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Worksheetsbiochem quiz 5
Total questions: 15
Worksheet time: 8mins
The name for most enzymes ends in the suffix '______'.
-ate
-ite
-ide
-ase
-ose
The name given to the agent that is chemically modified during an enzyme catalyzed reaction is the ______.
substrate
coenzyme
reaction product
enzyme substrate complex
cofactor
The kinetic performance of an enzyme catalyzed reaction is assessed by preparing a graph of ______ (x-axis) vs. ______ (y-axis).
time -- [S]
Vmax -- time
reaction velocity -- Km
[S] -- reaction velocity
Km -- Vmax
At elevated temperatures (> 45 °C) the catalytic effect of an enzyme ______ due to ______.
increases -- peptide bond hydrolysis
decreases -- rupture of disulfide linkages
increases -- excessive hydrogen bond formation
decreases -- secondary structure modification
decreases -- protein denaturation
The parameter Km measures this property of an enzyme catalyzed reaction.
Vmax
k2/k1
enzyme-substrate affinity
stability of the enzyme substrate complex
substrate concentration
A possible name for the enzyme that catalyzes the reaction: pyruvate + CO2 → oxaloacetate; would probalbly be named ______.
oxaloacetate carboxylase
carbon dioxide dehydrogenase
pyruvate carboxylase
oxaloacetate decarboxylase
pyruvate decarboxylase
An enzyme modifies a chemical process by ______ of the reaction.
decreasing the energy change
increasing the energy change
increasing the rate
increasing the temperature
decreasing the rate
As the temperature of the system increases, enzyme catalyzed reactions often show a(n) ______ in reaction rate as a result of protein ______ due to the increase in temperature.
increase -- hydrolysis
decrease -- hydrolysis
increase -- denaturation
decrease -- denaturation
increase -- precipitation
For the reaction: pNPP + H2O → pNP + Pi; the substrate is ______. NOTE: pNPP = para-nitrophenol phosphate; pNP = para-nitrophenol.
pNP
H2O
Pi
pNPP
Both pNP and pNPP are correct
The greater the affinity of an enzyme for its substrate, the ______ for the system.
larger the Vmax
smaller the Vmax
the larger Km
the smaller the Vmax/2
smaller the Km
An enzyme modifies the ______ of a chemical reaction.
equilibrium constant
temperature
rate
enthalpy change
both "c" and "d" are correct
The activity of an enzyme catalyzed process is dependent upon all of the features listed below except ______.
temperature
pH
type of linkage
substrate concentration
amount of enzyme
3. Km is a measure of the ________________.
affinity of an enzyme for a substrate
oxidation state of an enzyme
tertiary structure of an enzyme
conformation of an enzyme
maximum velocity of the process
A Lineweaver-Burk plot will yield a value for the __________ in the enzyme catalyzed process.
VMax only
velocity of the reaction step: E + P ES
Km only
energy change
both "a" and "c" are correct
Phosphatase enzymes catalyze the ____________ an enzyme.
addition of a phosphate group to
removal of a phosphate group from
reduction of the phosphorus component of
modification of the coenzyme
oxidation of the phosphorus component of
