Chemicals that kill all bacteria and bacterial endospores

Chemicals that kill or inhibit the growth of microbes and are designed to be used on inanimate objects

Solutions that kill viral, bacterial, and protozoan pathogens

Chemicals that generally inhibit the growth of microbes and are designed for use on skin or other tissue

The Kirby-Bauer method measures whether specific antibiotics can kill or inhibit the growth of individual bacterial strains

Microbiologists compare the color of a bacterial suspension to the McFarland No. 0.5 standard, ignoring turbidity

The Mueller Hinton antibiotic sensitivity test, accepted worldwide as a clinical standard, utilizes Kirby-Bauer medium

Filter paper disks impregnated with specific concentrations of antibiotics are commercially available

Colonies growing within the zone of inhibition around an antibiotic disk always indicate contamination

Which pair doesn't match up?

Must have oxygen to live

Utilize aerobic respiration but require increased CO2 and decreased O2 concentrations compared with those usually found in air

Do not utilize oxygen and are not killed by it

Utilize aerobic and anaerobic respiration and often also produce enzymes that carry out fermentation

Do not produce the enzyme catalase nor do they manufacture superoxide dismutase

Manufacture superoxide dismutase

Pasteur discovered anaerobes, microorganisms that are poisoned by oxygen

Fermentation releases some but not all of the energy in organic compounds

Anaerobes grow in reduced environments

Fluid Thioglycollate Medium is pink in the bottom of the tube where the resazurin is highly reduced

In the soupy, backyard hot tub of a decadent microbio prof

Cats ear

Rotting a forgotten, refrigerated cucumber

Santa's sleigh runners

The human stomach, with its pH around 2, is especially well suited for the growth of alkalophiles

Vinegar with a pH of about 3 is 100 times more acid than cheese with a pH of about 5

Most bacteria are neutralophiles

Most fungi are alkalophiles, but a few are acidophiles

Hydrogen Peroxide

Glycerol and fatty acids

Fats, oils, phospholipids, and other molecules soluble in nonpolar solvents

Starch

Hydrogen sulfide gas

Exoenzymes speed reactions outside of cells

Any bacteria that grow on a plate of starch agar are amylase producers

An oxidase is an enzyme that oxidizes compounds

Cysteine desulfhydrase yields hydrogen sulfide, hydrochloric acid, and pyruvic acid

NO3- to NO2-

Removes electrons from NO2-

NO2- to NO3-

CO(NH2)2 + H2O to 2NH3 + CO2

Involved in denitrification

Urea is a protein

Facultatively anaerobic bacteria generally do not produce nitrate reductase

If nitrite is missing from an incubated culture tube of Nitrate Broth, it may indicate that the bacteria did not produce nitrate reductase

The acid color of a Phenol Red Carbohydrate Broth is red, the alkali color is yellow

Tryptophanase hydrolyzes tryptophan into pyruvic acid and citric acid

Another name for dextrose is glucose

Columbia CNA Agar

Blood Agar

Levine EMB Agar

Pseudomonas Agar F

Tryptic Soy Agar

There is no universal medium

An isolated colony on a selective medium is not considered a pure culture

Ultraviolet radiation excites electrons in fluorescent pigment

Spirochetes and gram-negative rods with pointed ends abound on the gums of patients who suffer from Vincent's angina