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WorksheetsMircobiol Mid Term
Total questions: 157
Worksheet time: 3hrs 30mins
Our Microbiology Lab (here at TWU) is classified at what Biosafety Level?
(a)
The word that describes how microorganisms are found nearly everywhere in the environment is:
(a)
The word that describes how microorganisms are found nearly everywhere in the environment is:
Plentiful
Ubiquitous
Abundant
Profuse
True or False
You Can Wear Crocs to Lab!
True
False
Name ONE potential route of exposure.
(a)
Which is NOT an example of an organism with a Biosafety level of 2?
Clostridium botulinum
Bacillus subtilus
Staphylococcus aureus
Clostridium tetani
Pseudomonas aeruginosa
True or False
Ebola is a Biosafety Level 2 Organism
True
False
What is the correct order of events when there was a splash and bacterial culture makes contact with an open wound?
1) Inform the instructor that the bacteria made contact to your skin
2) Wash wound thoroughly with soap and water
3) Tell others there was a splash
1) Tell others there was a splash
2) Inform the instructor that the bacteria made contact to your skin
3) Wash wound thoroughly with soap and water
1) Wash wound thoroughly with soap and water
2) Inform the instructor that the bacteria made contact to your skin
3) Tell others there was a splash
The most defining attribute for the organisms studied in microbiology is that they are too _________ to be seen with the naked eye.
big
colorful
small
irregular shaped
An _______ microscope can be used to detect tiny viruses and even molecules!
atomic
neuron
electron
photon
What is the total magnification when using a 4X objective lens?
400
400X
40
40X
What is the total magnification when using a 10X objective lens?
(a)
The _______ ________ (2 words) of a microscope is the ability to distinguish close adjacent objects as being separate objects
(a)
Label A
(a)
Label B
(a)
Label C
(a)
Label D
(a)
Label E
(a)
Label F
(a)
Label G
(a)
Label H
(a)
Label I
(a)
Label J
(a)
Label K
(a)
Label L
(a)
Label M
(a)
Label N
(a)
Label O
(a)
Label P
(a)
Label Q
(a)
Label R
(a)
The area on the slide one can observe while looking through the oculars is called the ________ _______ _________ _______ (4 words).
(a)
The _________ _________ (2 words) of a microscope is the ability to distinguish close adjacent objects as being separate objects.
(a)
________ ________ (2 words) is the distance separating a specimen and the objective lens.
(a)
When the microscope only needs minor focusing adjustments after the specimen is found and focused and focused using the lowing power is called (a) .
True or False
When beginning to look as a slide, you should start on the lowest objective with the stage at the highest position.
True
False
What is this underneath the microscope?
stained bacteria
blood-yeast-bacteria
yeast cells
pond water
The three domains of life are _________, __________, and ___________.
(type as: ANSWER, ANSWER, ANSWER or the computer will mark it wrong)
(a)
What microorganism matches the following characteristics:
Extremely small bacteria that cannot be cultured using ordinary microbiological methods.
(a)
What microorganism matches the following characteristics:
These grow as either sporophytes in nature or as parasites in susceptible hosts.
(a)
What microorganism matches the following characteristics:
Obligate intracellular parasites that are not true cells. Classified based on physical attributes like shape.
(a)
What microorganism matches the following characteristics:
The largest true unicellular microbe. Classified based on their locomotory structures.
(a)
What microorganism matches the following characteristics:
Worms that are soft-bodied invertebrates in Kingdom Animalia. Two major groups: Roundworms and Flatworms.
(a)
What microorganism matches the following characteristics:
Aquatic organisms that produce oxygen as a byproduct of photosynthesis.
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
What TYPE of microorganism is this?
(a)
Any unintentional organism that is introduced into a culture is a (a) .
When we introduce an inoculum into a nutrient medium that provides a suitable environment to reproduce, this process is called:
(a)
When we introduce an inoculum into a nutrient medium that provides a suitable environment to reproduce, this process is called:
Inoculation
Pure Culture
Mixed Culture
Sample Culturing
When we culture an organism, we introduce a sample, otherwise known as the ___________, into a container of nutrient medium.
sample aliquot
pipette transfer
inoculum
sample transfer
When we culture an organism, we introduce a sample, otherwise known as the (a) , into a container of nutrient medium.
What are one of the six basic nutritional requirements and these are incorporated into the media to ensure the microorganisms can grow?
One of them are: Trace Elements
(a)
Which is NOT one of the six basic nutritional requirements and these are incorporated into the media to ensure the microorganisms can grow?
oxygen
lipids
proteins
vitamins
What are the six basic nutritional requirements and these are incorporated into the media to ensure the microorganisms can grow?
water, oxygen, energy, amino acids, protein, energy
sunlight, carbohydrates, proteins, vitamins, lipids, nitrogen
water, carbohydrates, proteins, vitamins, lipids, trace elements
water, carbohydrates, proteins, energy, lipids, trace elements
Which quadrant should you find separated colonies?
