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WorksheetsLaboratory Midterm Exams
Total questions: 40
Worksheet time: 28mins
This microscope allows for binocular (two eyes) viewing of larger specimens.
(a)
The flat platform where you place your slides.
(a)
It is found above the mirror consisting of several metal blades which form a circular opening that may be enlarged or reduced to control the amount of light reaching the object.
(a)
The tube attached to the arm that supports the eyepiece.
(a)
It is found below the stage with concave and flat surfaces to gather and direct the light to illuminate the object.
(a)
The models found in most schools, use compound lenses and light to magnify objects. The lenses bend or refract the light, which makes the object beneath them appear closer.
(a)
Heavy Y-shape foot on which the microscope stands.
(a)
The microscope has 3 magnifications: (a) , Low and High.
The lens found at the top of the draw tube that you look through. They are usually 10X or 15X power.
(a)
CCJE STANDS FOR (a) .
The models found in most schools, use compound lenses and light to magnify objects. The lenses bend and refract the light, which makes the objects beneath them appear closer.
Transmission Electron Microscope
Electron Microscope
Light Microscope
Steroscope
An adjustment joint between the pillar and the arm, that lets the arm tilt at various angles.
Inclination Joint
Revolving Nosepiece
Pillar
Draw Tube
It is found immediately beneath the stage, serves further to concentrate the light rays on specimen.
Low Power Objective
Condenser
Mirror
High Power Objective
Which of the following does not show proper care for the microscope?
Secure the mounted slide on the stage with one clip, preferably the right one so that the slide can be moved about easily while focusing.
Return the microscope to its proper place with the lower objective at its lowest limit, the mirror in vertical position and the clips directed forward
Never let the lenses come in contact with any chemical.
Always carry microscope with two hands, your left supporting the base and your right grasping the handle firmly and set them flat on your desk.
It is a metallic structure above the nosepiece which protects the lower lenses.
Arm
Draw Tube
Dust Shield
Pillar
A mechanism attached to the stage that will enable to move the slide around by turning two knobs. One moves it left and right, the other moves it up and down.
Coarse Focus Adjustment
Fine Focus Adjustment
Mechanical Stage
Stage
In making a wet mount, we should:
Place the cover slip at a 35 degree angle (approximately) with one edge touching the water drop and then gently let go
Place the cover slip at a 35 degree angle (approximately) with both edge touching the water drop and then gently let go
Place the cover slip at a 45 degree angle (approximately) with one edge touching the water drop and then gently let go
Place the cover slip at a 45 degree angle (approximately) with both edge touching the water drop and then gently let go
During clean-up, the following should be done except:
Store microscopes with the scanning objective in place
Wrap cords and cover microscopes.
Wash cover slips and returned in their respective containers.
Wash slides in the sinks and dry them, placing them back in the slide boxes to be used later
Only half of my viewing field is lit, it looks like there's a half-moon in there!
Remember the steps, if you can't focus under scanning and then low power, you won't be able to focus anything under high power.
You probably don't have your objective fully clicked into place.
Adjust the diaphragm, make sure your light is on.
Your lens is dirty. Use lens paper, and only lens paper to carefully clean the objective and ocular lens. The ocular lens can be removed to clean the inside.
In the collection of Liquid Blood, the following procedure must be done except:
Collect the blood sample by picking up with a medicine dropper and place it in a test tube containing sodium fluoride.
Cover the test tube with stopper and seal.
With the use of a razor blade or clean knife, loose and scrape the dried material onto the paper. Crust should be removed without breaking if possible.
Label the test tube bearing the initials or name of the investigator and date of the collection and sealing. Label maybe in the form of paper pasted on the container or tag tied to the object.
Arrange the following procedure in the proper collection and packing of Blood on large solid surface correctly.
1. With the use of a razor blade or clean knife, loose and scrape the dried material onto the paper. Crust should be removed without breaking if possible.
2. Shake the powdered stain to the center of the paper and then fold. Scrapings removed from the different areas of the walls and floors are separately placed on a paper and labeled as to the location where found.
