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WorksheetsQuiz Molecular Biology
Total questions: 20
Worksheet time: 10mins
The alternative selectable marker _______________ and chromogenic substrate __________________ that selects recombinants on the basis of their ability to produce colour
LacZ gene ; X-gal
IPTG ; β-galactosidase
Amp Resistance gene ; X-gal
β-galactosidase ; IPTG
__________ is also known as "light" chromatin and represents DNA that can actively undergoing __________.
Heterochromatin, translation
Euchromatin, translation
Heterochromatin, transcription
Euchromatin, transcription
This notable figure is the one that discover DNA component in the pus of wounded soldier’s bandages
Thomas Morgan
Friedrich Miescher
Phoebus Levene
Thomas Avery
Name the histone protein that used to link between nucleosomes
H2A, H2B
H3
H4
H1
Identify the statement that is not true regarding restriction enzymes used in recombinant DNA technology.
They are site-specific
They recognize palindromic sequences
They are endonucleases
They are ATP dependent nucleases
Identify the false fact about nucleases
Nucleases catalyze the hydrolytic cleavage of the phosphodiester linkages.
Nucleases can be exonuclease and endonucleases.
Exonucleases cleave the sequence by staggered cutting
Exonucleases cleave the chain randomly between any nucleotides.
Non-essential genes/stuffer fragments are removed from bacteriophage because
To reduce the phage genomic RNA size
To increase the phage genomic DNA size
To fit in other phage DNA
To fit in the foreign gene to be cloned
Cosmid is a hybrid between _________ and _____________ because ______________ .
phage, plasmid, they are packaged into a λ phage head but they replicate as plasmid in bacteria
cosmid, plasmid, they behave like phage by infecting bacteria but replicate in phage head
phage, plasmid, they behave like phage by infecting bacteria but replicate in phage head
bacteria, plasmid, they are packaged into a λ phage head but they replicate as plasmid in bacteria
What is the capacity of cosmid vector?
10-12 kb
20 kb
45-50 kb
300kb
Statement below is true, EXCEPT:
Mendel’s shows the genes can exist in several different forms, or alleles.
Morgan provided the first definitive evidence for the chromosome theory using Drosophila melanogaster as experimental subject.
Watson and Crick proposed the x-ray diffraction data to map the DNA models based on chemical and physical data.
McClintock shown a physical demonstration of recombination in maize chromosomes.
Function of the restriction enzymes were primarily as:
Defence mechanism of protozoa against antibiotics
Tool by virus to cleave bacterial genome
Defence mechanism of bacteria against bacteriophages viruses
Detection of recognition site by phage
How do you overcome vector religation problem?
Use ligase enzyme
Use alkaline phosphatase enzyme
Use restriction enzymes
Use polymerase enzyme
Chromosomes are maximally coiled at
Prophase
Metaphase
Anaphase
Telophase
The histone proteins present in a histone octamer are
H2A,H2B,H2C,H3
H2A,H2B,H1,H3
H2A,H2B,H3,H4
H1A, H1B, H2, H3
What is a palindrome?
A sticky end resulting from a restriction enzyme
A blunt end resulting from a restriction enzyme
A sequence that reads the same forwards and backwards
A sequence that is complementary to the other
Identify the selectable marker for recombinant plasmid
LacZ gene
ORI
Multiple cloning sites
Antibiotic resistance gene
In order to insert a human gene into plasmid, both must
code for the same gene product.
be cut by the same restriction enzyme.
originate from the same type of cell.
have identical sequences.
The chromogenic substrate __________________ that able to produce blue colour with the presence of __________.
X-gal, IPTG
X-gal, β-galactosidase
LacZ gene, X-gal
LacZ gene, β-galactosidase
The colonies of recombinant bacteria appear white because of
insertional inactivation of β-galactosidase gene in recombinant bacteria
insertional inactivation of α-galactosidase gene in non-recombinant bacteria
Inactivation of glycosidase gene in recombinant bacteria
Incompetence of recombinant bacteria containing β-galactosidase
The host cell of E.coli DH5alpha is made chemically competent by
giving them a freeze shock
giving them a heat shock
giving them electrical current
giving them chemical treatment
