Free Printable Bacteria Culturing and Morphology

Worksheets

Bacteria Culturing and Morphology

Total questions: 35

Worksheet time: 28mins

Name

Class

Date

Thick peptidoglycan in cell wall

Gram positive bacteria

Gram negative bacteria

Thin peptidoglycan in cell wall

Gram positive bacteria

Gram negative bacteria

Stained pink

Gram positive bacteria

Gram negative bacteria

Stains purple

Gram positive bacteria

Gram negative bacteria

This is an example of the staining of what type of bacteria?

That's not bacteria

Gram positive

Both types of bacteria

Gram negative

The main difference between GRAM positive and GRAM negative bacteria is?

They have no difference

Ribosomes and nucleoid

Its cell wall

Its scourges

Which part of the bacteria contains Peptidoglycan?

Lipoproteins

Cell wall

Cell membrane

Porin channel

Which type of bacteria contains more than 1 layer of phospholipids?

Gram Negative

Gram Positive

Both (-) and (+)

Which layer of the bacteria is dyed/stained purple?

Cell membrane

cell wall

capsule

slime layer

10.

These bacteria are

gram positive

gram negative

nonliving

viral

11.

These bacteria are

gram positive and negative

gram positive

gram negative

viral

12.

coccus

bacillus

spirilla

none of these

13.

Name the three cell shapes of prokaryotes

rod, circle spiral

cocci, bacilli, spirilli

crystal, tube, spaghetti

14.

The purpose of streaking a plate is to obtain

large quantities of bacteria

bacteria in pure culture

15.

When streaking a plate, the loop should be flamed

At the beginning and end

At the beginning and end and between the first 2 zones

At the beginning and end and between the second and third zone

At the beginning and end and between each zone

16.

What type of bacteria is this?

Coccus

Bacillus

Spirillum

Virus

17.

What is agar

growth medium in which microorganisms can be grown

nutrient broth in which microorganisms can be grown

growth medium in which protists can be grown

nutrient broth in which viruses can be grown

18.

When streaking a plate, the loop should be flamed

At the beginning and end

At the beginning and end and between the first 2 zones

At the beginning and end and between the second and third zone

At the beginning and end and between each zone

19.

When streaking a plate, the lid should be

placed on the bench top, top side up

placed on the bench top, inside surface up

should be held in the same hand as the plate

should be held in the opposite hand from the plate

20.

Flaming the neck of the test tube after uncapping the tube and before recapping is essential during culture transfer.

True

False

21.

What is the inoculation loop used for?

Nutrients for bacteria

Sterilising equipment

Collecting/spreading bacteria

Stopping contamination

22.

What is the bunsen burner used for?

Nutrients for bacteria

Sterilising equipment

Collecting/spreading bacteria

Stopping contamination

23.

What type of environment is best for the growth of most microorganisms?

Hot and dry

Sunny, cold and dry

Warm, dark and moist

Cold and wet

24.

If you see someone in a laboratory using aseptic technique, what might that person be doing?

Talking to a co-worker

Looking through a microscope

Properly cleaning equipment

Adjusting the room temperature

25.

What is the goal of aseptic techniques

To prevent contamination of the worker only

To prevent contamination of the environment only

To prevent contamination of the worker and the environment

26.

How can we avoid the agar plate being cross contaminated with unwanted microorganisms when it is open? Pick all that apply?

Work near a bunsen

Wipe the surface of the agar with antiseptic

Minimise the time the lid is open and close it as soon as you are finished inoculation

Pass the agar through the bunsen flame

27.

Which of the following correctly represents the aseptic technique?

When you no longer need the petri dish, it can be left open

all equipment must be sterilized before culturing

Clean work space upon entering-not leaving; that is the next person's job

hand washing is not a part of aseptic technique - just universal precautions

28.

The goal of streak-plating is

to obtain pure colonies

to see what all grows

to count the total number of colonies

to diagnose disease

29.

After using an inoculating loop or needle to transfer a culture from one medium to the next, what stop should be taken next to avoid contamination.

Heat the inoculating loop or needle with the bunsen burner flame until it becomes cool to the touch.

Heat the inoculating loop or needle with the bunsen burner flame until it becomes purple hot.

Heat the inoculating loop or needle with the bunsen burner flame until it becomes red hot.

30.

When should lab bench tops be cleaned with disinfectant solution?

Never, take a chance!

At the beginning and end of lab.

Only at the end.

31.

How should agar plates be incubated

Right side up

Upside down

Sideways

All of the above

32.

What is the first thing you should do?

put on gloves

wash your hands

sterilize your workspace

33.

What is the last thing you should do?

take off your gloves

wash your hands

sterilize your workspace

34.

Which of the following instruments are used to perform a streak plate.

Incinerating loop

Inoculating loop

Clamp

Stock photo

35.

George streaks a plate of medium from a broth culture. After incubation, he sees confluent growth in all sectors. Which of the following errors could NOT explain this result?

He forgot to pick a colony from the plate

He forgot to flame the loop between sectors

He picked additional culture from the plate after the first sector

He crossed over the previous streak too many times at the beginning of a new sector.

Reset

Bacteria Culturing and Morphology

Total questions: 35

Worksheet time: 28mins

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