar is a liquid medium whereas nutrient broth is a solid medium.
TRUE
FALSE
A method of desiccation where the sample is subjected to rapidly freezing environment under vacuum so that water is removed by sublimation.
Osmotic pressure
Lyophilization
Tyndaliization
Inspissation
The pore size of the filter must be 0.22-0.45 um that effectively separate bacteria while pore size around 20 nm is used for viruses.
TRUE
FALSE
This apparatus is a heat treatment for milk, ice-cream or even fruit juices and dairies that kills all pathogens and most non pathogens.
Autoclaving
Pasteurization
Incineration
Boiling
Have a break, have a BONUS POINT!!
Tell me, how have you been doing lately? Are you catching up on your studies? Is microbiology treating you well? 😉
