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Laboratory Midterm Exams

Total questions: 40

Worksheet time: 28mins

Name
Class
Date
1.

This microscope allows for binocular (two eyes) viewing of larger specimens.

(a)  

2.

The flat platform where you place your slides.

(a)  

3.

It is found above the mirror consisting of several metal blades which form a circular opening that may be enlarged or reduced to control the amount of light reaching the object.

(a)  

4.

The tube attached to the arm that supports the eyepiece.

(a)  

5.

It is found below the stage with concave and flat surfaces to gather and direct the light to illuminate the object.

(a)  

6.

The models found in most schools, use compound lenses and light to magnify objects. The lenses bend or refract the light, which makes the object beneath them appear closer.

(a)  

7.

Heavy Y-shape foot on which the microscope stands.

(a)  

8.

The microscope has 3 magnifications: (a)   , Low and High.

9.

The lens found at the top of the draw tube that you look through.  They are usually 10X or 15X power. 

(a)  

10.

CCJE STANDS FOR (a)   .

11.

The models found in most schools, use compound lenses and light to magnify objects. The lenses bend and refract the light, which makes the objects beneath them appear closer.

a)

Transmission Electron Microscope

b)

Electron Microscope

c)

Light Microscope

d)

Steroscope

12.

  An adjustment joint between the pillar and the arm, that lets the arm tilt at various angles.

a)

Inclination Joint

b)

Revolving Nosepiece

c)

Pillar

d)

Draw Tube

13.

It is found immediately beneath the stage, serves further to concentrate the light rays on specimen.

a)

  Low Power Objective

b)

Condenser

c)

Mirror

d)

High Power Objective

14.

Which of the following does not show proper care for the microscope?

a)

Secure the mounted slide on the stage with one clip, preferably the right one so that the slide can be moved about easily while focusing.

b)

Return the microscope to its proper place with the lower objective at its lowest limit, the mirror in vertical position and the clips directed forward

c)

Never let the lenses come in contact with any chemical.

d)

Always carry microscope with two hands, your left supporting the base and your right grasping the handle firmly and set them flat on your desk.

15.

It is a metallic structure above the nosepiece which protects the lower lenses.

a)

Arm

b)

Draw Tube

c)

Dust Shield

d)

Pillar

16.

A mechanism attached to the stage that will enable to move the slide around by turning two knobs.  One moves it left and right, the other moves it up and down. 

a)

Coarse Focus Adjustment

b)

Fine Focus Adjustment

c)

Mechanical Stage

d)

Stage

17.

  In making a wet mount, we should:

a)

Place the cover slip at a 35 degree angle (approximately) with one edge touching the water drop and then gently let go

b)

Place the cover slip at a 35 degree angle (approximately) with both edge touching the water  drop  and then gently let go

c)

Place the cover slip at a 45 degree angle (approximately) with one edge touching the water  drop  and then gently let go

d)

Place the cover slip at a 45 degree angle (approximately) with both edge touching the water  drop  and then gently let go

18.

During clean-up, the following should be done except:

a)

Store microscopes with the scanning objective in place

b)

Wrap cords and cover microscopes.

c)

  Wash cover slips and returned in their respective containers.

d)

Wash slides in the sinks and dry them, placing them back in the slide boxes to be used later

19.

Only half of my viewing field is lit, it looks like there's a half-moon in there!  

a)

Remember the steps, if you can't focus under scanning and then low power, you won't be able to focus anything under high power.

b)

  You probably don't have your objective fully clicked into place.

c)

  Adjust the diaphragm, make sure your light is on.

d)

Your lens is dirty. Use lens paper, and only lens paper to carefully clean the objective and ocular lens. The ocular lens can be removed to clean the inside.

20.

   In the collection of Liquid Blood, the following procedure must be done except:

a)

Collect the blood sample by picking up with a medicine dropper and place it in a test  tube containing sodium fluoride.

b)

Cover the test tube with stopper and seal.

c)

With the use of a razor blade or clean knife, loose and scrape the dried material onto the paper. Crust should be removed without breaking if possible.

d)

       Label the test tube bearing the initials or name of the investigator and date of the collection and sealing. Label maybe in the form of paper pasted on the container or tag tied to the object.

21.

Arrange the following procedure in the proper collection and packing of Blood on large solid surface correctly.

1.       With the use of a razor blade or clean knife, loose and scrape the dried material onto the paper. Crust should be removed without breaking if possible.

2.       Shake the powdered stain to the center of the paper and then fold. Scrapings removed from the different areas of the walls and floors are separately placed on a paper and labeled as to the location where found.

