WorksheetsBacte Assess
Total questions: 50
Worksheet time: 25mins
A test for the hydrolysis of esculin in the presence of bile is especially useful in identifying species of the genus:
Corynebacterium
Listeria
Enterococcus
Staphylococcus
A patient with a history of antibiotic use presents with diarrhea. Upon endoscopy, a pseudomembrane or plaque was observed. The most likely associated organism is:
Corynebacterium diphtheriae
Arcanobacterium haemolyticum
Streptococcus pyogenes
Clostridium difficile
Which of the following is bound on the cytoplasmic membrane of Staphylococcus aureus which has a high affinity for the Fc receptor on IgG molecules as well as complement?
M protein
Protein A
PVL
Hyaluronidase
Abiotrophia spp. (formally known as nutritionally variant streptococci) will not grow on blood or chocolate agars unless supplied with:
Pyridoxal
Hemin
Vitamin B12
Cysteine
Which of the following statements is incorrect?
S. pyogenes is the only species of beta-hemolytic streptococci that will give a positive PYR reaction
S. agalactiae is able to hydrolyze hippurate and is positive in the CAMP test.
S. pneumoniae is resistant to optochin.
None of the above
A gram-positive coccus that is catalase positive, lysostaphin susceptible, and modified oxidase negative is best identified as a member of the genus
Micrococcus
Staphylococcus
Streptococcus
Pediococcus
An infant was brought to the emergency room appearing limp and “floppy”. The mother stated that she has just started to introduce table foods to her baby’s diet. A few days prior, she noted her baby to be constipated and to have diminished suckling and crying. This condition was most likely caused by:
Spoiled milk consumption
Respiratory droplets
Consumption of raw honey
All of the above
The etiologic agent most commonly associated with septicemia and meningitis of newborns is:
Streptococcus agalactiae
Streptococcus pyogenes
Streptococcus pneumoniae
Streptococcus bovis group
The etiologic agent of erysipelas is:
Streptococcus pneumoniae
Erysipelothrix rhusiopathiae
Streptococcus pyogenes
Listeria monocytogenes
The production of H2S is one characteristic used to differentiate which of the aerobic gram-positive bacilli?
Corynebacterium
Lactobacillus
Nocardia
Erysipelothrix
Which one of the following is not appropriate when describing Mycoplasma hominis?
Urease negative
Produces large colonies with a characteristic fried egg appearance
Stained with Dienes
Produces hemolytic, pinpoint colonies that stains with Gram Stain
What compound is only found in Gram-positive bacteria?
Peptidoglycan
Teichoic acid
Capsule
Outer Membrane
Which of the following stains the metachromatic granules of Corynebacterium diphtheriae?
Leifson
Ziehl-Neelsen
Methylene blue
Calcofluor white stain
Which Shigella species ferments mannitol and is ONPG and orntihine decarboxylase positive?
Shigella dysenteriae
Shigella flexneri
Shigella sonnei
Shigella boydii
Which of the following statements regarding Yersinia enterocolitica is incorrect?
Y. enterocolitica grows better with cold enrichment.
Motility is clearly noted at 25° C but not at 35° C.
CIN agar is a selective medium used to detect the presence of Y. enterocolitica.
The organism cannot be isolated on routine isolation media.
Yersinia pestis and Yersinia enterocolitica are both:
Indole negative
Methyl red positive
Phenylalanine deaminase negative
All of the Above
Which of the following differentiates Proteus vulgaris from Proteus mirabilis?
I. Indole
II. H2S on TSI
III. Phenylalanine deaminase test
IV. Methyl Red
I, II, IV
I, IV
All of the Above
I only
A positive Simmons citrate test is seen as a
Blue color
Green color
Yellow color
Red color
Steam autoclave's setting includes:
121°C, 15 psi, 15 minutes
101°C, 25 psi, 15 minutes
125°C, 15 psi, 25 minutes
112°C, 10 psi, 10 minutes
Which of the following is not a characteristic of this bacterium which is associated mainly with UTIs predominantly in female adolescents and young women?
Gram-positive cocci
Coagulase positive
Novobiovin resistant
None of the Above
Alpha-hemolysis is seen on blood agar plate as
No change in agar
Clear area around colony
Greenish discoloration of area around colony
Small area of intact red blood cells around colony surrounded by a wider zone of complete hemolysis
An organism produces a double hemolysis on egg yolk agar. This is due to what toxin
Alpha toxin
Beta toxin
Hyaluronidase
Exotoxin A
Obligate intracellular organisms:
I. Chlamydia
II. Mycoplasma
III. Rickettsia
IV. Viruses
I, II, III, IV
IV only
All except II
II, IV
A patient presents with hematuria and hypertension as a case of PSGN. Which of the following tests is used to document prior impetigo?
