Worksheets11 ATAR HB - Microscopy
Total questions: 28
Worksheet time: 44mins
When looking through your microscope, if you move your slide to the right the slide appears
to move to the right
to stay the same
to move to the left
vanish
Use the _______ focuses the image under low power.
revolving nosepiece
fine adjustment knob
coarse adjustment knob
arm/neck
The __________ sharpens the image under high magnification.
the diaphragm
fine adjustment knob
coarse adjustment knob
the ocular
The ________ of the microscope would give you the SMALLEST field of view.
low power objective
medium power objective
high power objective
stage
Eyepiece = 10X
Objective lens = 4X
Eyepiece = 20X
Objective lens = 20X
Eyepiece = 15X
Objective lens = 100X
Which way should you move the slide to center the cheek cell?
to the right
to the left
up (away from you)
down (toward you)
The largest field of view shown is under the total magnification _____X.
40
100
400
Ocular Lens: 10X
Scanning Lens: 4X
Low Power Lens: 10X
High Power Lens: 42X
F.O.V for 4X = 3.4 mm
Use this information to figure out big an average bacteria is if 256 fit across the field of view of the high power objective.
Ocular Lens: 10X
Scanning Lens: 4X
Low Power Lens: 15X
High Power Lens: 50X
F.O.V for 4X = 3.4 mm
You are viewing human blood under low power. How much bigger do those cells appear to you through the eyepiece. (compared to what they are in real life)
Ocular Lens: 10X
Scanning Lens: 4X
Low Power Lens: 10X
High Power Lens: 40X
F.O.V for 4X = 3.4 mm
You are viewing human blood under high power. You need to calculate the average size of a red blood cell. You estimate that 51 cells will fit across your F.O.V. What is the average size of one of those red blood cells?
Ocular Lens: 10X
Scanning Lens: 4X
Low Power Lens: 15X
High Power Lens: 50X
You measure the field of view under scanning power as 4.8 mm. Calculate (in micrometers) the field of view that would appear on low power?
Ocular Lens: 10X
Low Power Lens: 20X
High Power Lens: 50X
You measure the field of view under low power to be 2.6 mm
Calculate (in micrometers) the field of view that would appear on high power.
What is the other name of the eyepiece?
Objective
Ocular
Scanner
Viewer
If the ocular has a magnification of 10x and the low power objective is also 10x, what is the total magnification?
(a)
If the ocular has a magnification of 10x and the high power objective is also 40x, what is the total magnification?
(a)
Adding iodine or methylene blue on a slide to enhance the organelles of a specimen is called __________.
marking
staining
labelling
labelling
Why do you place the coverslip at a 45 degree angle before allowing it to cover the specimen?
To center the specimen under the coverslip.
To push air bubbles out as the coverslip falls.
To reduce the risk of breaking the slide.
To make sure the specimen does not get smashed.
Which objective lens has the smallest field of vision?
scanner
low-power
high-power
ocular
Which is not a step in preparing a wet mount?
Clean the slide to remove previous residue.
Place the specimen in the center of a drop of water.
Drop the coverslip straight down on the specimen.
Tap out any bubbles using the back of a probe.
These cells are
Elodea
Anacharis
Cheek
Onion
These cells are
Elodea
Anacharis
Cheek
Onion
What stain was used on these cells.
Water
Iodine
Methylene Blue
Bleach