3. Place clean sheet of paper below the surface bearing the stain.
4. Seal thoroughly the folded paper with cellophane tape and individually place inside a white mailing envelope sealed with a masking tape and affix the initials of the investigator, date and other information.
1,2,3,4,
3,2,1,4
2,1,3,4
3,1,2,4
In the proper collection and packing of Blood Stain on Clothing and Fabrics, which of the following is not a part of the procedure?
Place identifying marks directly on the cloth as far away from stained areas as possible
Collect the blood sample by picking up with a medicine dropper and place it in a test tube containing sodium fluoride.
Place the bag containing the items in the coldest, driest facility possible until it is sent to the laboratory.
Wrap and pack separately the clothing in a close paper container such as paper bag.
A Dutch spectacle maker who is credited for the invention of the compound microscope
Zachary Janssen
Zachary Jansen
Zacharias Janssen
Zacharias Jannes
What is the purpose of adding Sodium Fluoride to the liquid blood sample?
generate most of the chemical energy needed to power the blood cells biochemical reactions.
serve as substrates upon which other substances are formed
to prevent red cell glycolysis and to determine an accurate blood glucose level.
it makes the liquid blood dry
The compound microscope has two system lenses for greater magnification, (1) the objective lens that one looks into and (2) the ocular or eyepiece lens or the lens closest to the object.
TRUE
FALSE
The Arm is an optical part of the microscope, which is short-curved, used in carrying the microscope. This attaches the eyepiece and the body tube to the base.
TRUE
FALSE
Fine Focus Adjustment is a knob geared to the body tube use to bring the object into focus.
TRUE
FALSE
High Power Magnification has a total of 400X Magnification because the objective has a magnification of 4X and the Ocular Lens has 100X.
TRUE
FALSE
Transmission Electron Microscope also uses electrons, but instead of scanning the surface, electrons are passed through very thin specimens.
TRUE
FALSE
High Power Objective is a large lens with high magnifying power. Shorter lens screwed to the rotating nosepiece; serves to form bigger image of the object within the body tube.
TRUE
FALSE
The Low Power Objective has a magnification of 10X.
TRUE
FALSE
Look through the ocular with one eye and adjust the mirror for even illumination. Never use direct sunlight when you look through the microscope as it may injure your eye. Keep both eyes open and relaxed.
TRUE
FALSE
In Focusing specimens, always start with the scanning objective. Use the Coarse Knob to focus, image may be small at this magnification, but you won't be able to find it on the higher powers without this first step. Use stage clips immediately, try moving the slide around until you find something.
TRUE
FALSE
In focusing specimens, If the specimen is too light or too dark, try adjusting the eyepiece. TRUE
TRUE
FALSE
In using the microscope, look through the ocular and slowly turn the coarse adjustment screw counter clockwise until the object is clearly in view; then use the fine adjustment to get a sharp image. Never focus with the coarse adjustment while looking through the microscope as you are liable to crush the slide and damage the lens. If you cannot see the object, it means you have not placed it at the center of the microscope field. Re-center the object and repeat the focusing procedure until you see it.
TRUE
FALSE
In using the microscope, look through the ocular and slowly turn the coarse adjustment screw counter clockwise until the object is clearly in view; then use the fine adjustment to get a sharp image. Never focus with the coarse adjustment while looking through the microscope as you are liable to crush the slide and damage the lens. If you cannot see the object, it means you have not placed it at the center of the microscope field. Re-center the object and repeat the focusing procedure until you see it.
TRUE
FALSE
Labels should be written inside the circle when drawing specimens.
TRUE
FALSE
In staining a slide, the stain does not need to be under the entire cover slip.
TRUE
FALSE
For blood stains in hard objects, place identifying marks directly on the recovered object as far away from stained areas as possible. Provide a label or a string tag bearing only the following information: Date and Time of finding the specimen, name and description of the specimen, signature of the investigator.
TRUE
FALSE
The Hydrogen Peroxide catalyzes the oxidation of phenolphthalein, which is colorless in solution, into phenolphthalein resulting in a pink colored solution.
TRUE
FALSE
