WorksheetsHISTOLOGY TECHNIQUE
Total questions: 46
Worksheet time: 26mins
Which of these are Histology Technique procedures?
grossing and fixation
tissue processing and embedding
sectioning and staining
slide viewing
What does 'Grossing' means in the Grossing step of Histology Technique procedure?
to cut the tissue into a thin section
to observe the tissue and describe
to observe the tissue microscopically
Who is responsible to do the Grossing step?
Science officer
MLT
Pathologist
Microbiologist
What should we do after finish observing the specimen?
Cut the wanted site and place in plastic cassette
Do tissue processing
Cut into thin pieces using microtome
Process the tissue into a paraffin wax
Which of these are the CORRECT components of grossing a specimen?
Rapid transfer of specimen from surgery to lab
Ignoring special studies requested on the Request Form
Recording the site from which block of tissues are taken
Description of specimen's normal and abnormal features
Choose the CORRECT characteristics of a good grossing station.
Hands-free operation
High efficiency exhaust system
Stainless steel construction
Static hand held spray
A standard cassette should be used after grossing the specimen. Which of these are NOT the factors to be considered when selecting cassettes?
Has a tight fitting lids
Resistant to organic solvent
Perforated (berlubang-lubang)
The color of the cassette
After grossing, what is the next step of the Histology Technique?
Fixation
Tissue processing
Embedding
Trimming
Which of these are NOT the aim of fixation in Histology Technique
To prevent autolysis and bacterial decomposition
To fix tissue so that it won't change shape or volume
To leave the tissue as close to their living state
To enhance the tissue's component by giving them colors.
Which of these are NOT fixatives used in the fixation step of Histology Technique?
Aldehydes
Mercurial
Alcohols
Oxidizing agents
Strong acid
Formaldehyde (formalin) is a type of fixative used during the fixation step of the Histology Technique. What is the advantage of formaldehyde?
Toxic and carcinogenic
Suitable for routine staining (H&E)
Forms black pigment if leaves on standing
What is the advantage of using Mercurial as fixative in the fixation step of Histology Technique
Penetrate poorly
Cause tissue hardness
Fast and give excellent nuclear detail
Must be disposed carefully
Why is Alcohols (methanol or ethanol) are not routinely used as a fixative?
They cause brittleness and hardness
They are fast
They give a good nuclear detail
They are protein denaturants
Which of these are the factors affecting fixation step in Histology Technique?
Penetration
Volume
Temperature
Concentration
Cost
Why do we use Formalin as a routine fixative?
Can be used with H&E staining
The most forgiving
No tissues it will harm significantly
Can affect stainability of tissue when left too long
These are the steps in Tissue processing, EXCEPT
Dehydration
Clearing
Infiltration
Sectioning
Which of these is INCORRECT?
DEHYDRATION - Alcohol
CLEARING - Xylene
INFILTRATION - Methanol
These are Clearing agents of the Tissue processing step in Histology Technique, EXCEPT
Xylene
Toluene
Acetone
Ethanol
What is the function of dehydration step of Tissue processing in Histology Technique?
To remove water from tissue
To add water into the tissue
To exchange alcohol in the tissue
To add alcohol in the tissue
These are the disadvantages of Manual method of Tissue processing in the Histology Technique, EXCEPT
Labour intensive
Health hazardous
Prone to error
Self calculated time
These are the aim of Tissue processing, EXCEPT
To give the tissue a sufficient rigidity
To enable thin sections to be cut
Soft enough not to damage microtome's knife
To allow staining of the tissue
Why do we use an increasing percentage of alcohol during Tissue processing? (70%-100%)
To minimize tissue distortion
To maximize tissue distortion
To let water out from tissue
To let water enter the tissue
These are the things to consider when choosing a Clearing agent for the Tissue processing step in Histology Technique, EXCEPT
Type of tissue to be processed
Type of processor to be used
Cost and convenience
Volume of the clearing agent
These are the process of tissue embedding, EXCEPT
Transferring the processed tissue to a mould filled with molten wax
Cool and solidify the tissue
Slightly remove the excess paraffin before sectioning
Stain the tissue
These are the properties of paraffin wax that improved for Histology Technique, EXCEPT
Improve ribboning
Increase hardness
Decrease melting points
Decrease adhesion of specimen and wax
These are the precaution to be taken while embedding tissue, EXCEPT
Excess molten wax on the mould
No dust particles
Wax clear of clearing agent
Immediately cooled after embed the tissue with molten wax
Choose the mould systems used in embedding process of Histology Technique.
Traditional
Metal containers
Kite shape
Cassette-bases
The usage of alcohol in the Tissue processing step is for?
Rehydration
Clearing
Dehydration
Infiltration
The usage of xylene in the Tissue processing can be changed with?
Methanol
Acetone
Paraffin
Distilled water
The sectioning step can be done after ?
Tissue processing
Embedding
Grossing
Staining
Which of these is NOT the type of microtome?
Rotary microtome
Diamond microtome
Freezing microtome
Ultramicrotome
What microtome knife to be used with Rotary microtome?
Steel knife
Glass knife
Diamond knife
Non-corrosive knife
Which of these is NOT the characteristic of Rotary microtome?
Allows 2-25 micrometer thick
The knife is fixed and static
Not ideal for cutting serial section
Most commonly used
Which of these is NOT the characteristic of Ultramicrotome?
The thickness of tissue to be cut is 60-80 nanometer
Use diamond or glass knives
Cut very thinly
Mounted on metal grid like copper, gold, nickel
Which of these is the characteristic of Freezing microtome?
Examined with fluorescence microscope
Routinely used aside from rotary microtome
Take longer time to section
Using a frozen tissue specimen
These are the procedures of sectioning, EXCEPT?
Obtaining ribbon
Trimming
Putting the ribbon in a water bath
Take the floating specimen onto slide (Fishing)
Which of these is CORRECT?
Acid dye stains the acidic component
Acid stain : Eosin
Basic dye stains the basic component
Basic dye : Xylene
These are the characteristics of staining process of the Histology technique, EXCEPT
Give color to the sectioned specimen
Bring out a particular detail on the specimen
A small number of stain available
H&E is the routine stain
The full name of H&E is ?
(a)
The only problem we can get while using Eosin during staining is?
Little affinity when used alone
overstaining
Less forgiving
Stain cytoplasm poorly
Which one is NOT the step in staining process of the Histology Technique?
Deparaffinized
Rehydration
Dehydration
Clearing
Infiltration
Why do we use a decreasing percentage of alcohol (100-70%) during staining process of the Histology Technique?
To rehydrate the tissue
To dehydrate the tissue
To remove water from the tissue
To avoid the tissue from distortion
Why do we use xylene in the beginning of the staining process of the Histology Technique?
To deparaffinized the specimen
Xylene act as a clearing agent
To avoid the specimen from harm
To increase hardness of the specimen
After staining, we do .. ?
Embedding
Slide microscopic observation
Mounting
Put on cover slip
An example of a mountant used during mounting process of the Histology Technique?
Xylene
Eosin
Ethanol
DPX
These are the reason why putting a cover slip after mounting is important, EXCEPT
To protect the sample
To provide better optical quality for viewing
To preserve the tissue
To let us easily held the slide
