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Ch3 Microbial growth

Total questions: 79

Worksheet time: 44mins

Name
Class
Date
1.

When bacteria are inoculated into a

liquid growth medium........?

a)

we can plot of the number of cells in

the population over time.

b)

we can not plot of the number of cells in

the population over time.

c)

Period of adjustment to new condition.

d)

Little or no cell division occurs, population size doesn’t increase.

2.

which the correct answer about Decline phase:

a)

Cell division being to slow down .

b)

Cell number decrease at a logarithmic rate.

c)

Overall cell number doesn't increase.

d)

All of them

3.

which the plate that colonies will be on surface?

a)

Pour plate

b)

Spread plate

4.

which the plate that appear under agar surface?

a)

Pour plate

b)

Spread plate

5.

Used mainly to measure bacteria that will not grow on solid medium:

a)

Filtration

b)

Plate count

c)

MPN

d)

Statistical method

6.

what the disadvantage of plate count:

a)

Must perform serial dilutions to get appropriate numbers/plate.

b)

Cannot always distinguish between live and dead bacteria.

c)

Motile bacteria are difficult to count.

d)

Requires a high concentration of bacteria (10 million/ml).

7.

the population size begins to stabilize in phase?

a)

Lag phase

b)

Log phase

c)

Stationary phase

d)

Decline phase

8.

Which the following phases of bacteria growth?

a)

Catalase phase

b)

Peroxide phase

c)

Microaerophils

d)

Lag phase

9.

Which the correct answer about the pour plate?

a)

Introduce a 1.0 or 0.1 ml inoculum into an empty Petri dish.

b)

Add liquid nutrient medium kept at 50oC.

c)

Gently mix, allow to solidify, and incubate

d)

Requires a high concentration of bacteria (10 million/ml).

10.

Cannot always distinguish between live and dead bacteria is disadvantags of :

a)

Filtration

b)

Plate count

c)

MPN

d)

Direct microscopic count

11.

which the disadvantages of turbidity:

a)

Requires a high concentration of bacteria (10 million/ml).

b)

Cannot always distinguish between live and dead bacteria.

c)

Requires a high concentration of bacteria (10 to 100 million cells/ml)

d)

Not useful for heat sensitive organisms.

12.

Which the no incubation time required?

a)

Direct microscopic count

b)

Filtration

c)

Turbidity

d)

Dry weight

13.

which the Not correct answer about the Turbidity:

a)

As bacteria multiply in media, it becomes turbid.

b)

Stain is added to visualize bacteria.

c)

Use a spectrophotometer to determine % transmission or absorbance.

d)

Multiply by a factor to determine concentration.

14.

which the correct answer about the log phase?

a)

May last from one hour to several days.

b)

Phase of intense metabolic activity, in which individual organisms grow in size.

c)

Period of most rapid growth.

d)

All of them.

15.

Most frequently used method of measuring bacterial populations.

a)

Plate count

b)

Filtration

c)

MPN

16.

Used to measure small quantities of

bacteria.

a)

Plate count

b)

Filtration

c)

Most probable number

d)

Direct microscopic count

17.

which the correct answer about the direct microscopic count:

a)

A specific volume of a bacterial suspension (0.01 ml) is placed on a microscope slide with a special grid.

b)

Stain is added to visualize bacteria.

c)

Cells are counted and multiplied by a factor to obtain concentration.

d)

All of them

18.

in metabolic activity, As bacteria multiply in media, they produce certain products:

a)

Carbon dioxide

b)

Vitamin

c)

Sulfur

d)

Acids

19.

Microbial growth refers to an increase in …

a)

Cell size

b)

Cell number

c)

Cell weight

d)

Chromosomes

20.

Bacteria grow and divide in a …

a)

slow and simple process

b)

rapid and complex process

c)

rapid and simple process

d)

Slow and complicated process

21.

which of the following is not correct regarding true psychrophiles:

a)

Optimum growth 15°c or below

b)

Sensitive to temperature over 20°c

c)

Usually cause disease or food spoilage

d)

Are cold loving

22.

psychrotrophs are responsible for most low temperature food spoilage:

a)

TRUE

b)

FALSE

23.

most bacteria and pathogens and common spoilage organisms are:

a)

Mesophiles

b)

Thermophiles

c)

Psychrophiles

d)

Psychrortophs

24.

mesophiles are adapted to live in plant cells

a)

TRUE

b)

FALSE

25.

