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Enterobacteriaceae

Total questions: 25

Worksheet time: 6hrs 15mins

Name
Class
Date
1.

The ortho-nitrophenyl-β-galactopyranoside (ONPG) test is most useful when differentiating:

a)

A. Salmonella spp. from Pseudomonas spp.

b)

B. Shigella spp. from some strains of Escherichia coli

c)

C. Klebsiella spp. from Enterobacter spp.

d)

D. Proteus vulgaris from Salmonella spp.

2.

At which pH does the methyl red (MR) test become positive?

a)

A. 7.0

b)

B. 6.5

c)

C. 6.0

d)

D. 4.5

3.

In the test for urease production, ammonia reacts to form which product?

a)

A. Ammonium citrate

b)

B. Ammonium carbonate

c)

C. Ammonium oxalate

d)

D. Ammonium nitrate

4.

Decarboxylation of the amino acids lysine, ornithine, and arginine results in the formation of:

a)

A. Ammonia

b)

B. Urea

c)

C. CO2

d)

D. Amines

5.

Putrescine is an alkaline amine product of which bacterial enzyme?

a)

A. Arginine decarboxylase

b)

B. Phenylalanine deaminase

c)

C. Ornithine decarboxylase

d)

D. Lysine decarboxylase

6.

Kligler iron agar (KIA) differs from triple-sugar iron agar (TSI) in the:

a)

A. Ratio of lactose to glucose

b)

B. Ability to detect H2S production

c)

C. Use of sucrose in the medium

d)

D. Color reaction denoting production of acid

7.

Which genera of the Enterobacteriaceae are known to cause diarrhea and are considered enteric pathogens?

a)

A. Enterobacter, Klebsiella, Providencia, and Proteus

b)

B. Escherichia, Salmonella, Shigella, and Yersinia

c)

C. Pseudomonas, Moraxella, Acinetobacter, and Aeromonas

d)

D. Enterobacter, Citrobacter, and Morganella

8.

Care must be taken when identifying biochemical isolates of Shigella because serological cross reactions occur with:

a)

A. E. coli

b)

B. Salmonella spp.

c)

C. Pseudomonas spp.

d)

D. Proteus spp.

9.

Which of the following tests best differentiates Shigella species from E. coli?

a)

A. Hydrogen sulfide, VP, citrate, and urease

b)

B. Lactose, indole, ONPG, and motility

c)

C. Hydrogen sulfide, MR, citrate, and urease

d)

D. Gas, citrate, and VP

10.

Fever, abdominal cramping, watery stools, and fluid and electrolyte loss preceded by bloody stools 2–3 days before is characteristic of shigellosis but may also result from infection with:

a)

A. Campylobacter spp.

b)

B. Salmonella spp.

c)

C. Proteus spp.

d)

D. Yersinia spp.

11.

Which group of tests, along with colonial morphology on primary media, aids most in the rapid identification of the Enterobacteriaceae?

a)

A. MR and VP, urease, and blood agar plate

b)

B. Phenylalanine deaminase, urease, and CDC agar plate

c)

C. Bacitracin, β-lactamase, and MacConkey agar plate

d)

D. Indole, oxidase, MacConkey, and blood agar plates

12.

Which group of tests best identifies the Morganella and Proteus genera?

a)

A. Motility, urease, and phenylalanine deaminase

b)

B. Malonate, glucose fermentation, and deoxyribonuclease (DNase)

c)

C. Indole, oxidase, MR, and VP

d)

D. Indole, citrate, and urease

13.

Enterobacter sakazakii can best be differentiated from Enterobacter cloacae by which of the following characteristics?

a)

following characteristics? A. Yellow pigmentation and negative sorbitol fermentation

b)

B. Pink pigmentation and positive arginine dihydrolase

c)

C. Yellow pigmentation and positive urease

d)

D. H2S production on TSI

14.

Which of the following organisms is often confused with the Salmonella species biochemically and on plated media?

a)

A. E. coli

b)

B. Citrobacter freundii

c)

C. Enterobacter cloacae

d)

D. Shigella dysenteriae

15.

