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biology pracitcal exam review

Total questions: 38

Worksheet time: 38mins

Name
Class
Date
1.

-Calibrates the Spectrophotometer

-prevents outside variable to be measured

-allows only the desired molecules to be read

(a)  

2.

  • color: yellow (light coloration)

  • Measure amount of reducing sugar (Maltose)

  • Redish-dark amber is the opposite color when no maltose is identified



(a)  

3.

  • color: clear/transparent (opposite is black-dark blue)

  • Measure the amount of starch there is

  • Interacts with and binds to starch



(a)  

4.

Unit 4 independent variable

(Hint: spectrometer with serial dilution)

(a)  

5.

Unit 4 dependent variable

(Hint: spectrophotometer with serial dilution)

(a)  

6.

What reagent is this?

  • Color: blue

  • Measure the amount of protein concentration

  • Higher protein concentration the deeper the blue color is



(a)  

7.

Liquid that dissolves the solution

(Hint: always water)

(a)  

8.

Material that gets broken down by the solvent

(a)  

9.

Taking a sample of a solution and diluting it through a series of standard volumes

(Hint: 40 -> 20 -> 10 -> etc)

(a)  

10.

Measures the absorbency of light

(Hint: you can also tell what the percentage of light that was transmitted)

(a)  

11.

Unit 6: Buffering capacity

What is the independent variable?

(a)  

12.

Unit 6: Buffering capacity

What is the dependent variable?

(a)  

13.

  • removes cofactors and prevents them from binding/interacting with protein/enzyme

  • (Hint: was called EGTA)



(a)  

14.

  • metal (non-protein) ions binds to enzymes allows to fill in gaps that missed by substrates

  • Needed for amylase activity



(a)  

15.

  • resistance to pH level change

  • High = strongly resists pH change

    • (Solution E)

  • Low = resistance to pH change

    • (Solution D)



(a)  

16.

  • Due to either different temperature or pH levels

  • When a protein loses its shape And function

  • When there was an increase of leftover starch, shows that the amylase wasn’t working



(a)  

17.

V1=(C2/C1) x V2

(a)  

18.

What is the unit 3 test subject?

(a)  

19.

Unit 3 independent variable

(Hint: daphnia magna)

(a)  

20.

Unit 3: dependent variable

(Hint: daphnia magna)

(a)  

21.

What is the kingdom?

(a)  

22.

What is the domain?

(a)  

23.

What is the species?

(a)  

24.

What is the kingdom?

(a)  

25.

What is the domain?

(a)  

26.

What is the species?

(a)  

27.

What is the kingdom?

(a)  

28.

What is the domain?

(a)  

29.

What is the species?

(a)  

30.

What is the kingdom?

(a)  

31.

What is the domain?

(a)  

32.

What is the species?

(a)  

33.

  • clarity of an image

  • As magnification increases, this also increases



(a)  

34.

  • amount of playroom you have before it unfocuses

  • As magnification increase as it field decreases



(a)  

35.

Ocular lens (10x) times objective lens

(a)  

36.

  • amount of area of the specimen you can see

  • As magnification increase this descrease



(a)  

37.

Is this gram positive or negative?

Due to a thin layer and the cell wall crystal violet is washed away and red appears u

(a)  

38.

  • Stain: crystal violet

  • Thick layer of peptoglycan



(a)