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H Disease & DNA Replication

Total questions: 70

Worksheet time: 2hrs 1mins

Name
Class
Date
1.

Drag the correct labels to each section of the diagram for a nucleotide of DNA.

2.
Which of the following units are repeatedly joined together to form a strand of DNA? (This is the MONOMER of DNA.) 
a)
amino acids
b)
nucleotides
c)
fatty acids
d)
polysaccharides
3.
Why is DNA important?
a)
it is very small and very complicated
b)
it's in everything
c)
it serves as the blueprint for traits of all living things
d)
because we eat it every day for energy
4.

What are the 4 nitrogenous bases in DNA?

a)
adenine, thymine, cytoplasm, and guanine
b)
adenine, thymine cytosine, and guanine
c)

adenine, thymine, cytosine, and uracil

d)

adenine, uracil, cytosine, and guanine

5.

(a)   forms the sides (backbone) of the DNA ladder.

Choose from the below words
deoxyribose and adenine
deoxyribose and a hydrogen bond
sugar and the nucleus
deoxyribose (sugar) and phosphate
6.

DNA is usually found in the (a)   (organelle) of a cell.

7.

In a molecule of double-stranded DNA, the amount of Adenine present is always equal to the amount of (a)   .

Choose from the below words
cytosine
guanine
thymine
uracil
8.

Nitrogenous bases are joined together by what type of bond?

(a)  

9.

Replicate the following strand of DNA: 
AATCATGGA

(a)  

10.

The enzyme that unzips the DNA to prepare for replication is (a)   .

Choose from the below words
helicase
replicase
polymerase
synthase
11.
DNA replication results in two DNA molecules,
a)
each one with two original strands
b)
each one with two new strands
c)
each one with one new strand and one original strand
d)
one with two new strands and the other with two original strands
12.

The order of replication is (a)   .

Choose from the below words
3, 2, 1, 4
2, 1, 4, 3
2, 3, 1, 4
3, 4, 1, 2
13.
Which enzyme is responsible for adding nucleotides?
a)
Topoisomerase
b)
DNA Polymerase
c)
Ligase
d)
Primase
14.

The enzyme that joins the Okazaki fragments in the lagging strand is (a)   .

15.

This picture is illustrating the (a)   nature of DNA.

Choose from the below words
antiparallel
semiconservative
helical
radioactive
16.

The strand of DNA that is replicated continuously from 5' to 3' is known as (a)  

Choose from the below words
leading strand
lagging strand
parallel strand
antiparallel strand
17.

If the percentage of DNA is made of 30% Thymine, the correct percentage of Guanine is (a)   .

18.

The enzyme responsible for adding the RNA primer to the template strand is (a)   .

19.

Chromosomes are composed of (a)   .

Choose from the below words
protein only
DNA only
DNA and protein
RNA and protein
20.

What enzyme keeps DNA from overwinding ahead of replication forks?

a)

Primase

b)

Topoisomerase

c)

Single-Stand Binding Protein

d)

Helicase

21.

The primer is composed of _____.

a)

amino acids

b)

RNA only

c)

DNA only

d)

RNA and DNA

22.

How do the leading and the lagging strands differ?

a)

The leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction.

b)

The leading strand is synthesized at twice the rate of the lagging strand.

c)

The leading strand is synthesized in short fragments that are ultimately stitched together, whereas the lagging strand is synthesized continuously.

d)

The leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5? end.

23.
Which enzyme is needed to start DNA replication?
a)
DNA Polymerase
b)
Topoisomerase
c)
Ligase
d)
Primase
24.
Which of the following help(s) to hold the DNA strands apart while they are being replicated?
a)
RNA primase
b)
single–strand binding proteins
c)
DNA polymerase I
d)
DNA ligase
25.
What is meant by the description "antiparallel" regarding the strands that make up DNA?
a)
The twisting nature of DNA creates nonparallel strands.
b)
One strand is positively charged and the other is negatively charged.
c)
The 5' to 3' direction of one strand runs counter to the 5' to 3' direction of the other strand.
d)
Base pairings create unequal spacing between the two DNA strands
26.

If a portion of DNA strand reads:

3' A A A T T T C 5'

The matching strand would read (a)   .

