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Metabolic Activities of Bacteria

Total questions: 15

Worksheet time: 8mins

Name
Class
Date
1.

To test carbohydrate fermentation of bacteria, one must use nutrients broth with the following, except?

a)

monosaccharide

b)

peptone

c)

urea

d)

disaccharaide

2.

Which of the following do not indicate carbohydrate fermentation?

a)

bubble at the top of durham tube

b)

yellow color of medium

c)

dark colored bacteria

d)

none of the above

3.

Which of the follwoing is not true about gelatin?

a)

first used by Robert Koch

b)

Solid at temperature above 28 degree Celsius

c)

can be broken down by gelatinase enzyme

d)

all are true

4.

What enzyme can be detected when using nutrient gelatin deeps?

(a)  

5.

What enzyme breaks down starch into monosaccharides and disaccharide?

(a)  

6.

What do this image indicate?

a)

positive in amylase enzyme

b)

positive in gelatinase enzyme

c)

positive in urease enzyme

d)

positive in caseinases enzyme

7.

What enzyme is produce by some bacteria that breaks down caseins?

(a)  

8.

Letter C indicates __________.

a)

presence of caseinases

b)

absence of caseinases

9.

Urease enzyme breaks down urea into _________.

a)

ammonia

b)

carbon dioxide

c)

protein

d)

two of the above

10.

What is the pH indicator in Simmon's citrate medium?

a)

bromothymol pink

b)

bromothymol blue

c)

bromothymol green

d)

bromothymol yellow

11.

What toxic compound is a byproduct of aerobic metabolism?

a)

supermonoxide free radicals

b)

superoxide dismutase

c)

hydrogen peroxide

d)

All of the above

12.

What does bubble indicate when hydrogen peroxide is added to the bacteria?

a)

urease

b)

gelatinase

c)

catalase

d)

tryptophanase

13.

Presence of indole is detected using BBL dry slide. Presence of indole will result _______ color to the slide

a)

yellow

b)

pink

c)

green

d)

red

14.

Tryptophanase breaks down tryptophan into the following except?

a)

urea

b)

ammonia

c)

indole

d)

pyruvate

15.

It is a metabolic process that breaks down glucose into pyruvate.

(a)