Worksheetshematology- WBCs
Total questions: 38
Worksheet time: 19mins
Leukopenia
increased WBC count
decreased WBC count
leukocytopenia
increased WBC count
decreased WBC count
Leukocytosis
increased WBC count
decreased WBC count
For every 1 WBC there are ____platelets and _____ RBCs
30
500
500
30
30
300
20
380
The leukocyte count is useful to the physician in order to:
detect a specific disease
diagnosis
prognosis
The leukocyte count is useful to the physician in order to:
follow the course of a disease
diagnosis
prognosis
REFERENCE RANGE WBC COUNT
4.00– 11.0 x 103/µL
32-36 x 103/µL
80-100 x 103/µL
10.0- 30.0 x 103/µL
REFERENCE RANGE WBC COUNT (newborns)
4.00– 11.0 x 103/µL
32-36 x 103/µL
80-100 x 103/µL
10.0- 30.0 x 103/µL
Appendicitis, Leukemia, Pregnancy, HDN
INCREASED
WBC count
DECREASED
WBC count
Brucellosis, Typhoid fever, Infectious hepatitis, Rheumatoid arthritis, Lupus, erythromatosus, Cirrhosis
INCREASED
WBC count
DECREASED
WBC count
morning
INCREASED
WBC count
DECREASED
WBC count
afternoon
INCREASED
WBC count
DECREASED
WBC count
bacterial infections
INCREASED
WBC count
DECREASED
WBC count
Viral infections
INCREASED
WBC count
DECREASED
WBC count
fight infection by phagocytosis
Cause leukocytosis
Increased in most bacterial infections
NEUTROPHILS (Segs)
LYMPHOCYTES (Lymphs)
platelets
all of the above
Fight infections by antibody production
Normal count or leukopenia
Increased in viral infections
NEUTROPHILS (Segs)
LYMPHOCYTES (Lymphs)
platelets
all of the above
WBC count is too low for the analyzer to accurately perform the count
use _______
Manual WBC count
estimates performed on the patient’s peripheral blood smear
both
none of the above
WBC count on body fluids, such as CSF, peritoneal, synovial (joint), etc.
use _______
Manual WBC count
estimates performed on the patient’s peripheral blood smear
both
none of the above
WBC count on whole blood with a "normal" specimen
use _______
Manual WBC count
estimates performed on the patient’s peripheral blood smear
both
none of the above
Methods for performing a WBC count estimate from a blood smear.
How many high power fields are counted?
5-10
2-3
10-15
all can be used
Methods for performing a WBC count estimate from a blood smear.
What is the count formula using high power?
Average # WBCs in 5-10 fields x 2,000
Average # WBCs in 5-10 fields x 200
Average # WBCs in 5 fields x 0.2
Methods for performing a WBC count estimate from a blood smear.
What is the count formula using low power in microliters?
Average # WBCs in 5-10 fields x 2,000
Average # WBCs in 5-10 fields x 200
Average # WBCs in 5 fields x 0.2
Methods for performing a WBC count estimate from a blood smear.
What is the count formula using low power in liters?
Average # WBCs in 5-10 fields x 2,000
Average # WBCs in 5-10 fields x 200
Average # WBCs in 5 fields x 0.2
High power when reading a blood smear is _____
100x
40x
10x
all of the above
Low power when reading a blood smear is _____
100x
40x
10x
all of the above
hemacytometer
Reagents (Diluting fluids)
Which is used in the Thrombo-tIC?
1% HCL
2% Acetic acid
Turk’s (acetic acid+ gentian violet)
ammonium oxalate
all of the above
hemacytometer
Reagents (Diluting fluids)
Which lyze RBCs?
1% HCL
2% Acetic acid
Turk’s (acetic acid+ gentian violet)
ammonium oxalate
all of the above
TYPES OF HEMACYTOMETERS
depth of 0.1 mm
used routinely to count manual cell counts
Spencer Bright Line (Improved Neubauer)
Fuchs-Rosenthal
TYPES OF HEMACYTOMETERS
depth of 0.2 mm
May be used to count spinal fluid cells
Spencer Bright Line (Improved Neubauer)
Fuchs-Rosenthal
SOURCES OF ERROR Manual WBC/Platelet Counts
improper mix of EDTA sample
improper filling of hemacytometer
Counting cells twice
all of the above
Which is incorrect about cleaning a hemacytometer?
Dry all areas (even the ruled areas) with kimwipes.
dip in 10% chlorox., then water then, then methanol
Dry and clean the ruled areas and the coverslip with lens paper only
all are correct
# Cells/mm3 = DF x Depth Factor x average # cells
average area counted (mm2)
What is the depth factor?
10
reciprocal of the dilution
given in the counts
an average
Uncorrected WBC x 100
100 + nRBCs (per 100 WBC)
The formula is used _______
when 5 or more nucleated RBCs are seen on the blood smear differential
to calculate the WBC count manually
to estimate the WBC count from a blood smear
to preform a Leuko-tic cell count
Which is false of the Leuko-tic Method?
Uses dark field electroscopy
Turk’s solution (acetic acid) lyzes RBC
Gentian violet will stain the WBC nucleus at violet-blue
A 1: 20 dilution is used
20µL whole blood
380 µl diluent
The relative WBC count is the percentage of each type of WBC counted, adds up to 100%. For instance, 55% are ____
Neutrophils(Segs)
Lymphocytes(Lymphs)
Monocytes(Monos)
all WBCs
The relative WBC count is the percentage of each type of WBC counted, adds up to 100%. For instance, 40% are ____
Neutrophils(Segs)
Lymphocytes(Lymphs)
Monocytes(Monos)
all WBCs
The relative WBC count is the percentage of each type of WBC counted, adds up to 100%. For instance, 5% are ____
Neutrophils(Segs)
Lymphocytes(Lymphs)
Monocytes(Monos)
all WBCs
According to the reference values, how many monocytes should be found in a 100 WBC count?
2-10
0-900
<5
0-0.9
