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AST & Mechanisms Quiz

Total questions: 22

Worksheet time: 23mins

Name
Class
Date
1.

A standardized inoculum of bacteria (usually 0.5Mcf) is dabbed onto the surface of a dish of Mueller-Hinton (MH) agar. Filter paper discs impregnated with antimicrobial agents are placed on the agar. After overnight incubation, the diameter of the zone of inhibition is measured around each disc.

a)

1st Statement is true.

b)

2nd Statement is true.

c)

Both Statements are true.

d)

Both Statements are false.

2.

To determine whether the bacterial isolate is capable of expressing resistance to the antimicrobial agents selected for treatment is the _____________ of AST.

a)

Primary Purpose

b)

Primary Goal

c)

Principle

d)

Primary Objective

3.

The purpose of AST is to guide the clinician in selecting appropriate causative agent. To gather epidemiological data on microbial resistance.

a)

First Statement is true.

b)

Second Statement is true.

c)

Both Statements are true.

d)

Both Statements are false.

4.

All of the following are the reason/s for standardization of AST, except:

a)

To optimize bacterial growth conditions

b)

To optimize conditions for maintaining antimicrobial integrity and activity

c)

To maintain reproducibility and consistency in the resistance profile

d)

None of the choices

5.

DISK DIFFUSION Method is also known as (a)   .

6.

Medium used in Disk Diffusion Method.

(a)  

7.

Composition of 0.5 McFarland Standard.

(a)  

8.

Standard inoculum of AST.

(a)  

9.

pH of the medium for AST.

(a)  

10.

Depth of the agar for AST.

(a)  

11.

All of the following are factors affecting disk diffusion, except:

a)

Thickness of the agar

b)

Amount of inoculum

c)

pH of the media

d)

None of the choices

12.

The distance between 12 discs.

(a)  

13.

All of the following are condition/s that can cause false sensitive results of AST, except:

a)

Thick medium

b)

Decrease organisms

c)

Increase drying

d)

None of the choices

14.

This term refers to "no zone or small zone of inhibition".

(a)  

15.

Provide microorganisms with the capacity to avoid host defenses and damage host cells, tissues and organs in a number of ways.

(a)  

16.

Match the following:

a)

Glycocalyx

1.

Streptococcus mutans

b)

M protein

2.

Streptococcus pyogenes

c)

Opa protein

3.

Streptococcus pyogenes

d)

Tapered end

4.

Treponema pallidum

17.

Match the following

a)

Coagulase

1.

Coagulate blood

b)

Kinase

2.

Digest fibrin clots

c)

Hyaluronidase

3.

Hydrolyses hyaluronic acid

d)

Collagenase

4.

Hydrolyses hyaluronic acid

18.

Match the following:

a)

Toxigenicity

1.

ability to produce toxins

b)

Toxemia

2.

presence of toxin in host’s blood

c)

Antitoxin

3.

antibodies against a specific toxin

19.

They are part of the outer portion of the cell wall of gram negative bacteria. They are liberated when the bacteria die and the cell wall breaks apart.

(a)  

20.

This term refers "development of disease".

(a)  

21.

This refers to microbes that are present for various periods then disappear.

(a)  

22.

This refers to "pathogens limited to a small area of the body".

(a)