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Worksheets

Staining

Total questions: 20

Worksheet time: 11mins

Name
Class
Date
1.
Which part of a bacteria cell wall "holds" the stain? 
a)
plasma membrane
b)
fimbriae
c)
peptidoglycan
d)
pilus
2.
These bacteria are
a)
gram positive
b)
gram negative
c)
nonliving
d)
viral
3.
Which is applied first in gram staining?
a)
alcohol
b)
grams iodine
c)
crystal violet
d)
safranin
4.
Which is applied last in gram staining?
a)
alcohol
b)
crystal violet
c)
safranin
d)
grams iodine
5.

Which of the following is a mordant

a)

Methylene blue

b)

Iodine

c)

Crystal Violet

d)

Safranin

6.

Acid Fast staining and gram staining are both considered to be

a)

Differential stains

b)

Selective stains

c)

Simple stains

d)

Negative stains

7.

Which Stains are used in the endospore staining process

a)

Carbolfuchsin

b)

Malachite Green

c)

Crystal Violet

d)

Safranin

8.

Heat fixing is important because it __________.

a)

kills the microorganism

b)

makes the microorganism adhere to slide

c)

permit microorganism to receive stain

d)

all of the above

9.

This bacterial prepataion technique allow you to observe microorganisms motility, using a special slide called "depression slide"

(a)  

10.

Simple staining technique using positively charged dyes/stains.

a)

simple positive

b)

simple negative

11.

Simple staining technique that leaves the cells clear and unstained.

a)

simpple positive staining

b)

simple negative staining

12.

This simple staining technique will result into all colored bacteria.

a)

simple positive staining

b)

simple negative staining

13.

Acid fact staining technique differentiates species of ____________.

a)

Escherichia

b)

Streptococcus

c)

Mycobacterium

d)

Pseudomonas

14.

Which is applied first in acid fast staining?

a)

safranin

b)

methylene blue

c)

malachite green

d)

carbolfuchsin

15.

identify this image

a)

acid fast bacteria

b)

non acid fast bacteria

16.

When doin Anthony's stain method, ____________ serves as decolorizing agent and counterstain.

a)

20% copper nitrate solution

b)

25% copper sulfate solution

c)

25% copper nitrate solution

d)

20% copper sulfate solution

17.

Which method is used in this image?

a)

Ziehl-Neelsen method

b)

Anthony’s stain method

c)

India Ink method

18.

What method is used in endospore staining?

a)

India ink method

b)

Ziehl-Neelsen method

c)

Schaeffer-Fulton`s method

d)

Anthony’s stain method

19.

What is the primary stain used in endospore staining?

(a)  

20.

Which of the following is the CORRECT order of the steps of a Gram stain?

a)

Primary stain → Decolorizing agent → Mordant → Counterstain

b)

Counterstain→ Mordant → Decolorizing agent→ Primary stain

c)

Primary stain→ Mordant→ Decolorizing agent→ Counterstain

d)

Primary stain→ Counterstain→ Decolorizing agent→ Mordant