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DNA replication

Total questions: 22

Worksheet time: 8mins

Name
Class
Date
1.

Which of the following describes the difference between a prokaryote mRNA and eukaryote mRNA?

a)

Prokaryotes have longer mRNA strands due to presence of introns

b)

Eukaryotes have longer mRNA strands due to the presence of introns

c)

Prokaryotes modify the mRNA due to the presence of a nuclear envelope

d)

Eukaryotes modify the mRNA due to the absence of a nuclear envelope

2.

Which of the following is the function of the poly-A tail?

a)

To protect mRNA from hydrolytic enzymes

b)

To allow for exit from nucleus

c)

To signal for termination of translation

d)

To decrease the length of mRNA molecules

3.

In the absence of which factor or enzyme, the transcription starts non-specifically (hypothetically) at any base of DNA ?

a)

Rho ( ρ ) factor

b)

Core enzyme

c)

Sigma ( σ ) factor

d)

None of the above

4.

Which of the following sequences of RNA will be transcribed from a hypothetical sequence of DNA given below?

3'-ATGCATGCATGCATGC-5'

5'-TACGTACGTACGTACG-3'

a)

3'-UACGUACGUACGUACG-5'

b)

5'-AUGCAUGCAUGCAUGC-3'

c)

5'-UACGUACGUACGUACG-3'

d)

3'-AUGCAUGCAUGCAUGC-3'

5.

Which of the following is a wrong match?

a)

Rho factor - Terminates translation

b)

Template strand – Has same sequence as of mRNA

c)

Sigma factor - Initiates translation

d)

All of these

6.

Name the enzyme that facilitates opening of DNA helix during transcription.

a)

DNA polymerase

b)

RNA polymerase

c)

DNA ligase

d)

DNA helicase

7.

RNA must be edited before it can be used to make Proteins, what happens during this editing process?

a)

A spliceosome cuts out exons, reconnects the introns

b)

A spliceosome cuts out the introns, reconnects the exons

c)

A splicesome pairs nucleotides with the DNA base pairs

d)

The RNA is checked for typos

8.

What enzyme does transcription use?

a)

DNA polymerase

b)

ligase

c)

RNA polymerase

d)

helicase

9.

RNA polymerase reads the mRNA strand in which direction?

a)

3' to 5' direction

b)

5' to 3' direction

10.

What type of RNA moves to the ribosome to start protein synthesis?

a)

tRNA

b)

mRNA

c)

rRNA

d)

nRNA

11.
what does mRNA do?
a)
carry info from genes to ribosomes
b)
carry amino acids to ribosomes
c)
help with RNA splicing
d)
make up ribosomes
12.
Which is a difference between DNA and RNA
a)
RNA is double stranded
b)
RNA is not made of nucleotides
c)
RNA contains Uracil instead of Thymine
d)
RNA does not contain a sugar
13.

Many enzymes are involved in DNA replication. Which is the correct order of enzymes involved in DNA replication from beginning to end?

a)

Helicase, DNA primase, DNA polymerase, Ligase

b)

RNA primase, Helicase, DNA polymerase, Ligase

c)

Helicase, RNA primase, DNA polymerase, Ligase

d)

DNA primase, Helicase, RNA polymerase, Ligase

14.

Which shows the correct complementary DNA strand of ATG?

a)

ATG

b)

TAC

c)

TAG

d)

CGC

15.
Based on the diagram, the enzyme (represented in gray) is most likely...
a)

DNA polymerase

b)

Ligase

c)

Primase

d)

Helicase

16.
Based on the diagram, how many origins of replication are represented?
a)
1
b)
2
c)
3
d)
4
17.

Which letter in the picture below depicts the leading strand in DNA replication?

a)

A

b)

C

c)

B

d)

None of the above

18.

Which is the second step?

a)

DNA Helicase unwinds original DNA strand

b)

RNA Primase lays down RNA primer for new nucleotide bases.

c)

DNA polymerase builds new strands by bring in new nucleotide bases.

d)

Ligase knits together fragments on lagging strand

19.

The picture below shows an enzyme unwinding and unzipping DNA.

​

​What is the name of this enzyme?

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase

d)

RNA Polymerase

20.

What is needed for DNA replication to unzip at the hydrogen bonds?

a)

phosphates

b)

enzymes

c)

deoxyribose (sugar)

d)

hydrogen

21.

The process of DNA replication is called semi conservative because it results in

a)

none of these

b)

Two DNA molecules.  One original molecule and one with two new strands

c)

two exact copies with new strands

d)

two DNA molecules, each with 1 original strand and one new.

22.

In what phase of the cell cycle does DNA replication take place?

a)

G1

b)

S

c)

G2

d)

Prophase