3.       Place clean sheet of paper below the surface bearing the stain.

4.       Seal thoroughly the folded paper with cellophane tape and individually place inside a white mailing envelope sealed with a masking tape and affix the initials of the investigator, date and other information.

a)

   1,2,3,4,

b)

   3,2,1,4

c)

    2,1,3,4

d)

3,1,2,4

22.

In the proper collection and packing of Blood Stain on Clothing and Fabrics, which of the following is not a part of the procedure?

a)

  Place identifying marks directly on the cloth as far away from stained areas as possible

b)

Collect the blood sample by picking up with a medicine dropper and place it in a test  tube containing sodium fluoride.

c)

Place the bag containing the items in the coldest, driest facility possible until it is sent to the laboratory.

d)

Wrap and pack separately the clothing in a close paper container such as paper bag.

23.

A Dutch spectacle maker who is credited for the invention of the compound microscope

a)

Zachary Janssen

b)

Zachary Jansen

c)

  Zacharias Janssen

d)

Zacharias Jannes

24.

What is the purpose of adding Sodium Fluoride to the liquid blood sample?

a)

generate most of the chemical energy needed to power the blood cells biochemical reactions.

b)

serve as substrates upon which other substances are formed

c)

  to prevent red cell glycolysis and to determine an accurate blood glucose level.

d)

it makes the liquid blood dry

25.

The compound microscope has two system lenses for greater magnification, (1) the objective lens that one looks into and (2) the ocular or eyepiece lens or the lens closest to the object.

a)

TRUE

b)

FALSE

26.

The Arm is an optical part of the microscope, which is short-curved, used in carrying the microscope. This attaches the eyepiece and the body tube to the base.

a)

TRUE

b)

FALSE

27.

  Fine Focus Adjustment is a knob geared to the body tube use to bring the object into focus.

a)

TRUE

b)

FALSE

28.

  High Power Magnification has a total of 400X Magnification because the objective has a magnification of 4X and the Ocular Lens has 100X.

a)

TRUE

b)

FALSE

29.

Transmission Electron Microscope also uses electrons, but instead of scanning the surface, electrons are passed through very thin specimens.

a)

TRUE

b)

FALSE

30.

High Power Objective is a large lens with high magnifying power. Shorter lens screwed to the rotating nosepiece; serves to form bigger image of the object within the body tube.

a)

TRUE

b)

FALSE

31.

The Low Power Objective has a magnification of 10X.

a)

TRUE

b)

FALSE

32.

Look through the ocular with one eye and adjust the mirror for even illumination. Never use direct sunlight when you look through the microscope as it may injure your eye. Keep both eyes open and relaxed.

a)

TRUE

b)

FALSE

33.

In Focusing specimens, always start with the scanning objective. Use the Coarse Knob to focus, image may be small at  this  magnification, but  you won't be able to find it on the higher powers without this  first step. Use stage  clips immediately, try moving the slide around until you find something.

a)

TRUE

b)

FALSE

34.

  In focusing specimens, If the specimen is too light or too dark, try adjusting the eyepiece. TRUE

a)

TRUE

b)

FALSE

35.

In using the microscope, look through the ocular and slowly turn the coarse adjustment screw counter clockwise until the object is clearly in view; then use the fine adjustment to get a sharp image. Never focus with the coarse adjustment while looking through the microscope as you are liable to crush the slide and damage the lens. If you cannot see the object, it means you have not placed it at the center of the microscope field. Re-center the object and repeat the focusing procedure until you see it.

a)

TRUE

b)

FALSE

36.

In using the microscope, look through the ocular and slowly turn the coarse adjustment screw counter clockwise until the object is clearly in view; then use the fine adjustment to get a sharp image. Never focus with the coarse adjustment while looking through the microscope as you are liable to crush the slide and damage the lens. If you cannot see the object, it means you have not placed it at the center of the microscope field. Re-center the object and repeat the focusing procedure until you see it.

a)

TRUE

b)

FALSE

37.

Labels should be written inside the circle when drawing specimens.

a)

TRUE

b)

FALSE

38.

In staining a slide, the stain does not need to be under the entire cover slip.

a)

TRUE

b)

FALSE

39.

For blood stains in hard objects, place identifying marks directly on the recovered object as far away from stained areas as possible. Provide a label or a string tag bearing only the following information: Date and Time of finding the specimen, name and description of the specimen, signature of the investigator.

a)

TRUE

b)

FALSE

40.

The Hydrogen Peroxide catalyzes the oxidation of phenolphthalein, which is colorless in solution, into phenolphthalein resulting in a pink colored solution.

a)

TRUE

b)

FALSE