Choose from the below words
5' T T T A A A G 3'
3' T T T A A A G 5'
5' A A A T T T C 3'
3' U U U A A A G 5'
27.

(a)   is not an enzyme used in replication.

Choose from the below words
DNA polymerase
primase
helicase
triptase
28.
A new strand of DNA is created in the ____ direction during DNA replication.
a)
3' to 5'
b)
5' to 3'
29.

The (a)   strand is made in short fragments during DNA replication

30.
Which number best represents helicase
31.
Which number best represents DNA polymerase?
32.
Which number best represents topoisomerase?
33.

Which of the following help(s) to hold the DNA strands apart while they are being replicated?

a)

single-strand DNA binding proteins

b)

Topoisomerase

c)

primase

d)

DNA ligase

34.

The correct match for polarity, the ending molecule, and the strand name is (a)  

Choose from the below words
positive; sugar; 3'
positive; sugar; 5'
phosphate; negative; 3'
phosphate; positive; 5'
35.

In which direction do enzymes (a)   ?

Choose from the below words
3'-5'
5'-3'
3'-3'
5'-5'
36.

In which direction to enzymes build new strands?

a)

5'-3'

b)

3'-5'

c)

3'-3'

d)

5'-5'

37.

The disc shaped protein core that DNA wraps around is called (a)  

Choose from the below words
histone
nucleosome
chromatin
gene
38.
direction new nucleotides are synthesized on the new strand
a)
5' to 3' (new nucleotides added to the 3' end)
b)
3' to 5' (new nucleotides added to the 5' end)
39.
Which shows the correct base-pairing rules?
a)
A-C, G-T
b)
A-T, G-C
c)
C-T, G-A
d)
A-C, T-C
40.

Place the following in sequential order: 1. Recoil, 2. Straightens the double helix, 3. Breaks Hydrogen bonds, 4. Adds Free nucleotides. The correct sequence is (a)   .

Choose from the below words
1, 2, 3, 4
3, 4, 2, 1
3, 2, 4, 1
2, 3, 4, 1
41.

What is the role of DNA ligase in the replication of DNA?

a)

It helps in unwinding the DNA helix

b)

It joins the Okazaki fragments on the lagging strand

c)

It adds nucleotides to the growing DNA strand

d)

It prevents the reannealing of DNA strands

42.

What is the function of the enzyme primase in DNA replication?

a)

It unwinds the DNA double helix

b)

It synthesizes a short RNA primer

c)

It joins the Okazaki fragments

d)

It adds nucleotides to the growing DNA strand

43.

What is the role of the enzyme helicase in the process of DNA replication?

a)

It synthesizes a short RNA primer

b)

It unwinds the DNA double helix

c)

It joins the Okazaki fragments

d)

It adds nucleotides to the growing DNA strand

44.

What is the function of the enzyme topoisomerase in DNA replication?

a)

It unwinds the DNA double helix

b)

It synthesizes a short RNA primer

c)

It relieves strain in the DNA ahead of the replication fork

d)

It adds nucleotides to the growing DNA strand

45.

Decide if the descriptions best fit DNA, RNA, or both.

Categorize the following

Made of nucleotides

Type of nucleic acid

Single stranded

Short

A, U, C, G

Ribose

Double stranded

Long

A, C, T, G

Deoxyribose

DNA
RNA
Both
46.

If a DNA molecule has 30% Guanine bases, how many Cytosine bases will it have?

a)

30%

b)

25%

c)

20%

d)

10%

47.

If a person suffered from a disorder where their DNA fragments were unable to fill in the missing slots in between the fragments of the lagging strand, they would most likely have a disorder that affected which enzyme?

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase

d)

RNA Polymerase

48.

If a person suffered from a disorder where their DNA fragments were unable to fill in the missing slots in between the fragments of the lagging strand, they would most likely have a disorder that affected which enzyme?

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase

d)

RNA Polymerase

49.

Which letter in the picture below depicts the replication fork in DNA replication?

a)

A

b)

C

c)

B

d)

None of the above

50.

The picture below shows an enzyme unwinding and unzipping DNA.

​What is the name of this enzyme?

