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Madi’s Wednesday labs

Total questions: 7

Worksheet time: 6mins

Name
Class
Date
1.

The independent variable(s) in our experiment is​/are ​

a)

soil not from auto shop

b)

soil from auto shop

c)

soil contaminated with motor oil

d)
microbial biodiversity
2.

The dependent variable(s) in our experiment is/are​ ​ ​

a)
soil pH
b)
moisture content
c)
microbial diversity
d)
your mom
e)
soil texture
3.

What is the purpose of the Ecoplate?

a)

to see what carbon sources are utilized in our soil sample

b)

to test for microbial biodiversity

c)

to reinforce our pipetting and teamwork

d)

to teach us about richness, evenness, and Shannon Index

4.

What is the purpose of 16S rRNA sequencing?

a)

to determine the diversity of the microbiome in our soil samples

b)

to determine whether straw is helping the plants grow

c)

to determine what bacteria are in our soil samples

d)

to determine if a certain bacterium can survive in our experimental condition

5.

Why is 16s rRNA important?

a)

It isn’t double stranded

b)

It is the most variable region in a bacteria’s DNA

c)

16 is my lucky number

d)

Dr. Adler said it was

6.

DOUBLE POINTS!!!! Why would soil scientists and the urban gardening community care about our experiment?

Categorize the following

improve crop yield

improve soil quality

microbial biodiversity

applicable to other studies

Urban Gardening
Soil Scientists
7.

DOUBLE POINTS!!! The greatest dog ever according to Madi is

(a)