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WorksheetsDNA RECOMBINANT
Total questions: 40
Worksheet time: 30mins
What is the name of insulin produced by genetic engineering?
Humulin G
Humulin I
Humulin H
Humulin S
What is a cDNA?
collection of total genomic DNA
non-coding RNA molecule (containing about 22 nucleotides) found in plants,
complementary DNA molecules synthesized from mRNA
small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules
What is the purpose of amplification?
To screen for recombinant bacteria.
To join the rDNA with the host cell.
To produce multiple copies of the desired gene.
To prevent the bacteria from reproducing.
Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?
amplification
insertion
transformation
screening
The cell that able to receive recombinant DNA for cloning purpose known as
vector
target DNA
plasmid
host cell
Which of the following is NOT a tool used in genetic engineering?
plasmid
restriction enzyme
DNA helicase
DNA ligase
Which restriction enzyme listed below was isolated from E. coli bacteria?
EcoRI
HindIII
HaeII
SmaI
Which of the following is an enzymatic protein that join DNA fragment between gene of interest and plasmid?
plasmid
restriction enzyme
ligase
protein expression
Restriction enzymes....
Cut DNA at a specific site
Can be used to cleave gene of interest
Can be used to cut vector DNA
Do all of these things
A struture of plasmid that contain gene of interest are called?
nonrecombinant plasmid
recombinant DNA
binary
selective species
Cell that receive recombinant DNA for cloning purpose
Host cell
plasmid
DNA target
Gene of interest
To produce desired protein _____________ should be taken from any source like human, animal and plant.
recombinant DNA
Target DNA
plasmid
ligase
Which of the following are TRUE of restriction enzymes?
I. It restricts transcriptionI.
II. It is found in all eukaryotic cells.
III. It acts on palindromic sequences.
IV. It is sensitive to changes in temperature and pH.
I and II
I and III
II and IV
III and IV
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells.
IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V) Use ligase to seal plasmid DNA to non-plasmid DNA
In the screening process, clones that metabolize X-gal turn_____________.
yellow
white
red
blue
Which of the following is CORRECT about host cell?
Plasmid is an example of host cell.
Unable to receive DNA molecule through transformation.
An organism that prevents cloning vectors from multiplying.
An organism that is used to receive recombinant DNA for cloning purposes.
What is the purpose of amplification?
To screen for recombinant bacteria.
To join the rDNA with the host cell.
To produce multiple copies of the desired gene.
To prevent the bacteria from reproducing.
Which of these pairs is incorrectly matched?
DNA ligase – mapping human chromosomes
Target DNA – gene of interest
Taq polymerase – PCR
Plasmid as a vector – isolated from bacteria
In screening process, colonies that were white in the presence of X-gal contained:
Plasmid DNA only
Foreign DNA only
Both foreign DNA and plasmid
Neither plasmid nor foreign DNA
What happens in the Denature step of PCR?
The DNA nucleotides are broken apart.
The base-pairing rules for DNA are reversed.
The double-stranded DNA is separated into two single strands of DNA.
From where does Taq polymerase come?
Bacteria that live in hydrothermal vents/hot springs.
Cambodia
The rain forest
At what temperature does the denaturation step of PCR occur?
95° C
50° C
72° C
At what temperature does the Extension step of PCR occur?
95°C
50-65°C
72°C
What is a cDNA?
collection of total genomic DNA
non-coding RNA molecule (containing about 22 nucleotides) found in plants,
complementary DNA molecules synthesized from mRNA
small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules
double stranded cDNA is produced by ……………………….
Ligase enzyme
Reverse Transcriptase enzyme
Restriction Endonuclease enzyme
DNA polymerase enzyme
Multiple cloning site (MCS) contains
Promoter region
Antibiotic resistance gene
Ori
Restriction sites
Arrange in a correct seq
insertion, cut, screening, transfomation, isolation, screening
Isolation, insertion, cut, transformation, amplification, screening
insertion, transformation, amplification, screening
Isolation, cut, insertion, transformation, amplification, screening
which one is the sequence that EcoRI will recognize
CCCGGG
GGATCC
GAATTC
GGGCCC
