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Worksheets

Transcription & Translation

Total questions: 55

Worksheet time: 37mins

Name
Class
Date
1.
How does RNA differ from DNA?
a)
RNA contains uracil and deoxyribose
b)
RNA contains ribose and thymine
c)
RNA contains uracil and ribose
d)
RNA contains adenine and ribose
2.
How would the DNA sequence GCTATA be transcribed to mRNA?
a)
GCUAUA
b)
CGATAT
c)
CGAUAU
d)
GCUTUT
3.

What is the correct order to make a protein?

a)

Protein, DNA, RNA

b)

RNA, DNA, Protein

c)

DNA, RNA, Protein

d)

Protein, RNA, DNA

4.

RNA has what base that is instead of thymine?

a)

guanine

b)

adenine

c)

thymine

d)

uracil

5.

What enzyme does transcription use?

a)

DNA polymerase

b)

ligase

c)

RNA polymerase

d)

helicase

6.

Which RNA carries the message?

a)

mRNA

b)

rRNA

c)

tRNA

7.
What part of a nucleotide accounts for the genetic variation between individuals?
a)
phosphate
b)
nitrogenous base
c)
deoxyribose
d)
sugar
8.
If one strand of DNA has the sequence TTACCG, the sequence of the other strand of the same molecule will be
a)
AATGGC
b)
TTACCG
c)
AACGGC
d)
AATGGG
9.
Where does transcription take place?
a)
nucleus
b)
cytoplasm
c)
ribosome
10.

DNA contains the template to make RNA

a)

True

b)

False

11.

Where are proteins made?

a)

ER

b)

nucleus

c)

cytoplasm

d)

mitochondria

12.
What is occurring in this picture?
a)
Translation
b)
Replication
c)
Translocation
d)
Transcription
13.
One difference between DNA and RNA is that....
a)
Only RNA has the base Uracil
b)
only RNA contains the base Thymine
c)
only RNA contains the base Cytocine
d)
only RNA contained the base Guanine
14.
True or false:  RNA is double-stranded.
a)
true
b)
false
c)
it depends on the type
15.
Which of these bases does not exist in RNA?
a)
C
b)
T
c)
U
d)
A
16.
Which RNA bases would pair with TACGAA in transcription
a)
AUGCUU
b)
ATGCTT
c)
GCATCC
d)
GCAUCC
17.
DNA codes for...
a)
cholesterol
b)
proteins
c)
fatty acids
d)
a sandwich
18.

RNA A pairs with?

a)

g

b)

4

c)

u

d)

t

19.

The process of making RNA from DNA is called

a)

Replication

b)

Transcription

c)

Translation

d)

Reversetranscription

20.

The______ region lies towards the 3'end of the coding sequence

a)

Promoter

b)

Terminator

c)

Regulator

d)

Structural genes

21.

________ initiates the process of transcription in prokaryotes

a)

Sigma factor

b)

Rho factor

c)

Epsilon factor

d)

Gamma factor

22.

Binding of rho factor to ______ releases the RNA transcript and braking of transcriptional assembly

a)

Promoter

b)

Terminator

c)

Operator

d)

Regulator

23.

hm RNA is transcribed by ______ in eukarotes

a)

RNA polymerase I

b)

RNAPolymersaIi

c)

RNA polymerase III

d)

Reverse transcriptase

24.

t RNA is transcribed by

a)

RNA polymerase I

b)

RNA polymerase II

c)

RNA polymerase III

d)

None

25.

5S rRNA is transcribed by

a)

RNA polymerase I

b)

RNA polymerase II

c)

RNA polymerase III

d)

DNA polymerse

26.

Removal of noncoding sequences from hn RNA is called

a)

Capping

b)

Splicing

c)

Tailing

d)

Spooling

27.

Capping is addition of _____ towards 5' end of hn RNA

a)

Methyl cytosine

b)

Methyl guanosine

c)

Methyl adenosine

d)

Methyl uridine

28.

UTR stands for

a)

Untranscribed regions

b)

Untranslated regions

c)

Untagged regions

d)

Untailed regions

29.

Tailing refers to addition of ________ towards the 3' end of hn RNA

a)

Poly adenylate residues

b)

Poly guanylate residues

c)

Polycytidylate residues

d)

None

30.

Removal of noncoding sequences from hn RNA is called

a)

Capping

b)

Splicing

c)

Tailing

d)

Spooling

31.

Capping is addition of _____ towards 5' end of hn RNA

a)

Methyl cytosine

b)

Methyl guanosine

c)

Methyl adenosine

d)

Methyl uridine

32.

