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Chapter 4: Biocatalysis

Total questions: 20

Worksheet time: 15mins

Name
Class
Date
1.

Enzymes are composed of what organic molecule?

a)

Sugars

b)

DNA

c)

Proteins

d)

Fatty Acids

2.

What happens to an enzyme when it is denatured?

a)

Activation energy is lowered.

b)

It becomes stronger.

c)

It loses its shape.

d)

Activation energy is raised.

3.

Based on the graph, what temperature does this enzyme work best?

a)

15 C

b)

40 C

c)

30 C

d)

20C

4.

In the diagram, what is letter A?

a)

product

b)

substrate

c)

enzyme-substrate complex

d)

active site

5.

.

Which line represents an enzyme-catalyzed reaction?

a)

Line 1

b)

Line 2

6.

In a chemical reaction, a reactant binds to an enzyme at a region known as the

a)

catalyst.

b)

product.

c)

substrate.

d)

active site.

7.

Significantly changing the pH, the enzyme is exposed to will cause the enzyme to _____________.

a)

increase enzymatic action

b)

fit better with the substrate

c)

denature

d)

evaporate

8.

the amount of energy needed to start the reaction

a)

cofactor

b)

activation energy

c)

catalyst

d)

specificity

9.

The suffix found in names of enzymes; all enzymes end in this suffix

a)

-ise

b)

-ase

c)

-ose

d)

-ame

10.

Which of the following conclusions can be drawn from this graph?

a)

The optimum pH of the enzyme is 6.6.

b)

The optimum pH of the enzyme is 5.8

c)

The enzyme’s activity increases as pH increases 5.0 to 9.0

d)

The enzyme’s activity is greater around pH of 8.0 .

11.

Which of these is most likely an enzyme?

a)

Sucrose

b)

Galactose

c)

Fructose

d)

Amylase

12.

Which cofactors are not permanently bound to enzymes?

a)

Metal Ion cofactors

b)

prosthetic groups

13.

Some cofactors change the charge distribution on the surface of substrate or enzyme molecules. Suggest what importance this has?

a)

Makes the temporary bonds of ES complexes easier to form

b)

Makes the permanent bonds of ES complexes easier to form

c)

Makes it easier to form EP complexes

d)

Makes it easier for products to bind to the active site

14.

State the function of X?

a)

Binding site of substrate

b)

Binding site of cofactor

c)

Binding site of reactant

d)

Binding site of inhibitor

15.

Enzyme that join the ends of two strands of nucleic acid is

a)

Ligases

b)

Hydrolases

c)

Transferases

d)

Isomerases

16.

Which of these statements are true about competitive inhibitor?

a)

Inhibitor and substrate has similar structure

b)

The inhibitor binds to the allosteric site

c)

The conformation of active site changed due to the inhibitor

d)

Quantity of product formed is the same as reaction with inhibitor

17.

.

Which of these are competitive inhibitor?

answer choices

a)

Hydrogen cyanide

b)

Malonic acid

c)

Mercury

d)

Isoleucine

18.

Give one similarities between competitive inhibitor and non-competitive inhibitor action

a)

Both caused enzyme's conformation changed

b)

The maximum rate of reaction is not reached

c)

Reaction becomes slower

d)

The rate of reaction can be increased by increasing the substrate concentration

19.

what impedes enzymatic reactions by binding to another part of the enzyme?

a)

noncompetitive inhibitor

b)

competitive inhibitors

c)

coenzymes

d)

cofactors

20.

Competitive inhibition can be overcome by _______________

a)

increase the concentration of substrate

b)

reduce the concentration of enzyme

c)

increase the concentration of enzyme

d)

reduce the concentration of substrate