(a)
What order should you streak the plate?
A -> D-> C -> B
A -> B -> C -> D
B -> A -> C -> D
C -> B -> A -> D
Bacteria, viruses, and other microbes that cause disease are called (a) .
_________ _________ (2 words) means following specific practices and procedures that prevent contamination with microorganisms.
(a)
You should flame the loop until it is...
(a)
(a) are inanimate objects that can become contaminated with infectious agents and serve as a mechanism for transfer between hosts.
Bacteria, yeast, or mold?
Bacteria
Yeast
Mold
What is this on the agar plate?
(a)
Bacteria, yeast, or mold?
Bacteria
Yeast
Mold
Bacteria, yeast, or mold?
Bacteria
Yeast
Mold
Bacteria, yeast, or mold?
Bacteria
Yeast
Mold
What is this on the agar plate?
(a)
What is this on the agar plate?
(a)
What is this on the agar plate?
(a)
What is shape #1 called?
(a)
What is shape #2 called?
(a)
What is shape #3 called?
(a)
What is shape #4 called?
(a)
What is shape #5 called?
(a)
What is margin #1 called?
(a)
What is margin #2 called?
(a)
What is margin #3 called?
(a)
What is margin #4 called?
(a)
What is margin #5 called?
(a)
What is margin #6 called?
(a)
What is elevation #1 called?
(a)
What is elevation #2 called?
(a)
What is elevation #3 called?
(a)
What is elevation #4 called?
(a)
What is elevation #5 called?
(a)
What is size #1 called?
(a)
What is size #2 called?
(a)
What is size #3 called?
(a)
What is size #4 called?
(a)
When working with microorganisms you must work with a ______ ______ ( 2 words).
Culture
Pure Culture
Mixed Culture
Inoculate
Typically samples that are taken will be ________ _______ (2 words).
(a)
Any unintentional organism that is introduced into a culture is a (a) .
A samples with only one organism
(a)
A sample with more than two organisms
(a)
True or False
Media can be liquid
True
False
Organisms grown in broth cultures causes what?
Clear
Turbidity
Crystallization
Organisms grown in broth cultures causing it to become cloudy.
(a)
What is the most common solidifying material used in solid media?
(a)
Media may be _______ (broth) or ________.
Write answer: ANSWER or ANSWER
(a)
The _________ __________ ________ _______ (4 words) allows bacterial cells to be widely separated on agar so that as the single cell divides it will produce a visible mass (colony).
(a)
What is the process for the streak plate method to isolate cultures?
Hairy or thread like fungi
(a)
_________ ________ (2 words) use only one dye and dye all cells and structures the same color.
(a)
__________ __________ (two words) use two or more stains and give one the capability to categorize cells into groups.
(a)
Gram Positive or Gram Negative?
(a)
Gram Positive or Gram Negative?
(a)
What are the 8 steps involved in the Gram Stain process in the proper staining method order (including water rinses)
A _______ stain is one where ONE dye is used and it reveals shape, size, and arrangement.
special
simple
differential
A (a) stain is one where ONE dye is used and it reveals shape, size, and arrangement.
A __________ stain uses a primary stain and a counterstain to distinguish cell types or part of a cell.
special
simple
differential
A (a) stain uses a primary stain and a counterstain to distinguish cell types or part of a cell.
A ________ stain reveals certain cell parts not revealed with conventional staining methods.
special
simple
differential
A (a) stain reveals certain cell parts not revealed with conventional staining methods.
What stain is this microscopic image present?
(a)
What stain is this microscopic image present?
(a)
What stain is this microscopic image present?
(a)
What stain is this microscopic image present?
(a)
What stain is this microscopic image present?
(a)
What stain is this microscopic image present?
(a)
What stain is this microscopic image present?
(a)
What stain is this microscopic image present?
(a)
What stain is this microscopic image present?
(a)
Gram Positive or Gram Negative - Bacilli
(a)
Gram Positive or Gram Negative - Bacilli
(a)
Gram Positive or Gram Negative - Bacilli
(a)
Gram Positive or Gram Negative - Cocci
(a)
Gram Positive or Gram Negative - Cocci
(a)
What shape is #1 ?
(a)
What shape is #2 ?
(a)
What shape is #3 ?
(a)
What shape is #4 ?
(a)
What shape is #5 ?
(a)
What shape is #1 ?
(a)
What shape is #2 ?
(a)
What shape is #3 ?
(a)
What shape is #4 ?
(a)
What shape is #5 ?
(a)
What shape is #1 ?
(a)
What shape is #2 ?
(a)
What shape is #3 ?
(a)
Bacteria without flagella
(a)
flagella all around
(a)
tuft of many flagella at one end or both ends
(a)
single flagellum
(a)
flagella at both ends
(a)
CFU means...
(a)