UTR stands for

a)

Untranscribed regions

b)

Untranslated regions

c)

Untagged regions

d)

Untailed regions

33.

The enzyme RNA polymerse joins to the _______ site of transcriptional assembly

a)

Regulator

b)

Operator

c)

Promoter

d)

Terminator

34.

In splicing, _________ are removed and __________ are connected.

a)

Introns; exons

b)

Caps; tails

c)

Exons; introns

d)

Sigma factors; nucleotides

35.

Transcription occurs in the _______ of prokaryotes, and in the _________ of eukaryotes.

a)

Cytoplasm ; cytoplasm

b)

Nucleus ; ribosome

c)

Nucleolus ; nucleus

d)

Cytoplasm ; nucleus

e)

Nucleoid ; cytoplasm

36.

What is the central dogma?

a)

DNA --> mRNA --> tRNA --> rRNA

b)

Template strand --> coding strand --> mRNA

c)

Nucleus --> cytoplasm --> ribosome

d)

DNA --> mRNA --> protein

37.

In prokaryotes, the ________ delivers the ____________ to the promotor region of the DNA.

a)

RNA Polymerase; ribosome

b)

sigma factor; RNA Polymerase

c)

Splisosome; sigma factor

d)

Sigma factor; mRNA

38.

Given the coding strand, what is the template strand?

5'- AGCTTA -3'

a)

5'- ATTCGA -3'

b)

5'- UCGAAU -3'

c)

5'- TAAGCT -3'

d)

5'- TCGAAT -3'

39.

Transcription takes place in the ......

a)

Ribosome

b)

Nucleus

c)

Cytoplasm

d)

Vacuole

40.

When separating the DNA strand, the _______ bonds between the bases are split.

a)

Covalent

b)

Hydrogen

c)

Ionic

41.

What is the matching mRNA strand to this DNA strand?

TAC TTT CCA

a)

AUG AAA GGU

b)

ATG AAA GGT

c)

UTG UUU GGT

42.

What does DNA have that RNA doesn't

a)

Presence of thymine

b)

Presence of deoxyribose

c)

Presence of ribose

d)

Both a and b

43.
Which enzyme "glues" DNA fragments together?
a)
restriction enzymes
b)
DNA ligase
c)
DNA polymerase
d)
DNA helicase
44.
Restriction enzymes....
a)
Cut DNA at a specific site
b)
Can be used to isolate a gene of interest
c)
Can be used to cut vector DNA
d)
Do all of these things
45.

Adding of methyl guanosine to m rna is called

a)

Capping

b)

Trailing

c)

Tailing

d)

All

46.

Why are introns removed

a)

They have no function

b)

They are intervening sequence

c)

They do not code for Protein

d)

All

47.

Given the template strand, what is the mRNA strand?

5'- GATGAAC -3'

a)

5'- GUUCAUC -3'

b)

5'- CUACUUG -3'

c)

5'- GAACUAC -3'

d)

5'- GTTCATC -3'

48.

In splicing, _________ are removed and __________ are connected.

a)

Introns; exons

b)

Caps; tails

c)

Exons; introns

d)

Sigma factors; nucleotides

49.

In prokaryotes, the ________ delivers the ____________ to the promotor region of the DNA.

a)

RNA Polymerase; ribosome

b)

sigma factor; RNA Polymerase

c)

Splisosome; sigma factor

d)

Sigma factor; mRNA

50.

What is the function of RNA polymerase?

a)

To recognize the promotor region

b)

To recognize the transcribed region

c)

To synthesize RNA

d)

To hydrolyze RNA

e)

To "read" the coding strand

51.

Which of the following are exceptions to the central dogma? Select all that apply!

a)

DNA --> mRNA

b)

DNA --> tRNA

c)

DNA --> rRNA

d)

RNA --> DNA (retroviruses)

52.

Which of the following is complementary and antiparallel to the template strand? Select all that apply!

a)

The non-coding strand

b)

The coding strand

c)

The polypeptide

d)

The tRNA

e)

The mRNA

53.

Ture or false: The terminator is transcribed by RNA Polymerase.

a)

True

b)

False

54.

The protein and RNA complex that removes introns and connects exons is called the _______.

a)

Sigma factor

b)

Open complex

c)

RNA polymerase

d)

Spliceosome

e)

Cap and tail

55.

The cap goes on the ________ end of the mRNA, and the poly-A tail goes on the ______ end.

a)

Hairpin ; open

b)

5' ; 3'

c)

3' ; 5'

d)

Open ; hairpin