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WorksheetsAdvance Molecular Genetics - Module 8
Total questions: 17
Worksheet time: 6mins
The different confirmations of plasmids that exist are...
Linear
Supercoiled
Open circular
Double helix
Alkaline lysis is done by...
NaOH & Triton-X
SDS
Sodium Dodecyl Sulfate and Sodium Hydroxide
Potasium acetate and SDS
What precipitates the DNA?
Isopropanol
Chaotropic Salt
Lysis Buffer
Ethanol
What is the function of neutralization solution?
Denature plasmid DNA only
Denature plasmid and genomic DNA
Precipitates protein
Decrease pH
If you're task with preparing 2 ml of glycerol stock with 50% sterile glycerol solution, how much glycerol stock is needed (in μL) for a final concentration of 15%?
(a)
Which of the following plasmid conformation migrate the fastest in agarose gel electrophoresis?
Open circular
Supercoiled
Linear
Nicked
Which of the following is the correct steps for plasmid isolation?
Lysis - Bind - Neutralize - Wash - Elute
Lysis - Neutralize - Bind - Wash - Elute
Lysis - Neutralize - Wash - Bind - Wash - Elute
Lysis - Wash - Neutralize - Bind - Wash - Elute
What neutralizes alkaline lysed sample?
Ammonium Chloride
HCl
Kalium Acetate
any acid will do
What is the number of fragments predicted to be produced from digesting pGEM-SOD with EcoRI?
1
2
3
4
What precipitates protein during the plasmid isolation?
Neutralization buffer
Binding buffer
Wash Buffer
Alkaline Lysis Buffer
Which of the following are palindromic sequence?
GATATC
GTAATG
ATCGAT
AATTAA
If spectrophotometric analysis of purified plasmid sample resulted in A260/280 value of 1.3, what can we say about the sample?
Contaminated with Phenol
High purity
Broken spectrophotometer
Contaminated with protein
Contaminated with RNA
Spectrophotometric analysis of purified plasmid sample resulted in the A260 value of 1.2. Given that A260 of 1.0 equal to 50µg/ml pure dsDNA, how much plasmid is in 10 μL of sample? (give the answer in ng)
(a)
Both CRISPR-Cas9 and Restriction enzyme could produce blunt-end cut on DNA
True
False
What is the predicted DNA fragment length produced from pGEM-SOD digestion using EcoRI?
2997 bp and 170 bp
3000 bp and 190 bp
2997 bp only
2997 bp and 190 bp
Which of the following plasmid conformation that correspond to its appropriate ladder size when visualized in agarose gel electrophoresis?
single-stranded circular
Open circular
Linear
Supercoiled
How is the test question?
Easy peasy lemon squeazy
Knees weak, arms heavy, mom's spaghetti
Just right
Boring
Fascinating