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase

d)

RNA Polymerase

51.

The twisted ladder shape of DNA is known as a

a)

Double Helix

b)

Triple Helix

c)

Single Helix

d)

Double felix

52.
Name this structure
a)
double helix
b)

nucleotide

c)
amino acid
d)
protein
53.

A and T are connected by ​ (a)   in DNA and G and C are connected by ​ (b)   .

Choose from the below words
2 hydrogen bonds
3 hydrogen bonds
54.

What is the primary function of DNA polymerase during DNA replication?

a)

To unwind the DNA double helix

b)

To synthesize a new DNA strand by adding nucleotides

c)

To join Okazaki fragments

d)

To prevent DNA strands from reannealing

55.

Which of the following statements about the lagging strand during DNA replication is true?

a)

It is synthesized continuously

b)

It is synthesized in short segments called Okazaki fragments

c)

It is synthesized in the 3' to 5' direction

d)

It requires no RNA primers

56.

What is the role of topoisomerase in DNA replication?

a)

To hold the DNA strands apart

b)

To synthesize RNA primers

c)

To relieve the strain caused by unwinding of the DNA helix

d)

To add nucleotides to the growing DNA strand

57.

Number 1 in the picture above

a)

Replication

b)

DNA Polymerase

c)

Primase

d)

Ligase

58.

#4 in the picture

a)

Polymerase

b)

Primase

c)

Helicase

d)

Ligase

59.

#5

a)

Leading strand

b)

Lagging Strand

c)

Replication fork

d)

Helicase

60.
Which sequence of DNA bases would pair with this partial strand
ATG TGA CAG
a)
ATG TGA CAG
b)
TAC ACT GTC
c)
GTA AGT GAC
d)
CAT TCA CTG
61.

Adenine bonds with​ (a)   and Cytosine bonds with ​ (b)  

Choose from the below words
Thymine
guanine
adenine
cytosine
62.

Match the following

a)

Topoisomerase

1.

Loosens DNA helix

b)

Helicase

2.

Unzips DNA strand

c)

Primase

3.

Starts base pairing and placing Okazaki fragmenst

d)

DNA Polymerase

4.

Adds bases and proofreads strand

e)

Ligase

5.

Reattaches DNA at end of replication

63.

Which is in order from largest to smallest structure?

a)

chromosome, DNA strand, gene, nucleotide

b)

chromosome, gene, DNA strand, nucleotide

c)

gene, DNA strand, chromosome, nucleotide

d)

DNA strand, chromosome, gene, nucleotide

64.
order of replication
a)
3, 2, 1, 4
b)
2, 1, 4, 3
c)
2, 3, 1, 4
d)
3, 4, 1, 2
65.

A segment of a DNA strand is shown. Which of these is the correct complementary DNA strand for the segment shown?

a)

5' AGGTCAGGT 3'

b)

5' ACCUGAGGU 3'

c)

5' TGGACTGGA 3'

d)

5' TCCAGTCCA 3'

66.

Eight components present in nucleic acids are listed in the box. Which components bond with adenine in a section of double-stranded DNA?

a)

1, 3, 5, and 6 only

b)

3 and 5 only

c)

2 and 4 only

d)

3, 4, 7, and 8 only

67.

A student used poster board to construct this model of a section of DNA. Which statement describes this model of DNA?

a)

the model is inaccurate because the base pairs are incorrect

b)

the model is accurate because it contains correctly paired bases

c)

the model is accurate because it shows each base splitting to form a double helix

d)

the model is inaccurate because some typical bases in DNA are missing

68.

A student builds a model of a DNA strand. Which of these models of a DNA strand shows bases that are complementary to the ones on the student's DNA model?

a)
b)
c)
d)
69.

The three components of a nucleotide are​ (a)   , ​ (b)   group, and ​ (c)   base.

Choose from the below words
sugar
phosphate
nitrogenous
70.

A model of a DNA molecule is shown. The arrow indicates -

a)

the bond between adjacent phosphate and deoxyribose molecules

b)

the junction of introns and exons in the sense strand of DNA

c)

the hydrogen bond between complementary nucleotides

d)

the junction of a codon and a DNA triplet