Which single test best separates Klebsiella oxytoca from K. pneumoniae?

a)

A. Urease

b)

B. Sucrose

c)

C. Citrate

d)

D. Indole

16.

Why might it be necessary for both pink (lactose-positive) and colorless (lactose-negative) colonies from an initial stool culture on MacConkey agar to be subcultured and tested further for possible pathogens?

a)

A. Most Shigella strains are lactose positive

b)

B. Most Salmonella strains are maltose negative

c)

C. Most Proteus spp. are lactose negative

d)

D. Pathogenic E. coli can be lactose positive or lactose negative

17.

Which organism is sometimes mistaken for Salmonella and will agglutinate in Salmonella polyvalent antiserum?

a)

A. C. freundii strains

b)

B. Proteus mirabilis strains

c)

C. S. sonnei strains

d)

D. E. coli

18.

Which of the following organisms are generally positive for β-galactosidase?

a)

A. Salmonella spp.

b)

B. Shigella spp.

c)

C. Proteus spp.

d)

D. E. coli

19.

The drugs of choice for treatment of infections with Enterobacteriaceae are:A. Aminoglycosides, trimethoprim–sulfamethoxazole, third-generation cephalosporins

a)

A. Aminoglycosides, trimethoprim–sulfamethoxazole, third-generation cephalosporins

b)

B. Ampicillin and nalidixic acid

c)

C. Streptomycin and isoniazid

d)

D. Chloramphenicol, ampicillin, and colistin

20.

Infections caused by Yersinia pestis are rare in the United States. Those cases that do occur are most frequently located in which region?

a)

A. New Mexico, Arizona, and California

b)

B. Alaska, Oregon, and Utah

c)

C. North and South Carolina and Virginia

d)

D. Ohio, Michigan, and Indiana

21.

Four blood cultures were taken over a 24-hour period from a 20-year-old woman with severe diarrhea. The cultures grew motile (room temperature), gram-negative rods. A urine specimen obtained by catheterization also showed gram-negative rods, 100,000 col/mL. Given the following results, which is the most likely organism?

a)

A. Proteus vulgaris

b)

B. Salmonella typhi

c)

C. Yersinia enterocolitica

d)

D. E. coli

22.

A leg-wound culture from a hospitalized 70-year-old diabetic man grew motile, lactose-negative colonies on MacConkey agar. Given the following biochemical reactions at 24 hours, what is the most probable organism?

a)

A. Proteus vulgaris

b)

B. Serratia marcescens

c)

C. Proteus mirabilis

d)

D. Enterobacter cloacae

23.

Three consecutive stool cultures from a 25-year-old male patient produced scant normal fecal flora on MacConkey and Hektoen agars. However, colonies on CIN agar (cefsulodin–irgasan–novobiocin) displayed “bulls-eye” colonies after 48 hours incubation. The patient had been suffering from enterocolitis with fever, diarrhea, and abdominal pain for 2 days. What is the most likely identification of this gram-negative rod?

a)

A. E. coli

b)

B. Proteus mirabilis

c)

C. Yersinia enterocolitica

d)

D. Klebsiella pneumoniae

24.

Following a 2-week camping trip to the Southwest (US), a 65-year-old male patient was hospitalized with a high fever and an inflammatory swelling of the axilla and groin lymph nodes. Several blood cultures were obtained, resulting in growth of gram-negative rods resembling “closed safety pins.” The organism grew on MacConkey’s agar showing non–lactose-fermenting colonies. Testing demonstrated a nonmotile rod that was biochemically inert. What is the most likely identification?

a)

A. Yersinia pestis

b)

B. Klebsiella pneumoniae

c)

C. Proteus vulgaris

d)

D. Morganella morganii

25.

Direct spread of pneumonic plague disease occurs by which route?

a)

A. Fecal–oral route

b)

B. Rat bite

c)

C. Ingestion of contaminated tissue

d)

D. Inhalation of contaminated airborne droplets