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Forensic DNA

Total questions: 52

Worksheet time: 31mins

Name
Class
Date
1.

A nucleotide is composed of

a)

sugar ribose

b)

a nitrogen base

c)

a phosphate group

d)

none of the above

e)

all of the above

2.

which of the following are nitrogen bases within the strands of DNA?

a)

thyroxin

b)

guanine

c)

tyrosin

d)

adrenaline

e)

lysine

3.

The complemintary nucleotides in both DNA strands are bonded with :

a)

nitrogen bonds

b)

hydrogen bonds

c)

none of them involved

4.

________ _______ can be thought of as highly specialized scissors that cut a DNA molecule when it recognizes a specific sequence of bases.

a)

digestive enzymes

b)

histolic enzymes

c)

restriction enzymes

d)

none of the above is correct

5.

Refer to the figure, the process A called ______ , and the suspect number ____ is the criminal.

a)

gel electrophoresis / 1

b)

slicing DNA / 2

c)

DNA replication / 1

d)

gel electrophoresis / 2

6.

These are laboratory-made fragments of DNA that are complementary to loci that we desire to be amplified (copied).

a)

alleles

b)

genes

c)

primers

d)

heterozygotes

7.

This is a technique for separating molecules (in this case, DNA fragments) based on their size.

a)

PCR (Polymerase Chain Reaction)

b)

STR (Short Tandem Repeats)

c)

electrophoresis

d)

exemplifying

8.

DNA has what charge?

a)

negative

b)

positive

c)

neutral

d)

electrified

9.

This is the database maintained by the FBI that contains DNA profiles

a)

CODIS

b)

AFIS

c)

IBIS

d)

CARES

10.
The purpose of PCR
a)
cutting DNA
b)
creating RFLPs
c)
amplifying/copy DNA
d)
reversing the charge of DNA
11.
If base-pairing rules state that Adenine always pairs with Thymine, and Cytosine pairs with Guanine, what would be the complementary strand to ACGGT?
a)
ACGGT
b)
TGGCA
c)
TGCCA
d)
ACCGT
12.
What enzyme is used during PCR to pair complementary bases to the template strand?
a)
Thermonuclease
b)
Helicase
c)
DNA Polymerase
d)
RNA Polymerase
13.
How do scientists cut DNA into smaller strands?
a)
restriction enzymes
b)
scissors
c)
ligase
d)
agarose
14.
Based on these results whose blood was found in the blood stain at the crime scene?
a)
Bob
b)
Sue
c)
John
d)
Lisa
15.
The computerized database used to store DNA information is: 
a)
AFIS
b)
NIBIN
c)
CODIS
d)
PDQ
16.
Biologists need additional copies of a DNA fragment. What tool should they use?
a)
Gel Electrophoresis
b)
PCR
c)
Restriction enzymes
d)
This isn't possible
17.
Pattern of DNA fragments obtained by examining a person's unique sequence of DNA base pairs is?
a)
PCR
b)
Chromosome
c)
Electrophoresis
d)
DNA Fingerprinting
18.
Which DNA strands move the fastest through the gel when the electricity is turned on?
a)
The long strands
b)
The short strands
c)
The medium strands
d)
They all move the same
19.
Mr. Jones was killed in a car accident.  Afterwards, a former girlfriend filed a paternity suit claiming that Mr. Jones was the father of her child, and thus she was entitled to the man’s estate.  Which sample of DNA would have to be from Mr. Jones if he were the child’s father? 
a)
2
b)
3
c)
4
d)
5
20.
A boy moves to another country to live with his father.  Years later, the boy decides to move back to live with the woman who raised him.  Immigration officials suspected that the boy was the woman’s nephew, not her son, and therefore not legally entitled to residency.  Based on the DNA fragments, is it possible that she is the boy’s mother?
a)
Yes
b)
No
21.
Bob was found dead in his home.  Crime scene analysis showed signs of a struggle.  Blood was collected from the crime scene, the victim, and three suspects.  Which suspect likely committed the crime? 
a)
Sue
b)
Lisa
c)
John
d)
None of these
22.

What technique allows DNA to be copied outside a living cell?

a)

electrophoresis

b)

PCR

c)

STR

d)

RFLP

23.

DNA fragments can be sorted according to their size by the technique of ___________

a)

PCR

b)

electrophoresis

c)

STR

24.

