The DNA molecules are immobilized on the _____________ in southern blotting technique.

When was the original method of southern blotting developed?

Which of the following is used as targets in the blotting techniques?

To remove negatively charged molecules through matrix of agarose, nucleic acid molecules are separated by applying

Another name for complementary DNA is:

Commonly used cloning vector include

Cloning vectors are __ DNA molecules in which DNA fragments/insert are maintained and amplified

An ORI is recognized by

Fluorescence of GFP colonies can be visualized at what range?

GFP is isolated from which organism

What colour is GFP when it fluoresces?

The optimal incubation temperature for T4 DNA ligase

What is the size of GFP protein

Which of the following A260/280 ratios would be a reasonable cut off for RNA that you intend to use for quantitative RT-PCR?

Which of these dyes could you use to visualise DNA run on an agarose gel?

Analysis of a sample of DNA found that 20% of the bases were adenine.       

What percentage of the bases would be pyrimidines?

Cuvette For Spectrophotometer

The A260/A280 ratio is influenced considerably by pH of water it is dissolved and the result can vary.

Proteins are usually the major contaminants in nucleic acid extracts and these have absorption Max. at _______nm