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Microbial biotransformation homework 2

Total questions: 10

Worksheet time: 29mins

Name
Class
Date
1.

Contaminated soil sample contains Cr(VI) metal pollutant which is carcinogenic in nature. It can be removed by reductive absorption with six indigenous microorganism by converting Cr(VI) to Cr(III). Kelbesella pneumonial, Bacillus firm, and mycobacterium S.P are capable to absorb Cr(VI) where as fungal isolates Aspergillus S P, Aspergillus flavus and A. niger are competent in transforming Cr(VI) to Cr(III). Bacillus firm kelbesella pneumonial can tolerate 550 mg/L of Cr(VI).

This is:

a)

cell metabolic dependent activity

b)

independent of cell metabolic activity

c)

chemical bioremediation

d)

electrical bioremediation

2.

Contaminated soil sample contains Cr(VI) metal pollutant which is carcinogenic in nature. It can be removed by reductive absorption with six indigenous microorganism by converting Cr(VI) to Cr(III). Kelbesella pneumonial, Bacillus firm, and mycobacterium S.P are capable to absorb Cr(VI) where as fungal isolates Aspergillus S P, Aspergillus flavus and A. niger are competent in transforming Cr(VI) to Cr(III). Bacillus firm kelbesella pneumonial can tolerate 550 mg/L of Cr(VI).

This is microbial biotransformation.

a)

true because it performs reduction

b)

false because this is a reductive process

3.

EPS is a form of adaptation by microbe via the mechanism of bioaccumulation of heavy metal

a)

True

b)

False

4.

Bioaccumulation is a passive uptake mechanism

a)

True

b)

False

5.

Base on this picture explain bioaccumulation

4 lines
6.

Metallothioneins (MTs) are a family of small, highly conserved, cysteine-rich metal-binding proteins that are important for zinc and copper homeostasis, protection against oxidative stress, and buffering against toxic heavy metals.

This is presence in microorganism that are able to tolerate high concentration of heavy metals

a)

False

b)

True

7.

Active mechanism for HM adaptation includes the following EXCEPT:

a)

Rhamnolipid production

b)

Intracellular accumulation

c)

Oxidation/Reduction

d)

Ion Exchange reaction at the cell wall

8.

These HM adaptation mechanism is a slow active uptake , EXCEPT:

a)

Efflux pump

b)

bioaccumulation

c)

physical biosorption of cell wall

d)

oxidation/reduction

9.

Biosorption and Bioaccumulation is the same

a)

True

b)

False

10.

Biotransformation of heavy metals by microorganism is

a)

physical adsorption of the cell wall

b)

reduction of Cr(v) to Cr (III)

c)

forming complexation