What does CODIS stand for?

a)

Crazy Old Dogs Intimidate Snakes

b)

Convicted Offender DNA Index System

c)

Combined DNA Index System

d)

Cameron Occasionally Dreams Instead of Studying

25.

What is the special protein that targets a specific base sequence and cuts DNA into smaller fragments?

a)

Gene

b)

DNA Probe

c)

Exon

d)

Restriction Enzyme

26.

If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?

a)

2

b)

4

c)

8

d)

32

27.

On an electropherogram, alleles are represented by...

a)

peaks on a graph

b)

letters like AA or Aa

c)

blue dots on a test strip

d)

band on an autorad

28.

How many STR regions are analyzed for CODIS in the U.S.?

a)

2

b)

10

c)

15

d)

13

29.

Which region of the DNA sequence below is repeated 3 times in the following sequence:


TGCACGATCATCATCATAGCCCT

a)

TCA

b)

TGC

c)

CAT

d)

TAG

30.

On human chromosomes, the locus (location) marked AMEL is a person's:

a)

gender

b)

eye color

c)

age

d)

race

31.

At a particular STR locus on a person's chromosomes, if they have 2 DIFFERENT lengths of the STR repeat, how is it calculated for frequency? The multiplication process is:

a)

2x (1st # of repeats x 2nd # of repeats)

b)

2x (# of repeats)

c)

2x (# of repeats2)

d)

(# of repeats2)

32.

Who is credited for discovering DNA fingerprinting?

a)

Watson & Crick

b)

Edmond Locard

c)

Rosalind Franklin

d)

Alec Jeffreys

33.

The STR segments on your DNA are inherited from your parents. So for each STR segment you have __ alleles.

a)

1

b)

2

c)

3

d)

4

34.

The base pair that starts with "A" is called

a)

Adenine

b)

Alazine

c)

Alpharel

d)

Aquazine

35.

DNA is shaped like what?

a)

Cluster

b)

Double Helix

c)

Single Helix

d)

Ladder

36.

What is the base pair that starts with a "C"?

a)

Cytosol

b)

Cytosine

c)

Colori

d)

Clambus

37.

Which other base pair of DNA does Thymine match with?

a)

Guanine

b)

Adenine

c)

Cytosine

d)

Uracil

38.

What are some examples of common DNA samples left somewhere?

a)

Blood

b)

Hair cells

c)

Skin cells

d)

All of the above

39.

Where is DNA found?

a)

Cell Nucleus

b)

Lysosome

c)

Golgi body

d)

Mitochondria

40.
If base-pairing rules state that Adenine always pairs with Thymine, and Cytosine pairs with Guanine, what would be the complementary strand to ACGGT?
a)
ACGGT
b)
TGGCA
c)
TGCCA
d)
ACCGT
41.
The purpose of PCR
a)
cutting DNA
b)
creating RFLPs
c)
amplifying/copy DNA
d)
reversing the charge of DNA
42.
If base-pairing rules state that Adenine always pairs with Thymine, and Cytosine pairs with Guanine, what would be the complementary strand to ACGGT?
a)
ACGGT
b)
TGGCA
c)
TGCCA
d)
ACCGT
43.
What enzyme is used during PCR to pair complementary bases to the template strand?
a)
Thermonuclease
b)
Helicase
c)
DNA Polymerase
d)
RNA Polymerase
44.
How do scientists cut DNA into smaller strands?
a)
restriction enzymes
b)
scissors
c)
ligase
d)
agarose
45.
Based on these results whose blood was found in the blood stain at the crime scene?
a)
Bob
b)
Sue
c)
John
d)
Lisa
46.
The computerized database used to store DNA information is: 
a)
AFIS
b)
NIBIN
c)
CODIS
d)
PDQ
47.
What is the charge of DNA?
a)
positive
b)
negative
c)
neutral
48.

DNA fragments can be sorted according to their size by the technique of ___________

a)

PCR

b)

electrophoresis

c)

STR

49.

What does CODIS stand for?

a)

Crazy Old Dogs Intimidate Snakes

b)

Convicted Offender DNA Index System

c)

Combined DNA Index System

d)

Cameron Occasionally Dreams Instead of Studying

50.

If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?

a)

2

b)

4

c)

8

d)

32

51.

How many STR regions are analyzed for CODIS in the U.S.?

a)

2

b)

10

c)

15

d)

13

52.

The STR segments on your DNA are inherited from your parents. So for each STR segment you have __ alleles.

a)

1

b)

2

c)

3

d)

4

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