WorksheetsBiotech Quiz Review
Total questions: 29
Worksheet time: 21mins
This laboratory procedure is known as
CLONING
gel electrophoresis
chromatography
use of a dichotomous key
A student performed a gel electrophoresis experiment. The results are represented in the diagram
below. Compared to the fragments at the top of the gel, the fragments at the lower end are
larger, and move slower
larger, and move faster
smaller, and move faster
smaller, and move slower
This technique used to analyze DNA involves the
synthesis of new DNA strands from subunits
separation of DNA fragments based on size
production of genetically engineered DNA molecules
removal of defective genes from DNA
Does PCR require a primer?
Yes
No
What is the denaturation step?
mutating RNA polymerase
Cooling DNA to allow primers to form
separating DNA helix in a cold environment
separating DNA helix with using heat
The laboratory process that makes many copies of DNA
PCR
Electrophoresis
Gene Therapy
What is the denaturation step?
mutating RNA polymerase
Cooling DNA to allow primers to form
separating DNA helix in a cold environment
separating DNA helix with using heat
What happens after you have denatured your DNA?
It denatures again.
Primers attach to the gene of interest.
The gene elongates
PCR is over
What PCR "ingredient" would be different in these two tests?
CRISPR can edit DNA. What organisms naturally has CRISPR?
Viruses
Humans
Plants
Bacteria
Why do bacteria have CRISPR? Check all that apply.
To be able to remember which viruses they have been infected with
To be able to edit virus DNA
To cut apart virus DNA thereby stopping the virus infection
To be able to edit their own DNA
What is the role of the guide RNA?
It helps the cell repair the DNA
ensures that the Cas9 enzyme cuts at the right point in the genome
Recognizes PAM sequences and opens up the DNA
makes a cut across both strands of the DNA
How does the guide RNA help the cell know where to cut the DNA?
Guide RNA helps to find the PAM
Guide RNA is complementary to the DNA sequence
Guide RNA always binds to the same sequence of DNA
Like restriction sites, they are always palindromes
What does the cas9 protein do?
Prevents viruses from entering bacteria
Cut RNA
Cut DNA
Binds to RNA
How does CRISPR edit DNA? Check ALL that apply.
CRISPR chemically changes A's to C's
CRISPR cuts the DNA and the cell makes errors when it tries to fix the broken DNA
CRISPR cuts the DNA and the cell can use a similar DNA sequence as a template to try to fix the DNA
CRISPR changes all PAM's so that they are no longer able to be recognized
CRISPR-Cas9 is a type of ________ system found in bacteria.
digestive
endocrine
circulatory
immune
