WorksheetsMMI 1061 Midterm Review (Fall 2023)
Total questions: 52
Worksheet time: 27mins
What is the shape and arrangement of this organism?
Rods in chains
Single rods
Rods in pairs
Rods in clusters
What is the shape and arrangement of this organism?
Tetrad
Chains of cocci
Clusters of cocci
Bacillus in pairs
What is this part of the microscope called?
Fine Focus Knob
X-Y Stage Control
Condenser Centering Screw
Coarse Focus Knob
What is this part of the microscope called?
Objectives
Field Diaphragm
Condenser
Lamp
What is this part of the microscope called?
Coarse Focus Knob
Condenser Diaphragm
Stage Adjustment Knob
Fine Focus Knob
What is this part of the microscope called?
Field Diaphragm
Condenser Diaphragm
Lamp
Slide Holder
What is this part of the microscope called?
Eyepieces
Stage Adjustment Knob
Condenser Centering Screw
Revolving Nosepiece
Which of the following are the correct way to handwrite scientific names?
Italicized name
Underline both genus and species
Genus is capitalized
Species is uppercased
What is it called when a culture contains only one species of microorganisms?
Sterile Culture
Liquid Culture
Pure Culture
Mixed Culture
What is it called when a stock culture contains two or more species of microorganisms?
Sterile Culture
Mixed Culture
Complex Culture
Pure Culture
What type of growth pattern observed in this picture?
Sediment
Flocculent
Pellicle
Uniform Fine Turbidity
What growth pattern is observed in this picture?
Pellicle
Sediment
Flocculent
Uniform Fine Turbidity
What growth pattern is observed in this picture?
Sediment
Uniform Fine Turbidity
Pellicle
Flocculent
In this image, which of these following media are defined media?
Brain-Heart Infusion Broth
Glucose Salts Broth
Nutrient Broth
Which of the following media supports the growth of fastidious organisms?
Brain-Heart-Infusion Broth
Glucose Salts Broth
Nutrient Broth
In this Fluid Thioglycollate Broth experiment, what type of anaerobe/aerobe is Pseudomonas aeruginosa?
Aerotolerant anaerobe
Obligate Anaerobe
Obligate Aerobe
Microaerophile
In this Fluid Thioglycollate Broth experiment, what type of anaerobe/aerobe is Staphylococcus aureus?
Obligate anaerobe
Microaerophile
Facultative anaerobe
Aerotolerant anaerobe
What type of hemolysis is observed on this blood agar plate?
Beta or β - hemolysis
Alpha or α - hemolysis
Gamma or γ - hemolysis
What type of hemolysis is observed on this blood agar plate?
Beta or β - hemolysis
Alpha or α - hemolysis
Gamma or γ - hemolysis
What type of hemolysis is observed on this blood agar plate?
Beta or β - hemolysis
Gamma or γ - hemolysis
Alpha or α - hemolysis
What is the shape and arrangement of the bacteria being observed in this image?
Rods in single
Cocci in single
Rods in chains
Cocci in clusters
What is the shape and arrangement of this bacteria in this image?
Cocci in tetrads
Bacillus in pairs
Cocci in clusters
Bacillus in clusters
In this gram staining of E. coli and S. aureus, which of these bacteria are gram-negative?
Escherichia coli
Staphylococcus aureus
True or False: Basic stains (i.e Crystal Violet) bind to the cell wall of bacteria because the cell walls are positively charged.
True
False
True or False: Zig-zag plate streaking is a good method to isolate high density cultures.
True
False
True or False: Gram stain is a staining technique that allows for the differentiation of bacteria cells based on the structural differences in their cell wall.
False
True
What conclusion would you make for this Phenol Red Fermentation Test?
Carbohydrate was fermented with acid production but no gas
Carbohydrate was not fermented
Carbohydrate was fermented with acid and gas production
Peptones were degraded
What conclusion would you make for this Phenol Red Fermentation Test?