Which of the following is not correct regarding thermophiles:

a)

Many cannot grow below 45°C

b)

Optimum growth at 80°C

c)

Some thermophiles form extremely heat resistant endospores

d)

Non of the above

26.

most bacteria prefer:

a)

Neutral pH level

b)

Medium heat

c)

Alkaline pH level

d)

A and B

27.

acidic environment ease microbial growth

a)

TRUE

b)

FALSE

28.

alkalinity used for food preservation

a)

TRUE

b)

FALSE

29.

Which of the following used to stabilize pH:

a)

Catalase

b)

Peroxidase

c)

Buffers

d)

Plasmolysis

30.

acidophiles grow at:

a)

(0.1-6)pH

b)

(2.4-9)pH

c)

(0.1-5.4)pH

d)

(7-12)pH

31.

chemohetrotrophs obtain carbon from:

a)

Lipids

b)

Carbohydrates

c)

Proteins

d)

All of the above

32.

extreme halophiles require very high salt concentration

a)

TRUE

b)

FALSE

33.

bacteria in the dead sea are:

a)

Facultative halophiles

b)

Extreme halophiles

c)

Halophiles

d)

Thermophiles

34.

carbon is the backbone of al inorganic compounds

a)

TRUE

b)

FALSE

35.

chemoautotrophs and photoautotrophic obtain carbon from:

a)

Proteins

b)

Lipids

c)

Carbon dioxide

d)

All of the above

36.

halophiles require …… salt concentration

a)

Low

b)

High

c)

Moderate to large

d)

Very high

37.

hypotonic solution lets the water..

a)

Exit from the cell

b)

Enter to the cell

c)

No effect on the cell

38.

neutrophphiles include most pathogens

a)

TRUE

b)

FALSE

39.

Hypertonic solution removes …. From the cell

a)

mRNA

b)

Fats

c)

Water

d)

Ribosomes

40.

cells are 80-90% Carbon

a)

TRUE

b)

FALSE

41.

alkaliphiles grow at (7-12)pH

a)

TRUE

b)

FALSE

42.

which the correct answer about the log phase?

a)

May last from one hour to several days.

b)

Period of most rapid growth.

c)

Phase of intense metabolic activity, in which individual organisms grow in size.

d)

All of them

43.

which of the following is not of the sources of nitrogen

a)

sulfates

b)

Nitrates

c)

Ammonium

d)

protein

e)

Nitrogen gas

44.

A large sample (100 ml or more) is filtered to:

a)

Killed bacteria

b)

Retain bacteria

c)

Rearrangement bacteria

d)

Non of them

45.

…….. used to form thiamin and biotin

a)

Sulfur

b)

Phosphate

c)

Nitrogen

d)

Sulfates

46.

sulfur is used to form

a)

potassium and magnesium

b)

Protein and vitamin

c)

Sulfate and phosphorus

d)

DNA and RNA

47.

sulfates salts that dissociate to give NO2

a)

❌

b)

✅

48.

Which the Doesn’t distinguish live and dead cells:

a)

Dry weight.

b)

Metabolic activity.

c)

Turbidity

49.

in dry weight

a)

Bacteria or fungi in liquid media are centrifuged.

b)

Resulting cell pellet is weighed.

c)

Measure metabolic products.

50.

Determines 95% probability that a bacterial population falls within a certain range:

a)

Filtration

b)

Plate count

c)

MPN

d)

Statistical method

51.

Nitrates salts that dissociate to give?

a)

SO4

b)

NO3

c)

N2

d)

NAO2

52.

Factors that slow down microbial growth ( toxic waste, Acidic pH, limited nutrients) example of?

a)

Lag phase

b)

Log phase

c)

Stationary phase

d)

Decline phase

53.

It’s used to form amino acid, DNA and RNA

a)

sulfide

b)

Phosphorus

c)

Sulfur

d)

Nitrogen

54.

