WorksheetsImmunotherapy
Total questions: 85
Worksheet time: 1hrs 25mins
Form of biotherapy that uses certain parts of the immune system to fight diseases.
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Collection of antibodies which can be isolated from donated blood provided by other individual.
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Plays an important role in the first line of defense against viral infection.
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Added to many vaccines to increase immunogenicity and efficacy.
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Derived from blood of animals or individuals who have been exposed to a given antigen.
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An example of antisera that is derived from an animal presumably a horse which has been injected with snake venom.
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Get antibodies from the spleen of the mouse and then add to the human cancerous myeloma cell.
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Part mouse and part human
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Fully human
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10x much smaller than mabs and penetrate into tissues more easily. Much more effective and less time consuming.
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Very widely used monoclonal antibody technique. Detecting the formation of an immunocomplex, the linkage of antibodies and antigens.
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Used to identify parts of a cell in basic research and in diagnosing a test.
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Uses laser detectors and computer technology to find the antigen associated in the cell.
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Binding agent that is used to isolate specific molecules from solution.
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Used to purify or to remove the antigen from the antibody.
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Auto Greek word for self. Abnormal functioning of the immune system that causes your immune system to produce antibodies against your own tissues.
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Lupus, rheumatoid arthritis, systematic sclerosis
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Multiple sclerosis and myasthenia gravis.
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insulin dependent / juvenile diabetes
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inflammatory bowel disease
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The immune system is attacking the normal cells and destroys them.
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Autoimmune diseases resulting from antibody stimulating receptors on certain cells.
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An example of auto immune disease under neuromuscular. Latin and greek in origin, literally means “serious muscle weakness”.
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Slow or suppress the immune system responses in an attempt to stop the inflammation involve in the autoimmune attack.
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Involve in a wide range of physiologic systems such as stress response, immune response and regulation of inflammation.
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Decreases the number of circulating lymphocytes.
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Used to treat certain cancer and rheumatoid arthritis.
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A class of diseases in which a cell, or a group of cells display uncontrolled growth.
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Vaccine mixed with strep and staph bacteria which produces Erysipelas which causes the malignant tumor to disappear.
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was considered as the Father of Immunotherapy
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Use to treat bladder cancer (BCG)
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Interact with cells in order to regulate the body’s response and to trigger disease and infections.
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Use to treat kidney cancer
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Use to stimulate the body’s immune system to fight some types of cancer.
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These are cytotoxic weapons that seek and kill only the cancer cells.
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used against breast cancer
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chronic use of drug, alcohol, or similar substances
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Major obstacles in transplantation. Incompatibility with the transplanted organ. The organs are called allograft.
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The production and emission of light
by a living organism.
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Type of medical test that identifies
changes in genes, chromosomes, or proteins.
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A method of identifying genetic material.
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Any of a class of natural or synthetic substances
composed of very large molecules.
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used to signify an enzyme
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Proteins that help speed up
metabolism, or the chemical reactions in
our bodies.
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(a) of a DNA fragment to millions and billions copy of the genetic material.
Pair of primers hybridizes with the sample DNA and defines
the region that will be amplified, resulting in millions and millions of
copies in a very short timeframe.
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THE DOUBLE-STRANDED TEMPLATE DNA IS
HEATED BY 94 TO 95 DEGREE CELCIUS TO SEPARATE IT INTO TWO
SINGLE STRANDS.
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THE TEMPERATURE IS LOWERED BY 50 TO 65 DEGREE
CELCIUS TO ENABLE THE DNA PRIMERS TO ATTACH TO THE
TEMPLATE DNA.
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THE TEMPERATURE IS RAISED NY 72 DEGREE CELCIUS
AND THE NEW STRAND OF DNA IS MADE BY THE TAQ POLYMERASE
ENZYME.
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It automates the repetitive step of
amplifying specific DNA sequences.
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Application of Polymerase Chain Reaction
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Used when you are investigating a gene and have no
easy way to determine if the gene is being expressed.
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Types of reporter genes
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Fluoresce based reporter
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Enzyme based colorimetric reporter.
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Bioluminescence based reporter.
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Cells-comes from a Greek word
“Soma” meaning body.
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cells forming the body of
organisms and are known as diploids
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Biological cells that give rise to
the formation of gametes(sperm and egg
cell) and are known as haploids.
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Highly specialized
cells and are fully developed.
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Primitive and do
not perform high level function and they
have no specialized functions.
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Undifferentiated cells that can
be specialized. They have the ability to
reproduce indefinitely.
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Derived from adult tissues,
from umbilical cords and fetal tissues.
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Derived from the
early stage of embryo.
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Cells from which
all other cells with specialized functions are
generated.
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Types of stem cells
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mostly used to produce cattle
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What is the first successful
cloned animal
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Decreed that the creation of a human embryonic stem cell line is a felony.
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Are single-celled organisms,
commonly used as host cells for making copies
of DNA in the lab because they are easy to
grow in large numbers. Their cellular
machinery naturally carries out DNA
replication and protein synthesis.
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is a DNA-joining enzyme
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Any whole food whose genetic
material has been altered using genetic
engineering techniques.
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Joining two DNA fragments via
a ligase-catalyzed reaction.
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A small circular DNA molecule
found in bacteria and some other
microscopic organisms.
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The
term used to describe the combination of
two DNA strands that are constructed
artificially.
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A protein produced
by bacteria that cleaves DNA at specific
sites along the molecule.
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The process by which
exogenous DNA is transferred into the
host cell.
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The use of high-
voltage electric shocks to introduce
DNA into cells.
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The bacterial cells are incubated with
recombinant DNA on ice, followed by placing them
briefly at 42o C and then putting it back on ice.
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A DNA molecule (often plasmid or virus) that is
used as a vehicle to carry a particular DNA segment into a
host cell as part of recombinant DNA technique.
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Extra-chromosomal, self-replicating,
double-stranded circular DNA molecules found in
various bacteria and in some yeast.
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Viruses that infect bacterial
cells by injecting their DNA into these cells.
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Fusion of DNA molecules
from two distinct species.
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STEPS OF GENETIC
RECOMBINATION TECHNOLOGY
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IMPORTANCE OF RECOMBINANT
DNA TECHNOLOGY
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