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Biotech Review

Total questions: 39

Worksheet time: 33mins

Name
Class
Date
1.

Gel electrophoresis is a laboratory technique designed to separate molecules such as DNA, RNA, or proteins based upon what property?

a)
Size and charge
b)

the number of nucleic acids

c)

the number of amino acids

d)
Color and shape
2.

What similarity between a blood sample collected at the site of a robbery and that of a suspect would indicate that the suspect was most likely responsible?

a)
matching hair color
b)

matching DNA bands

c)
same blood type
d)
similar fingerprints
3.

Once the strands of DNA are cut in various sizes, which explanation best describes why some strands of DNA travel further in the gel than others?

a)
Charge of the DNA fragments
b)
Temperature of the gel
c)
Concentration of the DNA fragments
d)
Size of the DNA fragments
4.

What types of enzymes are used to cut strands of DNA into pieces before placing the DNA in a gel for gel electrophoresis?

a)

Strands of DNA have different charges, and the negative strands travel further than positive strands.

b)

restriction enzymes

c)

digestion enzymes

d)

Strands of DNA have different charges, and the positive strands travel further than negative strands.

e)

Mr. Cruthirds

5.

What is a practical application for the process illustrated?

a)

Translation and Transcription

b)

DNA Fingerprinting

c)

Gene Therapy

d)

Human Genome Project

6.

In preparation for an electrophoresis procedure, enzymes are added to DNA in order to

a)

convert the DNA into gel

b)

cut the DNA into fragments

c)

change the color of the DNA

d)

produce longer sections of DNA

7.
Based on these results whose blood was found in the blood stain at the crime scene?
a)
Bob
b)
Sue
c)
John
d)
Lisa
8.
Which suspect is likely guilty?
a)
Suspect #1
b)
Suspect #2
c)
Suspect #3
9.
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
a)
DNA is negatively charged so attracted to the positive end of the unit
b)
DNA is positively charged to attracted to the negative end of the unit
c)
the agarose gel in negatively charged
d)
the agarose gel is positively charged
10.
In gel electrophoresis, the largest DNA fragment will appear
a)
closest to the starting wells
b)
farthest from the starting wells
c)
three quarters away from the starting wells
d)
it depends on how many fragments there are
11.
DNA is recovered from a suspected dead soldier.  Which Parents are the likely parents of the soldier?
a)
Parents C and D
b)
Parents E and F
c)
Parents A and B
d)
Parents G and H
12.

What is the purpose of PCR?

a)

To produce millions of copies of the DNA genome

b)

To produce millions of copies of a specific region of DNA

c)

To add nucleotides to a DNA sequence

d)

To watch polymerase work.

13.

What happens in the denature step of PCR?

a)

The DNA nucleotides are broken apart.

b)

The base-pairing rules for DNA are reversed.

c)

The double-stranded DNA is separated into two single strands of DNA.

d)

The DNA is returned to its natural setting.

14.

What is the function of a primer?

a)

To identify the particular region of DNA to be copied by PCR.

b)

To copy DNA.

c)

To create DNA nucleotides.

d)

To maintain the temperature of the PCR reaction.

15.

If the target sequence is GCATTA, what is the complementary sequence?

a)

CGUAAU

b)

CGTAAT

c)

CGTUUT

d)

GCATTA

16.
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
a)
It allows the strands of DNA to be cut into various lengths for testing
b)
It restricts the number of base pairs that can be tested in a sample
c)
It makes the testing simpler by moving the strands into the gel faster
d)
It charges the DNA strands
17.

How many restriction sites appear in the picture?

a)

1

b)

2

c)

3

d)

4

18.

What kind of cuts were made in the scenario above?

a)

Blunt

b)

Sticky

c)

No cuts were made

d)

Palindrome

19.

How many bp make up each fragment from left to right?

a)

3 bp, 5 bp, 4 bp

b)

4 bp, 5 bp, 3 bp

c)

7 bp, 13 bp, 8 bp

d)

8 bp, 13 bp, 7 bp

e)

5 bp, 9 bp, 5 bp

20.

What is genetic transformation?

a)

A prokaryotic cell turning into a eukaryotic cell

b)

Foreign DNA being inserted into a plasmid

c)

A host organism taking in foreign DNA

d)

A gene being mutated

21.

What is the circle of DNA inside of a bacteria called?

a)

plasmid

b)

desired gene

c)

recombinant DNA

d)

insulin

22.

How is the plasmid cut open?

a)

scissors

b)

restriction enzymes

c)

catalase enzyems

d)

human genes

23.

Insulin producing genes from a human could be an example of the

a)

plasmid

b)

restriction enzyme

c)

bacteria cell

d)

desired gene

24.

The plasmid with new DNA being inserted into the bacteria is called

a)

recombinant DNA

b)

desired gene

c)

plasmid

d)

free floating DNA

25.

A bacteria with human genes could be called a

a)

clone

b)

eukaryote

c)

transgenic organism

d)

stem cell

26.

A bacteria with a human-insulin gene could

a)

be ingested as medicine

b)

produce insulin medicine

c)

causes deadly diseases

d)

cure diabetes

27.

Which is not a GMO?

a)

transgenic bacteria

b)

corn with pesticide produce genes

c)

cloned sheep

d)

tomatoes with genes to last longer

28.

Restriction enzymes

a)

cut open DNA to be modified

b)

piece together DNA sections

c)

help digest proteins

d)

help assemble proteins

29.

What is the name of the process in which new breeds and varieties of organisms are created using artificial selection?

a)

Artificial selection

b)

Natural selection

c)

Selective breeding

d)

Survival of the fittest

30.

What is the name of the process in which new breeds and varieties of organisms are created using artificial selection?

a)

Artificial selection

b)

Natural selection

c)

Selective breeding

d)

Survival of the fittest

31.

In which ways can genetic engineering can improve crops

a)

Make them pest or drought resistant

b)

Make them more nutritious

c)

Make them larger

d)

All answers are correct

32.
Why aren't more scientists using gene modification?
a)
Because they are poor
b)
Because they are lazy
c)
Because of ethics and morality
d)
Because of how long it takes
33.
It is very common for yellow labs to have bad hips. This is because owners want purebred yellow labs who are good hunting dogs.  This is an example of 
a)
Natural Selection
b)
Selective breeding
c)
Mitosis
d)
Fossil Record
34.
Scientists genetically engineer corn to be immune to corn diseases. This is BENEFICIAL because
a)
This causes corn to be less likely to reproduce
b)
This causes corn to be less likely to survive
c)
This causes corn to be smaller
d)
This allows increased production of corn
35.

Which process is involved with genetic engineering?

a)

Allowing organisms to reproduce without human interference

b)

Humans choosing individuals with desirable traits to parent the next generation

c)

Using technology to cut DNA and insert genes to get the traits you want.

36.

Inserting a gene for bioluminescence (glowing) into fish is an example of...

a)

Genetic engineering

b)

Natural selection

c)

Artificial selection

d)

Selective breeding

37.

A positive reason(s) to genetically modify (GMOs) foods include...

a)

Produce larger fruits

b)

Make pest resistant vegetables

c)

Produce foods that grow faster

d)

All of these are positives of GMOs

38.

A concern(s) about genetically modified foods (GMOs) include..

a)

Risk to human health

b)

Loosing the original genetic line of the food source

c)

May be harmful to ecosystems

d)

All of these are valid concerns

39.

Humans with genetic diseases can have a gene inserted into their body using a virus. This is called:

a)

Gene therapy

b)

Cloning

c)

DNA fingerprinting

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