WorksheetsSTAPH and MICROCOCCACEAE ID
Total questions: 22
Worksheet time: 13mins
Which are biochemical result for Staph?
Glucose oxidizer
Gluc. fermenter
Bacitracin S
Bacitracin R
Which are biochemical result for Micrococcus?
Facultative Yes
Facultative No
Lysostaphin S
Lysostaphin R
Which are biochemical result for Staph?
Facultative Yes
Facultative No
Lysostaphin S
Lysostaphin R
Which are biochemical result for Micro?
Glucose oxidizer
Gluc. fermenter
Bacitracin S
Bacitracin R
biochemical ID matching
Coagulase -
Growth on MSA &
Mannitol -
Novobiocin S (urine)
Stap. aureus
Stap. Sapro
Stap. Epi
Micrococcus ssp.
STAPH AUREUS ANTIBIOTIC RESISTANCE includes sensitivity to _____
Penicillin G
ampicillin
cephalosporins
all of the above
Which is the enzyme tested in S. aureus to determine sensitivity to penicillin G?
B-lactamase
Coagulase
Hyaluronidase
Nucleases (DNAse)
When in NOT a chemical toxin released by S. aureus?
Enterotoxin
Exfoliatin/ Leukocidin
TSST (Toxic Shock Syndrome Toxin)
Fibrinolysin
How do you determine STAPH AUREUS ANTIBIOTIC RESISTANCE?
Kirby Bauer testing
Rapid chromagenic cephalosporin test
enzyme tetstng for attacks on B-lactam ring
ChromAgar Screen
What is the testing method to determine MERSA strains of S. aureus?
ChromAgar Screen
Oxacillin screen Kirby-Bauer
Rapid chromagenic cephalosporin test
toxin testing methods
Which is NOT specific to the Oxacillin screen Kirby-Bauer for MERSA?
incubate full 24 hrs
look for mauve colonies or Denim blue colonies
Media with increased NaCl
incubate no higher than 35oC
Purpose: Screen for MRSA carriers
Specimen: Anterior nares/other
Media: contains Cefoxitin, other inhibitors and a chromagenic substrate
CHROMagar Screen
BACITRACIN (TAXO A)SUSCEPTIBILITY
CAMP TEST
SODIUM HIPPURATE TEST
Principle: MRSA will produce an enzyme that hydrolyzes the chromagenic substrate, resulting in mauve-colored colonies or denim blue colonies—depends on media/company used
CHROMagar Screen
BACITRACIN (TAXO A)SUSCEPTIBILITY
CAMP TEST
SODIUM HIPPURATE TEST
STEPS:
Inoculate Media/Streak for isolation
Incubate in the dark in air at 35-370C for 24 hrs.
(+/- 4 hrs.)
Observe for denim blue or mauve colonies, produced by MRSA
CHROMagar Screen
BACITRACIN (TAXO A)SUSCEPTIBILITY
CAMP TEST
SODIUM HIPPURATE TEST
Which is true of VRSA?
Vancomycin Resistant Staph aureus
found in all Stap. ssp
Resistance to penicillin G
MERSA Stap aureus
biochemical ID matching
Coagulase -
Growth on MSA &
Mannitol +/-
Novobiocin R
Stap. aureus
Stap. Sapro
Stap. Epi
Micrococcus ssp.
biochemical ID matching
Coagulase +
Mannitol Salt (MSA) +
DNase/Thermo-nuclease +
Stap. aureus
Stap. Sapro
Stap. Epi
Micrococcus ssp.
gpc, pairs & clusters
(tetrads)
Coag negative Staph & Micrococcus
Alpha strep (strep viridans)
Corynebacterium (diphtheroids)
Yeast
Propionibacterium
gpc, pairs and chains
Coag negative Staph & Micrococcus
Alpha strep (strep viridans)
Corynebacterium (diphtheroids)
Yeast
Propionibacterium
Pleomorphic gpb
Coag negative Staph & Micrococcus
Alpha strep (strep viridans)
Corynebacterium (diphtheroids)
Yeast
Propionibacterium
gram positive to gram variable, round to oval cells
Coag negative Staph & Micrococcus
Alpha strep (strep viridans)
Corynebacterium (diphtheroids)
Yeast
Propionibacterium
(anaerobe): pleomorphic gpb
Coag negative Staph & Micrococcus
Alpha strep (strep viridans)
Corynebacterium (diphtheroids)
Yeast
Propionibacterium
