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WorksheetsLateral Flow Assay Development
Total questions: 10
Worksheet time: 4mins
What is the core principle of protein purification by AKTA system?
Liquid chromatography
Solvent-solvent extraction
Mass spectrophotometry
Recombinant technology
Which of the following is an application example of protein purification?
Extraction of total protein from cell lysate
Gene expression studies
Antigen-receptor interaction studies
Isolation of antibody from serum or plasma
Which of the following technique is not suitable as first step in protein purification?
Size exclusion chromatography
Hydrophobic interaction
Affinity chromatography
Ion-exchange chromatography
Which of the following is the ultimate advantage of using prepacked column on an automated system in protein purification?
Better resolution of separation
Higher protein purity
Higher protein yield
Better reproducibility
What can we use to analyze the purity of fraction after purification?
SDS-PAGE gel with Coomassie stain
Western blot
PCR
Ultrafiltration
What is the first component in lateral flow kit:
Glass/Cellulose Fiber Sample Pad
Glass Fiber Conjugate Pad
Nitrocellulose Membrane
Cellulose Fiber Wicking Pad
What is the key considerations select right sample pad, conjugate pad and absorbent pad
Thickness
Wicking Rate
Water absorption
All of the above
What is the key considerations select right Nitrocellulose membrane?
Capillary rise time
Total caliper (of membrane)
Membrane backing
All of the above
What is the most critical component in a lateral flow assembly
Glass/Cellulose Fiber Sample Pad
Glass Fiber Conjugate Pad
Nitrocellulose Membrane
Cellulose Fiber Wicking Pad
Does Cytiva offer lateral Stater Pack?
Yes
No