……used to form DNA,RNA,ATP and phospholipids.

a)

Phosphorus

b)

Hydrogen sulfide

c)

Sulfate

d)

protein

55.

………. is required for cell wall synthesis in Gram positive bacteria

a)

Copper

b)

magnesium

c)

Calcium

d)

Potassium

56.

……… use to produce  more energy from nutrients than anaerobes

a)

Iron

b)

copper

c)

zinc

d)

Oxygen

57.

which of the following is true about Obligate Aerobes?(يوجد اكثر من اجابة صحيحه)

a)

Cannot use oxygen

b)

is harmed by the presence of toxic of oxygen

c)

Oxygen dissolves poorly in water

d)

Require oxygen to live

58.

E.coli , Staphylococcus and yeasts are Example of:

a)

Aerotolerant Anaerobes

b)

Obligate Anaerobes

c)

Obligate Aerobes

d)

Facultative Anaerobes

59.

Clostridium bacteria that cause tetanus and botulism, Example of:

a)

Aerotolerant Anaerobes

b)

Obligate Aerobes

c)

Obligate Anaerobes

d)

Facultative Anaerobes

60.

Pseudomonas, common nosocomial pathogen, Example of:

a)

Aerotolerant Anaerobes

b)

Obligate Anaerobes

c)

Obligate Aerobes

d)

Facultative Anaerobes

61.

………..Can not use oxygen, but tolerate its presence.

a)

Aerotolerant Anaerobes

b)

Obligate Aerobes

c)

Obligate Anaerobes

d)

Facultative Anaerobes

62.

…………Require oxygen, but at low concentrations. (Sensitive to toxic forms of oxygen.)

a)

Superoxide

b)

Facultative Anaerobes

c)

Microaerophiles

d)

Singlet Oxygen

63.

Campylobacter is Example of:

a)

Superoxide

b)

Facultative Anaerobes

c)

Microaerophiles

d)

Singlet Oxygen

64.

Nitrogen fixing bacteria use for:

a)

produce oxygen

b)

produce oxygen

c)

fertilise soil naturally

65.

which of the following is true about (singlet oxygen)

a)

present in Facultative Anaerobes

b)

present in Anaerobes

c)

present in superoxide

d)

present in phagocytic cells

66.

singlet oxygen are extremely toxic and reactive form of oxygen

a)

❌

b)

✅

67.

All organisms growing in

atmospheric oxygen must produce an enzyme:

a)

Catalase

b)

superoxide dismutase

c)

microaerophiles

d)

anaerobes

68.

SOD is made by aerobes, anaerobes and facultative anaerobes

a)

✅

b)

❌

69.

There are two different enzymes that break down hydrogen peroxide.(يوجد اكثر من اجابة)

a)

acetate

b)

benzoyl

c)

peroxidase

d)

Catalase

70.

……….Breaks hydrogen peroxide into water and O2.

a)

acetate

b)

benzoyl

c)

peroxidase

d)

Catalase

71.

………..Converts hydrogen peroxide into water

a)

peroxidase

b)

benzoyl

c)

Catalase

d)

acetate

72.

benzoyl peroxide is Example of:

a)

superoxide dismutase

b)

catalase

c)

Hydrogen Peroxide

d)

singlet oxygen

73.

where the bacteria can growth?

a)

Culture Medium

b)

Air

c)

In liver

d)

At surface

74.

……….Microbes that grow and multiply in or on a culture medium.

a)

Nutrient

b)

culture

c)

Culture medium

d)

Medium

75.

Bacterial Division occurs mainly by:

a)

generation fission

b)

Binary fission

c)

Three fission

d)

one fission

76.

A few bacterial species reproduce by budding

a)

❌

b)

✅

77.

Time required for a cell to

divide, and its population to double

a)

Generation time

b)

Division time

c)

Disease time

d)

Bleeding time

78.

which of the following is False about generation time

a)

Some bacteria require over 24 hours to divide.

b)

some bacteria will die

c)

Most bacteria divide every 1 to 3 hours

d)

E. coli divides every 20 minutes

79.

Legumes cultivation is used to fertilize soil naturally

a)

❌

b)

✅

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