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Worksheets

Microbiology

Total questions: 33

Worksheet time: 25mins

Name
Class
Date
1.

Extremophiles which can survive under highly acidic environment are called as ___________

a)

Barophiles

b)

Acidophiles

c)

Alkalophiles

d)

Thermophilea

2.

Which of the following is a correct source where acidophiles are located

a)

Freshwater

b)

Saltwater lakes

c)

Acid mine drainage

d)

Acid rain

3.

Extremophilic acidophiles are found at pH __

a)

pH 2.2

b)

pH 7

c)

pH 6.5

d)

pH 9

4.

Acidophilic bacteria involved in making acetic acid ____

a)

Thiooxidans ferroxidans

b)

Acidophilus thiooxidans

c)

Acetobacter aceti

d)

Thermopasmatales

5.

Acidophile involved in bioleaching of metal is

a)

Acetobacter aceti

b)

Lactobacillus acidophillus

c)

Leuconostoc spp.

d)

Thiobacillus ferroxidans

6.

Which of the following mechanism is used by acidophilies to maintain inner pH?

a)

Apoptosis

b)

Cytolysis

c)

Proton efflux

d)

Phagocytosis

7.

Acidophiles belong to which group of following

a)

Archeae

b)

Eubacteria

c)

Both archeae and eubacteria

d)

None of the above

8.

Acidophile can synthesis pH resisting enzyme like _____

a)

Pepsin

b)

Lipase

c)

Kinase

d)

Trypsin

9.

Impermeability of cell membrane of an acidophile is due to presence of

a)

Tetra ether lipids

b)

Muramic acid

c)

Phospholipids

d)

Sphingo lipids

10.

You can find extremophiles in

a)

Laboratory

b)

Hydrosphere

c)

Volcanic springs

d)

Freshwater

11.

Active mechanism used by acidophiles to survive highly acidic environment

a)

Proton efflux pump

b)

DNA repair system

c)

Chemotaxis

d)

Quoram sensing

12.

Which of the following has acidic cytoplasm

a)

Acetobacter aceti

b)

E.coli

c)

Rhizophus

d)

Bacillus thurengenesis

13.

Acidophile found in volcanic springs having optimum growth pH 2 to 3 is

a)

Sulfolobus spp.

b)

Acetobacter aceti

c)

Bacillus spp

d)

E.coli

14.

Reduced pore size of membrane as an adaptation in _____________ can be seen which helps to survive in acidic conditions

a)

Thermoplasmas

b)

Acidthiobacillus ferooxidans

c)

Bacillus spp

d)

Acetobacter aceti

15.

Genome of ________ is smaller than neutrophiles

a)

Barophiles

b)

Psycrophiles

c)

Acidophiles

d)

Thermophiles

16.

Which of the following is an extremozyme produced by acidophiles

a)

Lipase

b)

Sulfate oxygenase

c)

Protease

d)

Amylase

17.

Enzyme providing adaptability to extremophiles is called

a)

Isozyme

b)

Robozyme

c)

Abyzyme

d)

Extremozyme

18.

Obligate acidophiles require pH in range of

a)

pH 1-4

b)

pH 4-6

c)

pH 6-10

d)

pH 3-6

19.

Which protein in acidophiles helps in acid stability

a)

Rusticyanin

b)

Phycocyanin

c)

Pepsin

d)

None of the following

20.

Which is the best place to find acidophiles

a)

Drainage

b)

Arctic ocean

c)

Salt lake

d)

Hydrothermal vents

21.

Despite environment being acidic acidophiles manages to keep their internal pH near to neutral because of

a)

DNA repair system

b)

Chemotaxis

c)

Impermeability of cell wall

d)

Phagocytosis

22.

________ has pH below 3

a)

Arctic sea

b)

Acid mining drainage

c)

Sewage

d)

Salt lake

23.

California mine has massive growth of an acidophile called as

a)

Thermus acquaticus

b)

Ferroplasma

c)

Spirulina

d)

Leuconostoc

24.

Thiobacillus ferroxidans is involved in bioleaching of

a)

Copper

b)

Steel

c)

Charcoal

d)

Sulphur

25.

Which of the following is an example of extremophiles

a)

Acidophiles

b)

Barophiles

c)

Thermophiles

d)

All of the above

26.

Which of the following acidophile is responsible for mineralization process?

a)

Acidthiobacillus caldus

b)

Acetobacter aceti

c)

Alicyclobacillus acidoterrestris

d)

Sulfolobus spp

27.

Food spoiling acidophile

a)

Alicyclobacillus acidoterrestris

b)

Acidthiobacillus caldus

c)

Thiobacillus ferroxidans

d)

Thermoplasm acidophilum

28.

Acidthiobacillus ferooxidans cell membrane consists ___________ fatty acids

a)

Cyclopropane

b)

Beta hydroxy

c)

Omega cyclohexyl

d)

None of the above

29.

Acidophiles generates an inside positive transmembrane electric potential to resist the inward flow of protons like

a)

Na+

b)

K+

c)

Cl-

d)

None of these above

30.

Ubiquitous phenomenon that establishes cell to cell communication in bacterial population through production, secretion and detection of signal molecules is called as

a)

Chemotaxis

b)

Quorum Sensing

c)

Phagocytosis

d)

Biofilm

31.

Flagella mediated movement of bacterial cells in response to environmental stimulus like pH, temperature,osmotic pressure is called as ______

a)

Quorum sensing

b)

Chemotaxis

c)

Phagocytosis

d)

None of the above

32.

fur gene is significantly upregulated in some genes related chemotaxis in which acidophile ?

a)

Acidthiobacillus caldus

b)

Ferroplasma spp

c)

Acetobacter aceti

d)

Thiobacillus ferroxidans

33.

Glycosyl hydrolases (an extremozyme) used in antibiotic production is obtained from ___________

a)

Hydrogenomonas

b)

Acidthiobacillus caldus

c)

Acidthiobacillus ferooxidans

d)

Sulfolobus spp