Etched ring in the mouth the pipette:

When making solutions

A solution in which the molecules of solute and solution are in equilibrium with excess undissolved molecules is referred to as:

C-peptide is formed during the conversion of proinsulin to insulin. The amount of circulating C-peptide provides reliable indicator of pancreatic and insulin secretion it is increased in:

CSF glucose concentration is approx. ______ of plasma concentration.

Every 1% increase in HbA1c value causes a change in the plasma glucose by

Advantage of POCT

Which glucose method can employ a colorographic oxygen electrode?

Hyperglycemic hormone released by the adrenal medulla.

TRUE of brittle diabeter (Type 1 DM/IDDM)

I. Ketosis prone diabetes

II. Decreased or undetectable C-peptide

III. Receptor deficient diabetes

IV. Non-insulin dependent diabetes

Which is true regarding insulin.

Short term glucose control

Long term glucose control

Specific and reference method for glucose

Solution of Calcium chloride (CaCl) conatins 3 g per 100 mL.

Which of the ff. is TRUE of glucose

Purpose of immediate spin crossmatch

What do Coomb’s Control cells (check cells) consist of?

Donor: Anti-A = 0 , Anti-B = 0 – (Type O)

Patient: A-cells= 0, B-cells= 0 – (Type AB)

If crossmatched what happens.

Anti-A= 0 Anti-B= 4+

A cells= 0 B cells= 0

An Indirect Antiglobulin Test, DROPS (Major crossmatch), is done. The result was negative. Check cells were added but still no agglutination. What to do?

Which of the ff. will NOT correlate with positive DAT.

2+ gel technology

What does it mean if no agglutination occurs during the AHG phase when adding check cells

Type AB recipient is crossmatched with type O blood.

What incompatibilities are not detected in the Antiglobulin phase of the crossmatch

I. IgM alloantibodies in the recipient serum

II. ABO incompatibilities

III. IgG alloantibodies in the recipient serum

IV. Room temp incompatibilities

Who discovered ABO

Source of anti-B lectin

Blood preservatives provides energy or glucose to RBC. A substrate of ATP.

Blood is considered a drug

Which coincides with ABO blood grouping.

A cells=0

B cells=+

Major Advantage of Gel Column Technology

Purpose of AHG reagent

Blood type A patient

Anti-A=0 Anti-B=+

A cells=0 B cells=0

RBC lifespan

A. TC pipet will dispense the volume indicated

B. TD pipet holds or contains a particular volume, but does not dispense the particular volume

Which of the following are false?

I. Blowout Pipet has a continuous etched ring/two small close, continuous rings located at the top of the pipet

II. Last drop of liquid should be expelled into the receiving vessel when using a self-draining pipet

III. Blowout pipet: User allows content to be drained by gravity

Which is true?

The only pipet which the tip should remain in contact with the side of the vessel for several seconds after the liquid has been drained

Pipet for biological fluids with viscosity greater than water

Pipet with greatest degree of accuracy. Should be used when diluting standards

Designed to transfer a fixed amount/single volume of liquid

A. TC pipet dispensed indicated volume

B. TD pipet holds a particular volume but does not dispense particular volume.

Blowout pipet = Drain contents of pipet by gravity

Aspiration angle

Dispensing angle

Transfer pipets, except:

a. Volumetric

b. Pasteur

c. Ostwald-Folin

A Proper ratio of plasma to red cells is important for accuracy in antigen-antibody reaction

Preferred specimen for Red Cell Suspension:

A. Hemolysis of RBCs can cause false results

B. Too heavy or too light cell suspension can cause false positive/negative

Cells are washed using:

a. distilled water

b. isotonic saline

c. 0.9 NaCL

Fill the tube ___full using ___when preparing RBC suspension

Centrifuge minutes for RBC suspension

In preparing RBC suspension, you are required to wash completely for 3-5 times with NSS to remove traces of plasma

A procedure to test compatibility of donor red cells and patient serum [DROPS]

Also known as the incubation at 38°C is Thermophase

Agglutination at high protein phase

Clinically significant antibodies are detected at what phase

Clinically significant antibodies:

Amount of patient serum added for crossmatching

Other term for Immediate spin phase

Most important blood group for transfusion practice

Correct frequency of ABO blood groups:

ABO Antibodies include:

Anti-A = 4+

Anti-B= 2+

A cells= 0

B cells= 4+

Positive autocontrol in antibody screening

Grading of crossmatching:

several large agglutinates, clear background

Crossmatching: Type B patient and Type O donor.

Which one will be incompatible?

Using known sources of anti-sera to detect antigen

Most frequent test performed in the blood bank:

A. individuals normally produce antibodies against antigens absent from their RBCs

B. All other defined blood groups do not regularly have naturally occurring antibodies to antigens lacking from their RBCs

Reagents used in reverse typing, except:

A. Human blood

B. 3-5% RBC suspension

What is the most serious, potentially life-threatening reaction?

Which does not require the transfusion to stop and investigation to be performed?

Hemolytic transfusion reaction

Component that carries the highest risk of bacterial contamination

Most common transmitted viral infection

SG of copper sulfate solution

Minimum Hb for autologous donors

Improves the survival of RBCs

prevents caramelization

substrate for ATP

maintains pH during storage

Maximum allowable storage time for RBCs defind by recovery of ___% RBCs in___hours

RBC solution that acts as stabilizing agent

Decreased during RBC storage, except:

Proper procedure for preparation of platelets from whole blood

Granulocyte: life-threatening systemic infections not controlled by antibiotics

Blood processing must occur within

Minimum hemoglobin for female donor

Blood donor requirements except:

a. Identification card

b. 2 Tubes = used for screening

c. Donor information

Permanent deferral except:

Amount of anticoagulant in the blood bag

Blood should be processed within

For TA-GVHD, but not febrile transfusion reaction