CBC
ASO Titer
Anti-DNAse B
Elek Test
Which of the manifestations is part of the clinical picture of the tertiary stage of syphilis?
Soft chancre
Gummas
Condyloma lata
Hard chancre
All of the following bacteria have capsules EXCEPT:
Haemophilus influenzae
Streptococcus pneumoniae
Klebsiella pneumoniae
Pseudomonas aerigunosa
All are encapsulated
Thayer-Martin agar contains the following antibiotics, EXCEPT:
Nystatin
Trimethoprim
Vancomycin
Colistin
Which of the following is used as a prophylactic treatment to prevent gonococcal (Neisseria gonorrhoeae) conjunctivitis in newborns?
Erythromycin
Bacitracin
Silver nitrate drops
All of the Above
BCYE medium is used to isolate
Corynebacterium diphtheria
Bordetella pertussis
Legionella pneumophila
Listeria monocytogenes
On what phase of the bacterial growth curve does beta-Lactam antibiotics act on?
Lag phase
Log phase
Stationary phase
Death phase
Psittacosis is a lower respiratory infection in humans caused by contact with what animal?
Rodents
Dogs
Birds
Cats
Class III biologic safety hood provides the highest level of personal protection. It is the most common in microbiology laboratories.
First statement is correct, Second statement is incorrect
First statement is incorrect, Second statement is correct
Both statements are correct
Both statements are incorrect
Which biosafety cabinet is being described? 30% Recirculated through HEPA, 70% exhausted
II, A1
II, B1
I, B2
II, A2
Which of the following species of Bacillus is nonmotile?
B. cereus
B. anthracis
Which of the following correctly describes Escherichia coli O157:H7 on MacConkey-sorbitol agar?
Lactose fermenter
Sorbitol fermenter
Lactose nonfermenter
Sorbitol nonfermenter
Ethylhydrocupreine HCl susceptibility is a presumptive test for the identification of:
Viridans streptococci
Streptococcus pyogenes
Streptococcus agalactiae
Streptococcus pneumoniae
A beta-hemolytic, catalase and esculin hydrolysis-positive, gram-positive bacteria was isolated. Growth was also observed in 6.5% NaCl. This is most likely:
Listeria monocytogenes
Corynebacterium diphtheriae
Streptococcus agalactiaeStreptococcus agalactiae
Enterococcus faecalis
Fecal cultures are inoculated on thiosulfate-citrate-bile salts-sucrose agar specifically for the isolation of:
Shigella
Salmonella
Vibrio
Campylobacter
Neisseria lactamica closely resembles Neisseria meningitidis but can be differentiated from it by its ability to metabolize:
Maltose
Glucose
Lactose
Sucros
An oxidase-negative, gram-negative bacillus that produces an alkaline slant and acid butt on triple sugar iron (TSI) agar is able to ferment which of the following carbohydrates?
Glucose only
Glucose and lactose and/or sucrose
Lactose only
Lactose and sucrose, but not glucose
Which of the following nonphotochromogen slow grower Mycobacteria species will be ruled out if niacin is negative?
Mycobacterium gastri
Mycobacterium tuberculosis
Mycobacterium avium-intracellulare
Mycobacterium xenopi
All of the following are rapidly growing mycobacteria, EXCEPT:
M. fortuitum
M. chelonae
M. smegmatis
M. scrofulaceum
Which of the following antibiotics is affected, mostly seen with P. aeruginosa, by concentration of cations in the environment?
Sulfonamide
Tetracycline
Aminoglycosides
Erythromycin
Which of the following test results is most helpful in categorizing an isolate as a member of the Tribe Proteae?
MR (-), VP (+)
Urease positive
Able to ferment lactose
PAD (+)
Which of the following exhibits a gliding motility?
Campylobacter
Capnocytophaga
Listeria
Proteus
What is the appearance of the colonies of Shigella spp. in XLD agar?
colorless colonies
yellow colonies
red colonies with black centers
colorless colonies with black centers
An organism that can live and grow in reduced concentrations of oxygen but prefers an anaerobic environment is known as a/an:
Capnophile
Obligate anaerobe
Facultative anaerobe
Aerotolerant anaerobe
Which of the following produces a toxin similar to Shiga toxin produced by Shigella dysenteriae
EIEC
EHEC
ETEC
EAEC
Which of the following correctly describes the violet pigment, violacein produced by Chromobacterium violaceum?
I. Ethanol-soluble
II. Ethanol-insoluble
III. Water-soluble
IV. Water-insoluble
I, III
II, IV
I, IV
II, III
What step in the Gram stain distinguishes between gram-positive and gram-negative organisms?
Fixing of the cells to the slide using heat
Decolorization using acetone alcohol
Counterstaining using Safranin
Application of Gram's Iodine
