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Worksheets

DNA Tools 12 & 11 G

Total questions: 17

Worksheet time: 13mins

Name
Class
Date
1.

In a laboratory experiment, Evelyn, Jackson, and Isla are studying restriction enzymes. They learn that restriction enzymes recognize and bind to specific DNA sequences and cleave the DNA within that sequence.

a)

True

b)

False

2.

There are 4 types of Nitrogen bases in DNA : A U G C

a)

True

b)

False

3.
This cuts DNA at specific sequences
a)
Plasmid
b)
Recombinant DNA
c)
Restriction enzyme
d)
Gel electrophoresis
4.
This diagram represents which of the following? 
a)
Gel electrophoresis
b)
Gene therapy
c)
Cloning 
d)
Genetic engineering
5.
The manipulation of DNA is called
a)
Genetic engineering
b)
Karyotype
c)
Plasmid
d)
Gel electrophoresis
6.

How do restriction enzymes "know" where to cut DNA

a)

A specific sequence of nucleotides

b)

They cut randomly

c)

Distance from the centromere

7.

"Sticky-ends" are DNA strands with:

a)

single-stranded ends

b)

ends cut flat by an enzyme

c)

no way to be recombined

8.
Which is used to cut DNA molecules at specific points?
a)
gel electrophoresis
b)
PCR analysis
c)
a restriction enzyme
d)
recombinant DNA
9.
Which 2 molecules form the sides (backbone) of the DNA ladder?
a)
deoxyribose sugar and adenine
b)
deoxyribose sugar and a hydrogen bond
c)
deoxyribose sugar and the nucleus
d)
deoxyribose sugar and phosphate
10.

What is the correct term for a restriction enzyme?

a)

DNA ligase

b)

Taq polymerase

c)

Endonuclease

d)

Manorlakease

11.

What type of cut is this photo?

a)

Blunt

b)

sticky

12.

is the total DNA present in the nucleus of each cell

a)

genome

b)

restriction enzymes

c)

EcoR1 cut

13.

proteins that recognize and bind to specific DNA sequences and cleave the DNA within that sequence

a)

genome

b)

restriction enzymes

c)

EcoR1 cut

14.

the enzymes have been discovered in bacteria?

a)

viruses

b)

portions

c)

bacteria

15.

which of the following sequences the EcoR1 cut

a)

GAATTC

b)

CCCATG

c)

GGATCC

16.

Organism that contains genes from two different organisms

a)

gel electrophoresis

b)

cell transformation

c)

transgenic organisms

d)

restriction enzyme

17.

Process where DNA fragments can be separated and analyzed by placing it in a gel and applying an electric current

a)

gel electrophoresis

b)

polymerase chain reactions

c)

recombinant DNA (rDNA)

d)

clone