Which of the following best describes competitive binding assays?

Identify the characteristic associated with noncompetitive enzyme immunoassays.

Which of the following best describes homogeneous assays?

In which of the following testing situations is there a direct relationship between the amount of bound label and the amount of patient antigen present?

Compared to a heterogeneous assay, a homogeneous assay:

You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration. Each kit uses a different enzyme and substrate. What is the most important consideration in making your choice?

Which assay format is the best choice for measuring small molecules, such as drugs and small peptide hormones?

Point-of-care testing is performed most easily using:

Compared with a capture assay, a radioimmunoassay is likely to be more advantageous in terms of:

The signal measured in a fluorescence polarization immunoassay is the:

Rheumatoid factor can cause false-positive results in which type of assay?

Extremely high analyte concentrations can cause false-negative results (hook effect) in which type of assay?

Which statement about noncompetitive enzyme immunoassays is true?

Which compound would be an appropriate label in a chemiluminescent assay?

In a capture assay to determine the presence of a viral antigen in a patient sample, which of the following would be on solid phase?

The use of vitamin B7 in a delayed capture assay is found in which technique?

An indirect ELISA assay was performed on a patient's serum sample. What is the relationship of the amount of enzyme activity to the amount of patient antibody measured?

An immunoassay is performed in the following way: Antigen is bound to a solid support. Patient serum is added, and patient antibodies bind to the antigen. The well is washed, and enzyme-labeled anti-immunoglobulin antibodies are added. The well is washed, and enzyme substrate is added. Enzyme activity is determined. Which immunoassay is described?

When is enzyme-labeled antibody added in the sandwich immunoassay testing technique?

The “sandwich” in the sandwich immunoassay testing techniques is between:

To detect low molecular weight analytes, which immunoassay is best?

A urine drug screen is performed using which technique below? (LO 9, T2)

A out-patient physician’s office is investigating the addition of a convenient way to perform analysis for a commonly tested analyte where results are often needed before the physician enters the examination room. Which is most appropriate?

Pregnancy testing can be performed at home using:

Which is more sensitive?

Which immunoassay is sensitive enough to detect very low and very high analyte concentrations?

Indirect immunofluorescence is similar to which type of enzyme immunoassay in terms of the performance of the assay?

An antigen is fixed to a microscope slide, then incubated with patient serum. After washing, fluorescently labeled anti-human immunoglobulin is applied. After an additional wash, the slide is inspected with a fluorescent microscope. This technique is called:

A tissue section is fixed to a microscope slide, then incubated with a fluorescently labeled antibody that binds to an antigen expressed by cells in that tissue. The preparation is then washed and inspected with a fluorescent microscope. This technique is called:

Which immunoassay provides results indirectly proportional to the concentration of patient antigen present?

Therapeutic drug monitoring for phenytoin is commonly tested using which technique? (LO 13, T2)

Which assay format has the lowest equipment cost?

Which assay poses the greatest risk of exposure to biohazards? (LO 14, T1)

Select the technique that would most likely be used to determine the concentration of a therapeutic drug.

The standard method for detecting antinuclear antibodies (in certain autoimmune diseases) is:

Which is a testing interference that would cause a false positive result?

Which is a testing interference that would cause a false negative result?