Carbohydrate was not fermented
Peptones were degraded
Pink Broth
Red Broth
What conclusion would you make for this Phenol Red Fermentation Test?
Carbohydrate was not fermented
The broth is red
The broth is orange
Carbohydrate was fermented with acid production but no gas
What is the enzyme that is present in a positive Nitrate Reduction test?
Nitrite reductase
Multiple enzymes
Nitrase
Nitrate reductase
A culture tube of Alcaligenes faecalis has undergone a Nitrate Reduction Test. What conclusion would be made here?
Nitrate was reduced to Nitrite (NO3 → NO2);
Nitrate reductase is present (+)
Denitrification occurred (NO3 → NO2 → NO, ½N2O or ½N2) (+)
No nitrate reduction occurred (-)
What conclusion would you make observing this gelatin hydrolysis test if the control is solid?
Gelatin is hydrolyzed; gelatinase is present
Gelatin is hydrolyzed; gelatinase is not present
Gelatin is not hydrolyzed, gelatinase is not present
Gelatin is not hydrolyzed, gelatinase is present
What reagents are used to determine Simple Nitrate Reduction?
Alpha-naphthylamine, sulfanilic acid & zinc dust
Alpha-naphthol & sulfanilic acid
Alpha-naphthylamine & sulfanilic acid
Alpha-naphthol, sulfanilic acid & zinc dust
True or False: The microscopes that we used in our lab are Compound Light Microscopes that utilize Bright-field microscopy.
True
False
What conclusion can be made when observing the P. mira tube for the Urea Hydrolysis test?
Urea is hydrolyzed; Urease is produced
Urea is not hydrolyzed; Urease is not produced
Urea is hydrolyzed; Urease is not produced
Urea is not hydrolyzed; Urease is produced
Which of these following characteristics does not qualify to be a part of the Enterobacteriaceae family?
Gram-negative bacillus
Simple nitrate reducers
Lactose fermenter
Citrate Positive
What does the "M" in the IMViC test series stand for?
Methylene Blue
Methyl Red
Methyl Orange
Methyl Violet
In the Methyl Red experiment, Methyl Red dye, a pH indicator, is used when observing Mixed Acid Fermentation. What is the end product of interest?
Acetoin
Strong stable bases
Ammonia (NH3)
Strong stable acids
What is the substrate for the Citrate Utilization Test?
Citrate permease
Ferric ammonium citrate
Sodium thiosulfate
Sodium citrate
In the Citrate Utilization Test, if there is growth but the tube is green, is it positive or negative?
Positive
Negative
What is the substrate for the MRVP test?
Mannitol
Glucose
Lactose
Galactose
What does it mean when there is a red layer on top of the broth in the Vogues Proskauer test?
Mixed acid fermentation occurred
2,3 butanediol fermentation occurred
Mixed acid fermentation did not occurred
2,3 butanediol fermentation did not occur
True or False: The MR and VP test both uses the Mixed Acid Fermentation pathway as their biochemical process.
True
False
True or False: The organism in this SIM medium reduces sulfur, produces indole and is motile.
True
False
The SIM medium is a semi-solid medium. In this SIM test, is the organism in question motile?
Yes
No
What objective lens is used when setting up for Koehler Illumination on a compound light microscope?
4X
10X
40X
100X
Which objective lens requires the use of immersion oil?
4X
10X
40X
100X
Where should gloves be discarded after use?
In the small biohazard bin
In the trash can
In the large biohazard bin
In the sink
True or False: The technique used when working with microorganisms without contamination is called "aseptic technique".
True
False
What do you use to disinfect the lab work space before working?
Wescodyne
Windex
Water
Where do broken glass and used microscope slides are disposed in?
Small biohazard bin
Large biohazard bin
Sharps container
Trash can
What is the temperature in celcius is the incubator set at? (Just type the number)
(a)
